Generation and evaluation of movement protein-specific single-chain antibodies for delaying symptoms of Tomato spotted wilt virus infection in tobacco

Plant Pathology

Volumn 57, Issue 5, 2008, Pages 854-860

  Zhang, M Y ^a,c   Zimmermann, S ^a,d   Fischer, R ^a,b   Schillberg, S ^b  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b FRAUNHOFER INSTITUTE FOR MOLECULAR BIOLOGY AND APPLIED ECOLOGY IME   (Germany)

^c NATIONAL CANCER INSTITUTE   (United States)

^d MILTENYI BIOTEC GMBH   (Germany)

Author keywords

Disease resistance; Phage display; Recombinant antibodies; Tospovirus; Transgenic plants

Indexed keywords

ANTIBODY; BACTERIUM; DISEASE RESISTANCE; PROTEIN; RECOMBINATION; TOBACCO; TRANSGENIC PLANT; VEGETABLE; VIRAL DISEASE; VIRUS;

BACTERIA (MICROORGANISMS); NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; TOMATO SPOTTED WILT VIRUS; TOSPOVIRUS;

EID: 52149116031     PISSN: 00320862     EISSN: 13653059     Source Type: Journal    
DOI: 10.1111/j.1365-3059.2008.01863.x     Document Type: Article

References (34)

1. Bajrovic K, Erdag B, Atalay EO, Cirakoclu B, 2001. Full resistance to tobacco mosaic virus infection conferred by the transgenic expression of a recombinant antibody in tobacco. Biotechnology and Biotechnological Equipment 15, 21 7.
2. Beachy RN, 1999. Coat-protein-mediated resistance to tobacco mosaic virus: discovery mechanisms and exploitation. Philosophical Transactions of the Royal Society B, Biological Sciences 354, 659 64.
3. Boonrod K, Galetzka D, Nagy PD, Conrad U, Krczal G, 2004. Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance. Nature Biotechnology 22, 856 62.
4. De Jaeger G, Buys E, Eeckhout D et al., 1999. High level accumulation of single-chain variable fragments in the cytosol of transgenic Petunia hybrida. European Journal of Biochemistry 259, 426 34.
5. Fecker LF, Kaufmann A, Commandeur U, Commandeur J, Koenig R, Burgermeister W, 1996. Expression of single-chain antibody fragments (scFv) specific for beet necrotic yellow vein virus coat protein or 25 kDa protein in Escherichia coli and Nicotiana benthamiana. Plant Molecular Biology 32, 979 86.
6. Fecker LF, Koenig R, Obermeier C, 1997. Nicotiana benthamiana plants expressing beet necrotic yellow vein virus (BNYVV) coat protein-specific scFv are partially protected against the establishment of the virus in the early stages of infection and its pathogenic effects in the late stages of infection. Archives of Virology 142, 1857 63.
7. Fischer R, Drossard J, Liao Y, Schillberg S, 1998. Characterization and application of plant-derived recombinant antibodies. In: Cunningham C, Porter AJR, eds. Methods in Biotechnology, Vol. 3: Recombinant Proteins from Plants: Production and Isolation of Clinically Useful Compounds. Totowa, NJ, USA: Humana Press, 129 42.
8. Goldbach R, Bucher E, Prins M, 2003. Resistance mechanisms to plant viruses: an overview. Virus Research 92, 207 12.
9. Hochuli E, Bannwarth W, Döbeli H, Gentz R, Stüber D, 1988. Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent. Biotechnology 6, 1321 5.
10. Höfgen R, Willmitzer L, 1988. Storage of competent cells for Agrobacterium transformation. Nucleic Acids Research 16, 9877.
11. Horsch RB, Fry JE, Hoffmann NL, Rogers SG, Fraley RT, 1985. A simple and general method for transferring genes into plants. Science 227, 1229 31.
12. Jan FJ, Fagoaga C, Pang SZ, Gonsalves D, 2000. A minimum length of N gene sequence in transgenic plants is required for RNA-mediated tospovirus resistance. Journal of General Virology 81, 235 42.
13. Kay R, Chan A, Daly M, McPherson J, 1987. Duplication of CaMV 35S promoter sequences creates a strong enhancer for plant genes. Science 236, 1299 302.
14. M protein of tomato spotted wilt virus (TSWV), a putative viral movement protein. Virology 200, 56 65.
15. Krubphachaya P, Juricek M, Kertbundit S, 2007. Induction of RNA-mediated resistance to papaya ringspot virus type W. Journal of Biochemistry and Molecular Biology 40, 404 11.
16. Nölke G, Fischer R, Schillberg S, 2004. Antibody-based pathogen resistance in plants. Journal of Plant Pathology 86, 5 17.
17. Nölke G, Schneider B, Fischer R, Schillberg S, 2005. Immunomodulation of polyamine biosynthesis in tobacco plants has a significant impact on polyamine levels and generates a dwarf phenotype. Plant Biotechnology Journal 3, 237 47.
18. Owen M, Gandecha A, Cockburn B, Whitelam G, 1992. Synthesis of a functional anti-phytochrome single-chain Fv protein in transgenic tobacco. Biotechnology (NY) 10, 790 4.
19. Prins M, Lohuis D, Schots A, Goldbach R, 2005. Phage display-selected single-chain antibodies confer high levels of resistance against Tomato spotted wilt virus. Journal of General Virology 86, 2107 13.
20. Schillberg S, Zimmermann S, Findlay K, Fischer R, 2000. Plasma membrane display of anti-viral single chain Fv fragments confers resistance to tobacco mosaic virus. Molecular Breeding 6, 317 26.
21. Schillberg S, Zimmermann S, Voss A, Fischer R, 1999. Apoplastic and cytosolic expression of full-size antibodies and antibody fragments in Nicotiana tabacum. Transgenic Research 8, 255 63.
22. Schillberg S, Zimmermann S, Zhang MY, Fischer R, 2001. Antibody-based resistance to plant pathogens. Transgenic Research 10, 1 12.
23. Schmitz J, Prufer D, Rohde W, Tacke E, 1996. Non-canonical translation mechanisms in plants: efficient in vitro and in planta initiation at AUU codons of the tobacco mosaic virus enhancer sequence. Nucleic Acids Research 24, 257 63.
24. Schouten A, Roosien J, Bakker J, Schots A, 2002. Formation of disulfide bridges by a single-chain Fv antibody in the reducing ectopic environment of the plant cytosol. Journal of Biological Chemistry 277, 19339 45.
25. Soellick T, Uhrig JF, Bucher GL, Kellmann JW, Schreier PH, 2000. The movement protein NSm of tomato spotted wilt tospovirus (TSWV): RNA binding, interaction with the TSWV N protein, and identification of interacting plant proteins. Proceedings of the National Academy of Sciences, USA 97, 2373 8.
26. Tavladoraki P, Benvenuto E, Trinca S, De Martinis D, Cattaneo A, Galeffi P, 1993. Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack. Nature 366, 469 72.
27. Tavladoraki P, Girotti A, Donini M et al., 1999. A single-chain antibody fragment is functionally expressed in the cytoplasm of both Escherichia coli and transgenic plants. European Journal of Biochemistry 262, 617 24.
28. Villani ME, Roggero P, Bitti O, Benvenuto E, Franconi R et al., 2005. Iomodulation of cucumber mosaic virus infection by intrabodies selected in vitro from a stable single-framework phage display library. Plant Molecular Biology 58, 305 16.
29. Voss A, Niersbach M, Hain R et al., 1995. Reduced virus infectivity in Nicotiana tabacum secreting a TMV-specific full-size antibody. Molecular Breeding 1, 39 50.
30. Xiao XW, Chu PWG, Frenkel MJ, Tabe LM, Shukla DD, Hanna PJ, Higgins TJV, Müller WJ, Ward CW, 2000. Antibody-mediated improved resistance to CIYVV and PVY infections in transgenic tobacco plants expressing a single-chain variable region antibody. Molecular Breeding 6, 421 31.
31. Zhang M, Schillberg S, Prins M, Fischer R, 1999. Optimizing expression of a rare codon-rich viral protein in Escherichia coli using the IMPACT system. Analytical Biochemistry 271, 202 4.
32. Zhang M, Schillberg S, Zimmermann S, Liao Y, Breuer G, Fischer R, 2001. GST fusion proteins cause false positives during selection of viral movement protein specific single chain antibodies. Journal of Virological Methods 91, 139 47.
33. Zimmermann S, Emans N, Fischer R, Schillberg S, 2006. Plantibody-based disease resistance in plants. In: Tuzun S, Bent E, eds. Multigenic and Induced Systemic Resistance in Plants. Berlin, Germany: Springer, 456 76.
34. Zimmermann S, Schillberg S, Liao YC, Fischer R, 1998. Intracellular expression of TMV-specific single-chain Fv fragments leads to improved virus resistance in Nicotiana tabacum. Molecular Breeding 4, 369 79.