GST fusion proteins cause false positives during selection of viral movement protein specific single chain antibodies

Journal of Virological Methods

Volumn 91, Issue 2, 2001, Pages 139-147

  Zhang, Mei Yun ^a   Schillberg, Stefan ^a,b   Zimmermann, Sabine ^a   Liao, Yu Cai ^a   Breuer, Gudrun ^a   Fischer, Rainer ^a,b  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b IUCT   (Germany)

Author keywords

GST fusion protein; Phage ELISA; scFv; Viral movement protein

Indexed keywords

GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; IMMUNOGLOBULIN FRAGMENT; SINGLE CHAIN ANTIBODY; UNCLASSIFIED DRUG; VIRAL MOVEMENT PROTEIN; VIRUS PROTEIN;

ACCURACY; ANALYTICAL ERROR; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIBODY DETECTION; ARTICLE; CHICKEN; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; NONHUMAN; PHAGE DISPLAY; PLANT VIRUS; PRIORITY JOURNAL; SPLEEN;

ANIMALS; ANTIBODIES, VIRAL; BACTERIOPHAGES; BINDING SITES; CHICKENS; CLONING, MOLECULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; FALSE POSITIVE REACTIONS; GLUTATHIONE TRANSFERASE; IMMUNOGLOBULIN FRAGMENTS; PEPTIDE LIBRARY; PLANT VIRAL MOVEMENT PROTEINS; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; SOLUBILITY; TOSPOVIRUS; VIRAL PROTEINS;

LYCOPERSICON ESCULENTUM; TOMATO SPOTTED WILT VIRUS;

EID: 0035146679     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(00)00262-7     Document Type: Article

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