Chapter Eighteen Methods to Dissect Mitochondrial Membrane Permeabilization in the Course of Apoptosis

Methods in Enzymology

Volumn 442, Issue , 2008, Pages 355-374

  Galluzzi, Lorenzo ^a   Vitale, Ilio ^a   Kepp, Oliver ^a   Séror, Claire ^a   Hangen, Emilie ^a   Perfettini, Jean Luc ^a   Modjtahedi, Nazanine ^a   Kroemer, Guido ^a  

^a Institut Gustave Roussy   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; CALCEIN; CASPASE; MEMBRANE PROTEIN; CARRIER PROTEIN; MITOCHONDRIAL PERMEABILITY TRANSITION PORE;

APOPTOSIS; CELL CYCLE; CELL DEATH; CELL MEMBRANE PERMEABILITY; CELL METABOLISM; CELL STRUCTURE; CYTOFLUOROMETRY; ENZYME ACTIVATION; ENZYME SYNTHESIS; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE MICROSCOPY; MITOCHONDRIAL MEMBRANE; MITOCHONDRIAL MEMBRANE POTENTIAL; PRIORITY JOURNAL; PROTEIN SECRETION; REVIEW; ANIMAL; ARTICLE; FLUORESCENT ANTIBODY TECHNIQUE; METABOLISM; MITOCHONDRION; PERMEABILITY; PHYSIOLOGY; TRANSPORT AT THE CELLULAR LEVEL;

ANIMALS; APOPTOSIS; BIOLOGICAL TRANSPORT; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; MEMBRANE POTENTIAL, MITOCHONDRIAL; MITOCHONDRIA; MITOCHONDRIAL MEMBRANE TRANSPORT PROTEINS; MITOCHONDRIAL MEMBRANES; PERMEABILITY;

EID: 52249104691     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(08)01418-3     Document Type: Review

References (18)

1. Castedo M., Ferri K., Roumier T., Metivier D., Zamzami N., and Kroemer G. Quantitation of mitochondrial alterations associated with apoptosis. J. Immunol. Methods 265 (2002) 39-47
2. Cossarizza A., Baccarani-Contri M., Kalashnikova G., and Franceschi C. A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzim idazolcarbocyanine iodide (JC-1). Biochem. Biophys. Res. Commun. 197 (1993) 40-45
3. Galluzzi L., Zamzami N., de La Motte Rouge T., Lemaire C., Brenner C., and Kroemer G. Methods for the assessment of mitochondrial membrane permeabilization in apoptosis. Apoptosis 12 (2007) 803-813
4. Garrido C., Galluzzi L., Brunet M., Puig P.E., Didelot C., and Kroemer G. Mechanisms of cytochrome c release from mitochondria. Cell Death Differ. 13 (2006) 1423-1433
5. Green D., and Kroemer G. The central executioners of apoptosis: Caspases or mitochondria. Trends Cell Biol. 8 (1998) 267-271
6. Kroemer G., Galluzzi L., and Brenner C. Mitochondrial membrane permeabilization in cell death. Physiol. Rev. 87 (2007) 99-163
7. Li L.Y., Luo X., and Wang X. Endonuclease G is an apoptotic DNase when released from mitochondria. Nature 412 (2001) 95-99
8. Macho A., Decaudin D., Castedo M., Hirsch T., Susin S.A., Zamzami N., and Kroemer G. Chloromethyl-X-rosamine is an aldehyde-fixable potential-sensitive fluorochrome for the detection of early apoptosis. Cytometry 25 (1996) 333-340
9. Martins L.M., Iaccarino I., Tenev T., Gschmeissner S., Totty N.F., Lemoine N.R., Savopoulos J., Gray C.W., Creasy C.L., Dingwall C., and Downward J. The serine protease Omi/HtrA2 regulates apoptosis by binding XIAP through a reaper-like motif. J. Biol. Chem. 277 (2002) 439-444
10. Metivier D., Dallaporta B., Zamzami N., Larochette N., Susin S.A., Marzo I., and Kroemer G. Cytofluorometric detection of mitochondrial alterations in early CD95/Fas/APO-1-triggered apoptosis of Jurkat T lymphoma cells: Comparison of seven mitochondrion-specific fluorochromes. Immunol. Lett. 61 (1998) 157-163
11. Modjtahedi N., Giordanetto F., Madeo F., and Kroemer G. Apoptosis-inducing factor: Vital and lethal. Trends Cell Biol. 16 (2006) 264-272
12. Petronilli V., Miotto G., Canton M., Brini M., Colonna R., Bernardi P., and Di Lisa F. Transient and long-lasting openings of the mitochondrial permeability transition pore can be monitored directly in intact cells by changes in mitochondrial calcein fluorescence. Biophys. J. 76 (1999) 725-734
13. Poncet D., Boya P., Metivier D., Zamzami N., and Kroemer G. Cytofluorometric quantitation of apoptosis-driven inner mitochondrial membrane permeabilization. Apoptosis 8 (2003) 521-530
14. Ravagnan L., Roumier T., and Kroemer G. Mitochondria, the killer organelles and their weapons. J. Cell. Physiol. 192 (2002) 131-137
15. Susin S.A., Zamzami N., and Kroemer G. Mitochondria as regulators of apoptosis: Doubt no more. Biochim. Biophys. Acta 1366 (1998) 151-165
16. Verhagen A.M., Ekert P.G., Pakusch M., Silke J., Connolly L.M., Reid G.E., Moritz R.L., Simpson R.J., and Vaux D.L. Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins. Cell 102 (2000) 43-53
17. Zamzami N., Marchetti P., Castedo M., Decaudin D., Macho A., Hirsch T., Susin S.A., Petit P.X., Mignotte B., and Kroemer G. Sequential reduction of mitochondrial transmembrane potential and generation of reactive oxygen species in early programmed cell death. J. Exp. Med. 182 (1995) 367-377
18. Zamzami N., Marchetti P., Castedo M., Zanin C., Vayssiere J.L., Petit P.X., and Kroemer G. Reduction in mitochondrial potential constitutes an early irreversible step of programmed lymphocyte death in vivo. J. Exp. Med. 181 (1995) 1661-1672