Determination of DNA polymerase and nuclease activities of DNA-dependent polymerases using fluorescence detection under real-time conditions

Biochemistry (Moscow)

Volumn 73, Issue 9, 2008, Pages 1007-1017

  Bragin, A G ^a,b   Glushkov, S A ^a,b   Ivanov, M K ^a,b,c   Krasnov, A A ^b,c   Dymshits, G M ^a,c  

^a INSTITUTE OF CYTOLOGY AND GENETICS   (Russian Federation)

^b Novosibirsk Region   (Russian Federation)

^c NOVOSIBIRSK STATE UNIVERSITY   (Russian Federation)

Author keywords

DNA polymerase activities; Fluorescence detection; Hot start; Nuclease activity

Indexed keywords

FLUORESCENCE; FLUOROPHORES; REACTION RATES;

DNA POLYMERASE; ENDONUCLEASE ACTIVITIES; ESTIMATION OF TEMPERATURES; FLUORESCENCE DETECTION; FLUORESCENCE QUENCHERS; NUCLEASE ACTIVITY; SIMULTANEOUS DETERMINATIONS; SINGLE-STRANDED REGION;

DNA;

DNA DIRECTED DNA POLYMERASE; ENDONUCLEASE; EXONUCLEASE; FLUORESCENT DYE; PRIMER DNA; TAQ POLYMERASE;

ARTICLE; BIOCHEMISTRY; CHEMISTRY; DNA TEMPLATE; FLUORESCENCE; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; TEMPERATURE; TIME;

BIOCHEMISTRY; DNA PRIMERS; DNA-DIRECTED DNA POLYMERASE; ENDONUCLEASES; EXONUCLEASES; FLUORESCENCE; FLUORESCENT DYES; TAQ POLYMERASE; TEMPERATURE; TEMPLATES, GENETIC; TIME FACTORS;

EID: 52649149116     PISSN: 00062979     EISSN: 16083040     Source Type: Journal    
DOI: 10.1134/S0006297908090083     Document Type: Article

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