Quantitative PCR measurements of the effects of introducing inosines into primers provides guidelines for improved degenerate primer design

Journal of Virological Methods

Volumn 153, Issue 2, 2008, Pages 97-103

  Zheng, Linda ^a   Gibbs, Mark J ^a   Rodoni, Brendan C ^b  

^a AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

^b DEPARTMENT OF PRIMARY INDUSTRIES   (Australia)

Author keywords

Comparative quantification; Degenerate primers; Diagnostics; Inosine; Real time PCR; SYBR Green I

Indexed keywords

COMPLEMENTARY DNA; INOSINE DERIVATIVE; VIRUS DNA; VIRUS RNA;

ARTICLE; DNA SYNTHESIS; GENE AMPLIFICATION; MOLECULAR CLONING; NONHUMAN; POTEXVIRUS; PRACTICE GUIDELINE; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN MOTIF; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS GENE; VIRUS ISOLATION;

DNA PRIMERS; DNA, COMPLEMENTARY; INOSINE; ORGANIC CHEMICALS; PLANT DISEASES; PLANT LEAVES; POLYMERASE CHAIN REACTION; POTYVIRUS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, COMPLEMENTARY; RNA, VIRAL; SOLANUM TUBEROSUM; TEMPLATES, GENETIC;

POTATO VIRUS Y;

EID: 53049086506     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2008.07.029     Document Type: Article

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