Yak lactate dehydgogenase A4: Purification, properties, and cDNA cloning

Bioscience, Biotechnology and Biochemistry

Volumn 72, Issue 9, 2008, Pages 2448-2451

  Zheng, Yucai ^a   Si, Xiaohui ^a   He, Qinghua ^a   Jin, Suyu ^a   Hong, Jian ^a  

^a SOUTHWEST UNIVERSITY FOR NATIONALITIES   (China)

Author keywords

Lactate dehydrogenase A; Molecular adaptation; Purification; Yak

Indexed keywords

AMINES; AMINO ACIDS; CLONING; ORGANIC ACIDS; PURIFICATION;

AMINO ACID SUBSTITUTIONS; CDNA CLONING; LACTATE DEHYDROGENASE; LACTATE DEHYDROGENASE A; MICHAELIS CONSTANTS; MOLECULAR ADAPTATION; PYRUVATE; SKELETAL MUSCLES; YAK;

GENETIC ENGINEERING;

COMPLEMENTARY DNA; ISOENZYME; LACTATE DEHYDROGENASE; LACTIC ACID; PYRUVIC ACID;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMAL; ARTICLE; CATTLE; CHEMICAL STRUCTURE; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; SEQUENCE HOMOLOGY; SKELETAL MUSCLE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; CATTLE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ISOENZYMES; KINETICS; L-LACTATE DEHYDROGENASE; LACTIC ACID; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUSCLE, SKELETAL; PYRUVIC ACID; SEQUENCE HOMOLOGY, AMINO ACID;

BOS; BOS GRUNNIENS;

EID: 52649128817     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.80208     Document Type: Article

References (16)

1. Golding, G. B., and Dean, A. M., The structural basis of molecular adaptation. Mol. Biol. Evol., 15, 355-369 (1998).
2. Nowak, R. M., Artiodactyla; Bovidae; Genus Bos. "Walker's Mammals of the World," The Johns Hopkins University Press, Baltimore and London, pp. 1424-1431 (1991).
3. Tu, Z. C., Qiu, H., and Zhang, Y. P., Polymorphism in mitochondrial DNA (mtDNA) of yak (Bos grunniens). Biochem. Genet., 40, 187-193 (2002).
4. Li, S. S., Fitch, W. M., Pan, Y. C., and Sharief, F. S., Evolutionary relationships of vertebrate lactate dehydrogenase isozymes A4 (muscle), B4 (heart), and C4 (testis). J. Biol. Chem., 258, 7029-7032 (1983).
5. Crawford, D. L., Constantino, H. R., and Powers, D. A., Lactate dehydrogenase-B cDNA from the teleost Fundulus heteroclitus: evolutionary implications. Mol. Biol. Evol., 6, 369-383 (1989).
6. Ishiguro, N., Osame, S., Kagiya, R., Ichijo, S., and Shinagawa, M., Primary structure of bovine lactate dehydrogenase-A isozyme and its synthesis in Escherichia coli. Gene, 91, 281-285 (1990).
7. Tsoi, S. C., and Li, S. S., The nucleotide and deduced amino-acid sequences of a cDNA encoding lactate dehydrogenase from Caenorhabditis elegans: the evolutionary relationships of lactate dehydrogenases from mammals, birds, amphibians, fish, nematodes, plants, bacteria, mycoplasma, and plasmodium. Biochem. Biophys. Res. Commun., 205, 558-564 (1994).
8. Wigley, D. B., Clarke, A. R., Dunn, C. R., Barstow, D. A., Atkinson, T., Chia, W. N., Muirhead, H., and Holbrook, J. J., The engineering of a more thermally stable lactate dehydrogenase by reduction of the area of a water-accessible hydrophobic surface. Biochim. Biophys. Acta, 916, 145-148 (1987).
9. Wigley, D. B., Gamblin, S. J., Turkenburg, J. P., Dodson, E. J., Piontek, K., Muirhead, H., and Holbrook, J. J., Structure of a ternary complex of an allosteric lactate dehydrogenase from Bacillus stearothermophilus at 2.5 A resolution. J. Mol. Biol., 223, 317-335 (1992).
10. Feeney, R., Clarke, A. R., and Holbrook, J. J., A single amino acid substitution in lactate dehydrogenase improves the catalytic efficiency with an alternative coenzyme. Biochem. Biophys. Res. Commun., 166, 667-672 (1990).
11. Deng, H., Zheng, J., Clarke, A., Holbrook, J. J., Callender, R., and Burgner, J. W., Source of catalysis in the lactate dehydrogenase system: ground-state interactions in the enzyme-substrate complex. Biochemistry, 33, 2297-2305 (1994).
12. Nicholls, D. J., Davey, M., Jones, S. E., Miller, J., Holbrook, J. J., Clarke, A. R., Scawen, M. D., Atkinson, T., and Goward, C. R., Substitution of the amino acid at position 102 with polar and aromatic residues influences substrate specificity of lactate dehydrogenase. J. Protein Chem., 13, 129-133 (1994).
13. Dissing, U., and Mattiasson, B., Integrated removal of nucleic acids and recovery of LDH from homogenate of beef heart by affinity precipitation. Bioseparation, 7, 221-229 (1998).
14. Burgos, C., Maldonado, C., Gerez de Burgos, N. M., Aoki, A., and Blanco, A., Intracellular localization of the testicular and sperm-specific lactate dehydrogenase isozyme C4 in mice. Biol. Reprod., 53, 84-92 (1995).
15. Marchat, L., Loiseau, P. M., and Petek, F., Purification and characterization of lactate dehydrogenase isoenzymes 1 and 2 from Molinema dessetae (Nematoda: Filarioidea). Parasitol. Res., 82, 672-680 (1996).
16. Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254 (1976).