Cultivation and molecular identification of Ehrlichia canis and Ehrlichia chaffeensis from a naturally co-infected dog in Venezuela

Veterinary Clinical Pathology

Volumn 37, Issue 3, 2008, Pages 258-265

  Gutiérrez, Clara N ^a,b   Martínez, María ^a,b   Sánchez, Erlinda ^a,b   De Vera, Marisol ^c   Rojas, Maria ^d   Ruiz, Joahnny ^a   Triana Alonso, Francisco J ^a,b  

^a BIOMED UC   (Venezuela)

^b Central University of Venezuela ^*  (Venezuela)

^c UNIVERSIDAD CENTRAL DE VENEZUELA   (Venezuela)

^d Fundacion Instituto de Estudios Avanzados IDEA   (Venezuela)

Author keywords

Dog; Ehrlichia; Ehrlichiosis; Morulae; PCR

Indexed keywords

BACTERIAL DNA;

ANIMAL; ANIMAL DISEASE; ARTICLE; CASE REPORT; CLASSIFICATION; DOG; DOG DISEASE; EHRLICHIA CANIS; EHRLICHIA CHAFFEENSIS; EHRLICHIOSIS; FEMALE; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; MONOCYTE; VENEZUELA;

ANIMALS; DNA, BACTERIAL; DOG DISEASES; DOGS; EHRLICHIA CANIS; EHRLICHIA CHAFFEENSIS; EHRLICHIOSIS; FEMALE; MONOCYTES; VENEZUELA;

ANIMALIA; CANIS FAMILIARIS; EHRLICHIA; EHRLICHIA CANIS; EHRLICHIA CHAFFEENSIS; IXODIDA;

EID: 52649144780     PISSN: 02756382     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1939-165X.2008.00046.x     Document Type: Article

References (36)

1. Dumler J, Barbet A, Bekker C, et al. Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila. Int J Syst Evol Microbiol. 2001;51:2145-2165.
2. Kordick SK, Breitschwerdt EB, Hegarty BC, et al. Coinfection with multiple tick-borne pathogens in a walker hound kennel in North Carolina. J Clin Microbiol. 1999;37:2631-2638.
3. Breitschwerdt EB, Hegarty BC, Hancock SI. Sequential evaluation of dogs naturally infected with Ehrlichia canis, Ehrlichia chaffeensis, Ehrlichia equi, Ehrlichia ewingii or Bartonella vinsonii. J Clin Microbiol. 1998;36:2645-2651.
4. Donatien A, Lestoquard A. Existance en Algerie d'une rickettsia du chien [Existence of a rickettsia in dogs of Algeria]. Bull Soc Pathol Exot. 1935;28:418-419.
5. Harrus S, Bark H, Waner T. Canine monocytic ehrlichiosis: an update. Compend Contin Educ Pract Vet. 1997:;19:431-444.
6. Maeda K, Markowitz N, Hawley RC, Ristic M, Cox D, McDade JE. Human infection with Ehrlichia canis, a leukocytic rickettsia. N Engl J Med. 1987;316:853-856.
7. Anderson BE, Dawson JE, Jones DC, Wilson KH. Ehrlichia chaffeensis, a new species associated with human ehrlichiosis. J Clin Microbiol. 1991;29:2838-2842.
8. Lockhart JM, Davidson WR, Stallknecht DE, Dawson JE, Howert EW. Isolation of Ehrlichia chaffeensis from wild white-tailed-deer (Odocoileus virginianus) confirms their role as natural reservoir hosts. J Clin Microbiol. 1997;35:1681-1686.
9. Arraga-Alvarado C. Ehrlichiosis canina en Maracaibo, estado Zulia. Reporte de 55 casos [Canine ehrlichiosis in Maracaibo, Zulia State. Report of 55 cases]. Rev Cient Universidad del Zulia. 1992;2:41-52.
10. Gutierrez N, Martinez M, Arraga-Alvarado C, Bretana A, Pacheco I, Comach G. Ultrastructural identification of Ehrlichia sp in an experimentally infected dog in Venezuela. Invest Clin. 1999;40:165-177.
11. Arraga-AIvarado C, Palmar M, Parra O, Salas P. Ehrlichia platys (Anaplasma platys) in dogs from Maracaibo, Venezuela: an ultrastructural study of experimental and natural infections. Vet Pathol. 2003;40:149-156.
12. Hebert A, Pittman B, Mckinney R, Cherry W. The Preparation and Physico-chemical Characterization of Fluorescent Antibody Reagents. Atlanta, Georgia: U.S. Department of Health, Education, and Welfare. Public Health Service, Center for Disease Control; 1972.
13. Nyindo MB, Ristic M, Huxsoll DL, Smith AR. Tropical canine pancytopenia: in vitro cultivation of the causative agent - Ehrlichia canis. Am J Vet Res. 1971;32:1651-1658.
14. Hemelt IE, Lewis GE, Huxoll DJ, Stephenson EH. Serial propagation of Ehrlichia canis in primary canine peripheral blood monocytes cultures. Cornell Vet. 1980;70:37-42.
15. Dawson JE, Anderson BE, Fishbein DB, et al. Isolation and characterization of an Ehrlichia sp. from a patient diagnosed with human ehrlichiosis. J Clin Microbiol. 1991;29:2741-2745.
16. Tzianabos T, Anderson BE, Mcdade J. Detection of Rickettsia rickettsii in clinical specimens by using polymerase chain reaction technology. J Clin Microbiol. 1989;27:2866-2868.
17. Dawson JE, Stallknecht DE, Howerth EW, et al. Susceptibility of white-tailed deer (Odocoileus virginianus) to infection with Ehrlichia chaffeensis, the etiologic agent of human ehrlichiosis. J Clin Microbiol. 1994;32:2725-2728.
18. Dawson JE, Biggie KL, Warner CK, et al. Polymerase chain reaction evidence of Ehrlichia chaffeensis, an etiologic agent of human ehrlichiosis, in dogs from southeast Virginia. Am J Vet Res. 1996;57:1175-1179.
19. Wen B, Rikihisa Y, Mott JM, et al. Comparison of nested PCR with Immunofluorescent-antibody assay for detection of Ehrlichia canis infection in dogs treated with doxycycline. J Clin Microbiol. 1997;35:1852-1855.
20. Anderson BE, Summer JW, Dawson JE, et al. Detection of the etiologic agent of human ehrlichiosis by polymerase chain reaction. J Clin Microbiol. 1992;30:775-780.
21. Harrus S, Waner T, Aizenberg I, Foley JE, Poland AM, Bark H. Amplification of ehrlichial DNA from dogs 34 months after infection with Ehrlichia canis. J Clin Microbiol. 1998;36:73-76.
22. Suksawat J, Pitulle C, Arraga-Alvarado C, Madrigal K, Hancock S, Breitschwerdt E. Coinfection with three Ehrlichia species in dogs from Thailand and Venezuela with emphasis on consideration of 16S ribosomal DNA secondary structure. J Clin Microbiol. 2001;39:90-93.
23. Ndip LM, Ndip RN, Esemu SN, et al. Ehrlichial infection in Cameroonian canines by Ehrlichia canis and Ehrlichia ewingii. Vet Microbiol. 2005;1-2:59-66.
24. Arraga-Alvarado C, Montero-Ojeda M, Bernardoni A, Anderson BE, Parra O. Human ehrlichiosis: report of the 1st case in Venezuela. Invest Clin. 1996;37:35-49.
25. Ripoll C, Remondegui C, Ordonez G, et al. Evidence of Rickettsial spotted fever and ehrlichial infections a subtropical territory of Jujuy, Argentina. Am J Trop Med Hyg. 1999;61:350-354.
26. Gongora-Biachi RA, Zavala-Velazquez J, Castro-Sansores CJ, Gonzalez-Martinez P. First case of human ehrlichiosis in Mexico. Emerg Infect Dis. 1999;5:481.
27. Calic SB, Galvao MA, Bacellar F, et al. Human ehrlichioses in Brazil: first suspect cases. Braz J Infect Dis. 2004;3:259-62.
28. da Costa PS, Valle LM, Brigatte ME, Greco DB. More about human monocytotropic ehrlichiosis in Brazil: serological evidence of nine new cases. Braz J Infect Dis. 2006;1:7-10.
29. Galvao M, Lamounier J, Tropia M, et al. Rickettsioses emergentes e reemergentes numa região endêmica do Estado de Minas Gerais, Brasil [Emerging and reemerging rickettsiosis in an endemic area of Minas Gerais State, Brazil]. Cad Saude Publica. 2002;18:1593-1597.
30. Lopez J, Rivera M, Concha JC, Gatica S, Loeffeholz M, Barriga O. Serologic evidence for human Ehrlichiosis in Chile. Rev Med Chil. 2003;131:67-70.
31. Unver A, Ferez M, Orellana N, Hung H, Rikihisa Y. Molecular and antigenic comparison of Ehrlichia canis isolates from dogs, ticks, and human in Venezuela. J Clin Microbiol. 2001;39:2788-2793.
32. Ewing SA, Dawson JE, Kocan AA, et al. Experimental transmission of Ehrlichia chaffeensis (Rickettsiales: Ehrlichieae) among white-tailed deer by Amblyomma americanum (Acari: Ixodidae). J Med Entomol. 1995;3:368-374.
33. Kramer VL, Randolph MP, Hui LT, Irwin WE, Gutierrez AG, Vugia DJ. Detection of the agents of human ehrlichiosis in ixodid ticks from California. Am J Trop Med Hyg. 1999;60:62-65.
34. Cao WC, Gao YM, Zhang PH, et al. Identification of Ehrlichia chaffeensis by nested PCR in ticks from southern China. J Clin Microbiol. 2000;38:2778-2780.
35. Ndip LM, Ndip RN, Ndive VE, Awuh JA, Walker DH, McBride JW. Ehrlichia species in Rhipicephalus sanguineus ticks in Cameroon. Vector Borne Zoonotic Dis. 2007;2:221-227.
36. Guerrero R. Las garrapatas de Venezuela (Acarina: Ixodoidea). Listado de especies y claves para su clasificación [Ticks in Venezuela (Acarina: Ixodoidea). List of species and keys for identification]. Bol Dir Malarial San Amb. 1996;36:1-24.