Efficient culture of CD8+ T cells from the islets of NOD mice and their use for the study of autoreactive specificities

Journal of Immunological Methods

Volumn 339, Issue 1, 2008, Pages 66-73

  Jarchum, Irene ^a   Takaki, Toshiyuki ^a   DiLorenzo, Teresa P ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

CD8+ T cell; Islet infiltrate; NOD mice; Type 1 diabetes

Indexed keywords

GAMMA INTERFERON;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CD8+ T LYMPHOCYTE; CELL ACTIVITY; CELL CULTURE; CELL GROWTH; CELL INFILTRATION; CELL ISOLATION; CELL SPECIFICITY; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; INSULIN DEPENDENT DIABETES MELLITUS; NONHUMAN; NONOBESE DIABETIC MOUSE; PANCREAS ISLET CELL; PRIORITY JOURNAL;

ANIMALS; AUTOANTIGENS; AUTOIMMUNITY; CD8-POSITIVE T-LYMPHOCYTES; CELL CULTURE TECHNIQUES; CELLS, CULTURED; DIABETES MELLITUS, EXPERIMENTAL; DIABETES MELLITUS, TYPE 1; HUMANS; INSULIN-SECRETING CELLS; MICE; MICE, INBRED NOD;

MUS;

EID: 53049086810     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2008.08.007     Document Type: Article

References (28)

1. Blancou P., Mallone R., Martinuzzi E., Severe S., Pogu S., Novelli G., Bruno G., Charbonnel B., Dolz M., Chaillous L., van Endert P., and Bach J.M. Immunization of HLA class I transgenic mice identifies autoantigenic epitopes eliciting dominant responses in type 1 diabetes patients. J. Immunol. 178 (2007) 7458
2. + T lymphocytes in spontaneously diabetic HLA-A2 transgenic NOD mice. J. Immunol. 180 (2008) 5430
3. Faveeuw C., Gagnerault M.C., and Lepault F. Isolation of leukocytes infiltrating the islets of Langerhans of diabetes-prone mice for flow cytometric analysis. J. Immunol. Methods 187 (1995) 163
4. Gotoh M., Maki T., Kiyoizumi T., Satomi S., and Monaco A.P. An improved method for isolation of mouse pancreatic islets. Transplantation 40 (1985) 437
5. Han B., Serra P., Amrani A., Yamanouchi J., Maree A.F., Edelstein-Keshet L., and Santamaria P. Prevention of diabetes by manipulation of anti-IGRP autoimmunity: high efficiency of a low-affinity peptide. Nat. Med. 11 (2005) 645
6. Han B., Serra P., Yamanouchi J., Amrani A., Elliott J.F., Dickie P., Dilorenzo T.P., and Santamaria P. Developmental control of CD8 T cell-avidity maturation in autoimmune diabetes. J. Clin. Invest. 115 (2005) 1879
7. Hanninen A., Jalkanen S., Salmi M., Toikkanen S., Nikolakaros G., and Simell O. Macrophages, T cell receptor usage, and endothelial cell activation in the pancreas at the onset of insulin-dependent diabetes mellitus. J. Clin. Invest. 90 (1992) 1901
8. Itoh N., Hanafusa T., Miyazaki A., Miyagawa J., Yamagata K., Yamamoto K., Waguri M., Imagawa A., Tamura S., and Inada M. Mononuclear cell infiltration and its relation to the expression of major histocompatibility complex antigens and adhesion molecules in pancreas biopsy specimens from newly diagnosed insulin-dependent diabetes mellitus patients. J. Clin. Invest. 92 (1993) 2313
9. + T-cells in HLA-A*0201 transgenic NOD mice. Diabetes 56 (2007) 2551
10. Jarchum I., Nichol L., Trucco M., Santamaria P., and DiLorenzo T.P. Identification of novel IGRP epitopes targeted in type 1 diabetes patients. Clin. Immunol. 127 (2008) 359
11. Kikutani H., and Makino S. The murine autoimmune diabetes model: NOD and related strains. Adv. Immunol. 51 (1992) 285
12. Leiter E. The NOD mouse: a model for insulin-dependent diabetes mellitus. In: Coligan J.E., Kruisbeek A.M., Margulies D.H., Shevach E.M., and Strober W. (Eds). Current Protocols in Immunology (1997), John Wiley & Sons, Hoboken 15.9.1
13. high expression as a marker. J. Immunol. 163 (1999) 5708
14. + T cell reactivity to three islet antigens, including the newly identified widely expressed dystrophia myotonica kinase. J. Immunol. 173 (2004) 6727
15. + T-cell responses identify beta-cell autoimmunity in human type 1 diabetes. Diabetes 56 (2007) 613
16. + T-cell responses change after type 1 diabetes diagnosis and treatment. Diabetes 57 (2008) 1312
17. + T cells in NOD mice. Proc. Natl. Acad. Sci. U. S. A. 105 (2008) 6374
18. Pearson T., Markees T.G., Serreze D.V., Pierce M.A., Marron M.P., Wicker L.S., Peterson L.B., Shultz L.D., Mordes J.P., Rossini A.A., and Greiner D.L. Genetic disassociation of autoimmunity and resistance to costimulation blockade-induced transplantation tolerance in nonobese diabetic mice. J. Immunol. 171 (2003) 185
19. Roep B.O. The role of T-cells in the pathogenesis of type 1 diabetes: from cause to cure. Diabetologia 46 (2003) 305
20. Santamaria P., Lewis C., Jessurun J., Sutherland D.E., and Barbosa J.J. Skewed T-cell receptor usage and junctional heterogeneity among isletitis alpha beta and gamma delta T-cells in human IDDM [corrected]. Diabetes 43 (1994) 599
21. Sarukhan A., Bedossa P., Garchon H.J., Bach J.F., and Carnaud C. Molecular analysis of TCR junctional variability in individual infiltrated islets of non-obese diabetic mice: evidence for the constitution of largely autonomous T cell foci within the same pancreas. Int. Immunol. 7 (1995) 139
22. null mice are diabetes and insulitis resistant. Diabetes 43 (1994) 505
23. Signore A., Pozzilli P., Gale E.A., Andreani D., and Beverley P.C. The natural history of lymphocyte subsets infiltrating the pancreas of NOD mice. Diabetologia 32 (1989) 282
24. Somoza N., Vargas F., Roura-Mir C., Vives-Pi M., Fernandez-Figueras M.T., Ariza A., Gomis R., Bragado R., Marti M., and Jaraquemada D. Pancreas in recent onset insulin-dependent diabetes mellitus. Changes in HLA, adhesion molecules and autoantigens, restricted T cell receptor V beta usage, and cytokine profile. J. Immunol. 153 (1994) 1360
25. Takaki T., Marron M.P., Mathews C.E., Guttmann S.T., Bottino R., Trucco M., DiLorenzo T.P., and Serreze D.V. HLA-A*0201-restricted T cells from "humanized" NOD mice recognize autoantigens of potential clinical relevance to type 1 diabetes. J. Immunol. 176 (2006) 3257
26. + T cells, expanded with dendritic cells presenting a single autoantigenic peptide, suppress autoimmune diabetes. J. Exp. Med. 199 (2004) 1467
27. + T cells in the initiation of insulin-dependent diabetes mellitus. Eur. J. Immunol. 26 (1996) 1762
28. + T cells in diabetic nonobese diabetic mice. J. Immunol. 176 (2006) 1637