Electron microscopy and computational studies of Ebh, a giant cell-wall-associated protein from Staphylococcus aureus

Biochemical and Biophysical Research Communications

Volumn 376, Issue 2, 2008, Pages 261-266

  Sakamoto, Sou ^a   Tanaka, Yoshikazu ^a,b   Tanaka, Isao ^b   Takei, Toshiaki ^a   Yu, Jian ^b   Kuroda, Makoto ^c   Yao, Min ^b   Ohta, Toshiko ^d   Tsumoto, Kouhei ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b HOKKAIDO UNIVERSITY   (Japan)

^c NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

^d UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Cell wall associated protein; Ebh; Electron microscopy; Flexibility; Staphylococcus

Indexed keywords

PROTEIN EBH; STAPHYLOCOCCUS PROTEIN A; UNCLASSIFIED DRUG;

AMINO ACID ANALYSIS; ANIMAL CELL; ARTICLE; CRYSTAL STRUCTURE; ELECTRON MICROSCOPY; MATHEMATICAL COMPUTING; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CROSS LINKING; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN STRUCTURE; SIMULATION; STAPHYLOCOCCUS AUREUS; STRUCTURE ANALYSIS;

BACTERIAL PROTEINS; CARRIER PROTEINS; COMPUTATIONAL BIOLOGY; MICROSCOPY, ELECTRON; MODELS, MOLECULAR; PROTEIN CONFORMATION; STAPHYLOCOCCUS AUREUS;

BACTERIA (MICROORGANISMS); STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

EID: 52949129467     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2008.08.117     Document Type: Article

References (13)

1. Dinges M.M., Orwin P.M., and Schlievert P.M. Exotoxins of Staphylococcus aureus. Clin. Microbiol. Rev. 13 (2000) 16-34
2. Hiramatsu K., Aritaka N., Hanaki H., Kawasaki S., Hosoda Y., Hori S., Fukuchi Y., and Kobayashi I. Dissemination in Japanese hospitals of strains of Staphylococcus aureus heterogeneously resistant to vancomycin. Lancet 350 (1997) 1670-1673
3. Kuroda M., Kuroda H., Oshima T., Takeuchi F., Mori H., and Hiramatsu K. Two-component system VraSR positively modulates the regulation of cell-wall biosynthesis pathway in Staphylococcus aureus. Mol. Microbiol. 49 (2003) 807-821
4. Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y., Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., and Hiramatsu K. Whole genome sequencing of methicillin-resistant Staphylococcus aureus. Lancet 357 (2001) 1225-1240
5. Murakami H., Matsumaru H., Kanamori M., Hayashi H., and Ohta T. Cell wall-affecting antibiotics induce expression of a novel gene, drp35, in Staphylococcus aureus. Biochem. Biophys. Res. Commun. 264 (1999) 348-351
6. Manganelli R., and van de Rijn I. Characterization of emb, a gene encoding the major adhesin of Streptococcus defectivus. Infect. Immun. 67 (1999) 50-56
7. Foster T.J., and Hook M. Surface protein adhesins of Staphylococcus aureus. Trends Microbiol. 6 (1998) 484-488
8. Clarke S.R., Harris L.G., Richards R.G., and Foster S.J. Analysis of Ebh, a 1.1-megadalton cell wall-associated fibronectin-binding protein of Staphylococcus aureus. Infect. Immun. 70 (2002) 6680-6687
9. Navarre W.W., and Schneewind O. Proteolytic cleavage and cell wall anchoring at the LPXTG motif of surface proteins in Gram-positive bacteria. Mol. Microbiol. 14 (1994) 115-121
10. Walsh E.J., O'Brien L.M., Liang X., Hook M., and Foster T.J. Clumping factor B, a fibrinogen-binding MSCRAMM (microbial surface components recognizing adhesive matrix molecules) adhesin of Staphylococcus aureus, also binds to the tail region of type I cytokeratin 10. J. Biol. Chem. 279 (2004) 50691-50699
11. Tanaka Y., Sakamoto S., Kuroda M., Goda S., Gao Y.-G., Tsumoto K., Hiragi Y., Yao M., Watanabe N., Ohta T., and Tanaka I. A helical string of alternately connected three-helix bundles for the cell wall-associated adhesion protein Ebh from Staphylococcus aureus. Structure 16 (2008) 488-496
12. Takei T., Okonogi A., Tateno K., Kimura A., Kojima S., Yazaki K., and Miura K. The effects of the side chains of hydrophobic aliphatic amino acid residues in an amphipathic polypeptide on the formation of alpha helix and its association. J. Biochem. 139 (2006) 271-278
13. Kuroda M., Tanaka Y., Aoki R., Shu D., Tsumoto K., and Ohta T. Staphylococcus aureus giant protein Ebh is involved in tolerance to transient hyperosmotic pressure. Biochem. Biophys. Res. Commun. 374 (2008) 237-241