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This was thought to have resulted from a change in surface modification, i.e, a possible loss in the coupled heterostructure between NP and enzyme during ultracentrifugation. After being ultracentrifuged at 15 000 rpm, the Pt NPs still exhibited the average nanocrystal size of ca. 4.0 nm Figures 1 and 2, but they were no longer readily solvated in aqueous medium and resuspension required sonication; possibly indicating some NP agglomeration. By comparison, prior to this procedure, the Pt NP-GOx as-prepared structures were fully solvated forming a golden-brown solution as shown in Supporting Information Figure SI. This apparent change in solvation properties led us to believe that there may have been a change in surface modification by this procedure which prevented NP assembly on PDDA, explaining the lack of measurable electrocatalytic activity of the film thus assembled
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This was thought to have resulted from a change in surface modification, i.e., a possible loss in the coupled heterostructure between NP and enzyme during ultracentrifugation. After being ultracentrifuged at 15 000 rpm, the Pt NPs still exhibited the average nanocrystal size of ca. 4.0 nm (Figures 1 and 2), but they were no longer readily solvated in aqueous medium and resuspension required sonication; possibly indicating some NP agglomeration. By comparison, prior to this procedure, the Pt NP-GOx as-prepared structures were fully solvated forming a golden-brown solution as shown in Supporting Information Figure SI. This apparent change in solvation properties led us to believe that there may have been a change in surface modification by this procedure which prevented NP assembly on PDDA, explaining the lack of measurable electrocatalytic activity of the film thus assembled.
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