DNA flow cytometry of human spermatozoa: Consistent stoichiometric staining of sperm DNA using a novel decondensation protocol

Cytometry Part A

Volumn 73, Issue 10, 2008, Pages 965-970

  Kovács, Tamás ^a   Békési, Gyöngyi ^a   Fábián, Ákos ^a   Rákosy, Zsuzsa ^a,b   Horváth, Gábor ^a   Mátyus, László ^a   Balázs, Margit ^a,b   Jenei, Attila ^a  

^a UNIVERSITY OF DEBRECEN   (Hungary)

^b UNIVERSITY OF DEBRECEN   (Hungary)

Author keywords

Aneuploid spermatozoa; Diploid spermatozoa; DNA FACS; FISH; Lithium diiodosalicylate; Propidium iodide

Indexed keywords

CELL DNA; PROPIDIUM IODIDE;

ARTICLE; DIPLOIDY; DNA CONTENT; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; FLUORESCENCE IN SITU HYBRIDIZATION; HAPLOIDY; HUMAN; HUMAN CELL; MALE; NORMAL HUMAN; PRIORITY JOURNAL; SPERMATOZOON; STOICHIOMETRY;

DIPLOIDY; DNA; FLOW CYTOMETRY; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; MALE; PROPIDIUM; SALICYLIC ACIDS; SENSITIVITY AND SPECIFICITY; SPERM COUNT; SPERMATOZOA;

EID: 53049099362     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20618     Document Type: Article

References (31)

1. Egozcue J, Blanco J, Vidal F. Chromosome studies in human sperm nuclei using fluorescence in-situ hybridization(FISH). Hum Reprod Update 1997;3:441-452.
2. World Health Organization Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction. Cambridge: University Press; 1999.
3. Ryu HM, Lin WW, Lamb DJ, Chuang W, Lipshultz LI, Bischoff FZ. Increased chromosome X, Y, and 18 nondisjunction in sperm from infertile patients that were identified as normal by strict morphology: Implication for intracytoplasmic sperm injection. Fertil Steril 2001;76:879-883.
4. Jakab A, Kovacs T, Zavaczki Z, Borsos A, Bray-Ward P, Ward D, Huszar G. Efficacy of the swim-up method in eliminating sperm with diminished maturity and aneuploidy. Hum Reprod 2003;18:1481-1488.
5. Egozcue S, Blanco J, Vendrell JM, Garcia F, Veiga A, Aran B, Barri PN, Vidal F, Egozcue J. Human male infertility: Chromosome anomalies, meiotic disorders, abnormal spermatozoa and recurrent abortion. Hum Reprod Update 2000;6:93-105.
6. Hacker-Klom UB, Gohde W, Nieschlag E, Behre HM. DNA flow cytometry of human semen. Hum Reprod 1999;14:2506-2512.
7. Evenson DP, Jost LK, Marshall D, Zinaman MJ, Clegg E, Purvis K, de Angelis P, Claussen OP. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Hum Reprod 1999;14:1039-1049.
8. Evenson DP, Wixon R. Clinical aspects of sperm DNA fragmentation detection and male infertility. Theriogenology 2006;65:979-991.
9. Dean PN, Pinkel D, Mendelsohn ML. Hydrodynamic orientation of sperm heads for flow cytometry. Biophys J 1978;23:7-13.
10. Weissenberg R, Aviram A, Golan R, Lewin LM, Levron J, Madgar I, Dor J, Barkai G, Goldman B. Concurrent use of flow cytometry and fluorescence in-situ hybridization techniques for detecting faulty meiosis in a human sperm sample. Mol Hum Reprod 1998;4:61-66.
11. Levek-Motola N, Soffer Y, Shochat L, Raziel A, Lewin LM, Golan R. Flow cytometry of human semen: A preliminary study of a non-invasive method for the detection of spermatogenetic defects. Hum Reprod 2005;20:3469-3475.
12. Cordelli E, Eleuteri P, Leter G, Rescia M, Spano M. Flow cytometry applications in the evaluation of sperm quality: Semen analysis, sperm function and DNA integrity. Contraception 2005;72:273-279.
13. Kovanci E, Kovacs T, Moretti E, Vigue L, Bray-Ward P, Ward DC, Huszar G. FISH assessment of aneuploidy frequencies in mature and immature human spermatozoa classified by the absence or presence of cytoplasmic retention. Hum Reprod 2001; 16:1209-1217.
14. Juhasz A, Balazs M, Sziklay I, Rakosy Z, Treszl A, Repassy G, Adany R. Chromosomal imbalances in laryngeal and hypopharyngeal cancers detected by comparative genomic hybridization. Cytometry A 2005;67A:151-160.
15. Martin RH, Rademaker A. Reliability of aneuploidy estimates in human sperm: Results of fluorescence in situ hybridization studies using two different scoring criteria. Mol Reprod Dev 1995;42:89-93.
16. Rademaker A, Spriggs E, Ko E, Martin R. Reliability of estimates of diploid human spermatozoa using multicolour fluorescence in-situ hybridization. Hum Reprod 1997;12:77-79.
17. Fossa SD, Melvik JE, Juul NO, Pettersen EO, Theodorsen L. DNA flow cytometry in sperm cells from unilaterally orchiectomized patients with testicular cancer before further treatment. Cytometry 1989;10:345-350.
18. Vicari E, Perdichizzi A, De Palma A, Burrello N, D'Agata R, Calogero AE. Globozoospermia is associated with chromatin structure abnormalities: Case report. Hum Reprod 2002;17:2128-2133.
19. Fuentes-Mascorro G, Serrano H, Rosado A. Sperm chromatin. Arch Androl 2000; 45:215-225.
20. Oliva R, Dixon GH. Vertebrate protamine genes and the histone-to-protamine replacement reaction. Prog Nucleic Acid Res Mol Biol 1991;40:25-94.
21. Aoki VW, Carrell DT. Human protamines and the developing spermatid: Their structure, function, expression and relationship with male infertility. Asian J Androl 2003;5:315-324.
22. Zhao M, Shirley CR, Hayashi S, Marcon L, Mohapatra B, Suganuma R, Behringer RR, Boissonneault G, Yanagimachi R, Meistrich ML. Transition nuclear proteins are required for normal chromatin condensation and functional sperm development. Genesis 2004;38:200-213.
23. Zhang X, San Gabriel M, Zini A. Sperm nuclear histone to protamine ratio in fertile and infertile men: Evidence of heterogeneous subpopulations of spermatozoa in the ejaculate. J Androl 2006;27:414-420.
24. Santisteban MS, Montmasson MP, Giroud F, Ronot X, Brugal G. Fluorescence image cytometry of nuclear DNA content versus chromatin pattern: A comparative study of ten fluorochromes. J Histochem Cytochem 1992;40:1789-1797.
25. Molina J, Castilla JA, Gil T, Hortas ML, Vergara F, Herruzo A. Influence of incubation on the chromatin condensation and nuclear stability of human spermatozoa by flow cytometry. Hum Reprod 1995;10:1280-1286.
26. Eberharter A, Grabher A, Gstraunthaler G, Loidl P. Nuclear matrix of the lower eukaryote Physarum polycephalum and the mammalian epithelial LLC-PK1 cell line. A comprehensive investigation of different preparation procedures. Eur J Biochem 1993;212:573-580.
27. Mirkovitch J, Mirault M-E, Laemmli UK. Organization of the higher-order chromatin loop: Specific DNA attachment sites on nuclear scaffold. Cell 1984;39:223-232.
28. Hossain AM, Rizk B, Behzadian A, Thorneycroft IH. Modified guanidinium thiocyanate method for human sperm DNA isolation. Mol Hum Reprod 1997;3:953-956.
29. Fiorini A, Gouveia Fde S, Fernandez MA. Scaffold/matrix attachment regions and intrinsic DNA curvature. Biochemistry 2006;71:481-488.
30. Smith HC, Ochs RL, Lin D, Chinault AC. Ultrastructural and biochemical comparisons of nuclear matrices prepared by high salt or LIS extraction. Mol Cell Biochem 1987;77:49-61.
31. Perry MJ, Chen X, Lu X. Automated scoring of multiprobe FISH in human spermatozoa. Cytometry A 2007;71A:968-972.