Flow cytometry of human semen: A preliminary study of a non-invasive method for the detection of spermatogenetic defects

Human Reproduction

Volumn 20, Issue 12, 2005, Pages 3469-3475

  Levek Motola, N ^a   Soffer, Y ^b   Shochat, L ^a   Raziel, A ^b   Lewin, L M ^a   Golan, Rachel ^a  

^a TEL AVIV UNIVERSITY   (Israel)

^b ASSAF HAROFEH MEDICAL CENTER   (Israel)

Author keywords

Flow cytometry; Male infertility; Non obstructive azoospermia; Spermatogenesis

Indexed keywords

DOUBLE STRANDED DNA; PROPIDIUM IODIDE; RED FLUORESCENT PROTEIN;

ARTICLE; AZOOSPERMIA; CELL DIFFERENTIATION; CELL MATURATION; CELL SUSPENSION; CHROMATID; CLINICAL ARTICLE; CONTROLLED STUDY; CORRELATION ANALYSIS; DIPLOIDY; FLOW CYTOMETRY; HAPLOIDY; HISTOGRAM; HUMAN; HUMAN CELL; HUMAN TISSUE; INTRACYTOPLASMIC SPERM INJECTION; MALE; MALE INFERTILITY; MEIOSIS; NON INVASIVE MEASUREMENT; OLIGOSPERMIA; PATIENT COUNSELING; PLOIDY; QUANTITATIVE ANALYSIS; RELIABILITY; SEMEN ANALYSIS; SERTOLI CELL; SPERMATID; SPERMATOGENESIS; TESTIS; TETRAPLOIDY;

EID: 28544442596     PISSN: 02681161     EISSN: 14602350     Source Type: Journal    
DOI: 10.1093/humrep/dei247     Document Type: Article

References (28)

1. Brandell RA, Mielnik A, Liotta D, Ye Z, Veeck LL, Palermo GD and Schlegel PN (1998) AZFb deletions predict the absence of spermatozoa with testicular sperm extraction: Preliminary report of a prognostic genetic test. Hum Reprod 13,2812-2815.
2. Coskun S, Tbakhi A, Jaroudi KA, Uzumcu M, Merdad TA and Al-Hussein KA (2002) Flow cytometric ploidy analysis of testicular biopsies from sperm-negative wet preparations. Hum Reprod 17,977-983.
3. Dey P, Mondal AK, Singh SK and Vohra H (2000) Quantitation of sperm-atogenesis by DNA flow cytometry from fine-needle aspiration cytology material. Diagn Cytopathol 23,386-387.
4. Evenson DP, Jost LK, Baer RK, Turner TW and Schrader SM (1991) Individuality of DNA denaturation patterns in human sperm chromatin structure assay. Reprod Toxicol 5,115-125.
5. Fossa SD, Melvik JE, Juul NO, Pettersen EO and Theodorsen L (1989) DNA flow cytometry in sperm cells from unilaterally orchiectomized patients with testicular cancer before further treatment. Cytometry 10,345-350.
6. Friedler S, Raziel A, Strassburger D, Schachter M, Soffer Y and Ron-El R (2002) Factors influencing the outcome of ICSI in patients with obstructive and nonobstructive azoospermia: A comparative study. Hum Reprod 17,114-121.
7. Gillan L, Evans G and Maxwell WMC (2005) Flow cytometric evaluation of sperm parameters in relation to fertility potential. Theriogenology 63,445-457.
8. Gil-Salom M, Romero J, Minguez Y, Molero, MD, Remohi J and Pellicer A (1998) Testicular sperm extraction and intracytoplasmic sperm injection: a chance of fertility in non-obstructive azoospermia. J Urol 160,2063-2067.
9. Giwercman A, Clausen OP, Bruun E, Frimodt-Moller C and Skakkebaek NE (1994) The value of quantitative DNA flow cytometry of testicular fineneedle aspirates in assessment of spermatogenesis: A study of 137 previous maldescended human testes. Int J Androl 17,35-42.
10. Golan R, Weissenberg R, Oschry Y, Shochat L and Lewin LM (2000) Spermatogenesis in the golden hamster during the first spermatogenic wave: A flow cytometric analysis. Mol Reprod Dev 55,205-211.
11. Graham JK (2001) Assessment of sperm quality: A flow cytometry approach. Anim Reprod Sci 68,239-247
12. Hacker-Klom UB, Gohde W, Nieschlag E and Behre HM (1999) DNA flow cytometry of human semen. Hum Reprod 14,2506-2512.
13. Janca FC, Jost LK and Evenson DP (1986) Mouse testicular and sperm cell development characterized from birth to adulthood by dual parameter flow cytometry. Biol Reprod 34,613-623.
14. Kahraman S, Ozgur S, Alatas C, Aksoy S, Balaban B, Evrenkaya T, Nuhoglu A, Tasdemir M, Biberoglu K, Schoysman R, Vanderzwalmen P and Nijs M (1996) High implantation and pregnancy rates with testicular sperm extraction and intracylasmic sperm injection in obstructive and non-obstructive azoospermia. Hum Reprod 11,673-676
15. Katoh C, Kitajima S, SagaY, Kanno J, Hurii I and Inoue T (2002) Assessment of quantitative dual-parameter flow cytometric analysis evaluation of testicular toxicity using cyclophosphamide- and ethinylestradiol-treated rats.J Toxicol Sci 27,87-96.
16. Kostakopoulos A, Protoyerou V, Tekerlekis P, Georgoulakis J, Louras G and Goulandris N (2002) DNA flow cytometric, histological and hormonal analysis of Sertoli cell only syndrome (SECOS). Int Urol Nephrol 33,77-79.
17. Mulhall JP, Burgess CM, Cunningham D, Carson R, Harris D and Oates RD (1997) Presence of mature sperm in testicular parenchyma of men with nonobstructive azoospermia: Prevalence and predictive factors. Urology 49,91-96.
18. Ron-El R, Strassburger D, Friedler S, Komarovski D, Bern O, Soffer Y and Raziel A (1997) Extended sperm preparation: An alternative to testicular sperm extraction in non-obstructive azoospermia. Hum Reprod. 12,1222-1226.
19. Silber SJ and Repping S (2002) Transmission of male infertility to future generations: Lessons from the Y chromosome. Hum Reprod Update 8,217-229.
20. Soffer Y (2004) Nonobstructive azoospermia; predictive factors of testicular sperm retrieval and risks of assisted fertilization [Azoospermies non obstructives; facteurs prédictifs du prélèvement testiculaire et risques de la fécondation assistée] (French, English abstract). Andrologie (SALF) 14, 34-44.
21. Suter L, Bobadilla E, Koch E and Bechter R (1997) Flow cytometric evaluation of the effects of doxorubicin on rat spermatogenesis. Reprod Toxicol 11,521-531.
22. Tournaye H, Verheyen G and Nagy PC (1997) Are there any predictive factors for successful testicular sperm recovery in azoospermic patients? Hum Reprod 12,80-86
23. Vigodner M, Lewin LM, Shochat L, Oschry I, Lotan G, Kleen B and Golan R (2003) Evaluation of damage to the testicular cells of golden hamsters caused by experimental cryptorchidism using flow cytometry and confocal microscopy. Int J Androl 26,84-90.
24. Wald M, Lewin L, Soffer Y, Oschry Y, Shochat L, Wigodner M and Golan R. (2004) Seminal cell characterization using flow cytometry in infertile men. HaRefuah 143,22-25.
25. Weissenberg R, Golan R, Shochat L and Lewin LM (2002) Procarbazine effects on spermatogenesis in golden hamster: A flow cytometric evaluation. Arch Androl 48,91-100.
26. Westlander G, Hamberger L and Hanson C (1999) Diagnostic epididymal and testicular sperm recovery and genetic aspects in azoospermic men. Fertil Steril 14,118-122.
27. World Health Organization (1999) Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction, 4th edn. Cambridge University Press.
28. Yavetz H, Yogev L, Kleiman S, Botchan A, Hauser R, Lessing JB, Paz G and Gamzu R (2001) Morphology of testicular spermatozoa obtained by testicular sperm extraction in obstructive and nonobstructive azoospermic men and its relation to fertilization success in the in vitro fertilization-intra-cytoplasmic sperm injection system. J Androl 22,376-381.