A new gibberellin detection system in living cells based on antibody VH/VL interaction

Biochemical and Biophysical Research Communications

Volumn 376, Issue 1, 2008, Pages 134-138

  Lee, Younggiu ^a   Asami, Tadao ^a   Yamaguchi, Isomaro ^a   Ueda, Hiroshi ^b   Suzuki, Yoshihito ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

Gibberellin; Open sandwich ELISA; Protein complementation assay

Indexed keywords

GIBBERELLIN; GIBBERELLIN A4; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; IMMUNOGLOBULIN ANTIBODY; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIOPHAGE; CARBOXY TERMINAL SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FLUORESCENCE; IMMUNOGLOBULIN VARIABLE REGION; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FRAGMENT COMPLEMENTATION ASSAY; PROTEIN INDUCTION;

ANTIBODIES; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GIBBERELLINS; GREEN FLUORESCENT PROTEINS; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN LIGHT CHAINS; IMMUNOGLOBULIN VARIABLE REGION; PEPTIDE LIBRARY; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI;

EID: 52049113642     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2008.08.130     Document Type: Article

References (15)

1. Hasegawa M., Hashimoto M., Zhang J., Nakajima M., Takeda K., Yamaguchi I., and Murofushi N. Immunohistochemistry of gibberellins in rice anthers. Biosci. Biotechnol. Biochem. 59 (1995) 1925-1929
2. Micknick S.W. Exploring protein interactions by interaction-induced folding of proteins from complementary peptide fragments. Curr. Opin. Struct. Biol. 11 (2001) 472-477
3. Pollok B.A., and Heim R. Using GFP in FRET-based applications. Trends Cell Biol. 9 (1999) 57-60
4. Lalonde S., Ehrhardt D.W., Loqué D., Chen J., Rhee S.Y., and Frommer W.B. Molecular and cellular approaches for the detection of protein-protein interactions: latest techniques and current limitations. Plant J. 53 (2008) 610-635
5. Ueda H., Tsumoto K., Kubota K., Suzuki E., Nagamune T., Nishimura H., Schueler P.A., Winter G., Kumagai I., and Mahoney W.C. Open sandwich ELISA: a novel immunoassay based on the interachain interaction of antibody variable region. Nat. Biotechnol. 14 (1996) 1714-1718
6. Ueda H. Open sandwich immunoassay: a novel immunoassay approach based on the interchain interaction of an antibody variable region. J. Biosci. Bioeng. 94 2 (2002) 614-619
7. Suzuki Y., Ito S., Otsuka K., Iwasawa E., Nakajima M., and Yamaguchi I. Preparation of functional single-chain antibodies against bioactive gibberellins by utilizing randomly mutagenized phage-display libraries. Biosci. Biotechnol. Biochem. 69 (2005) 610-619
8. Aburatani T., Sakamoto K., Masuda K., Nishi K., Ohkawa H., Nagamune T., and Ueda H. A general method to select antibody fragments suitable for noncompetitive detection of monovalent antigens. Anal. Chem. 75 (2003) 4057s-4064s
9. Nissim A., Hoogenboom H.R., Tomlinson I.M., Flynn G., Midfley C., Lane D., and Winter G. Antibody fragments from a 'single pot' phage display library as immunochemical reagents. EMBO J. 13 (1994) 692-698
10. Chiu W., Niwa Y., Zeng W., Hirano T., Kobayashi H., and Sheen J. Engineered GFP as a vital reporter in plants. Curr. Biol. 6 (1996) 325-330
11. 4 and their use in immunoaffinity column chromatography. Plant Cell Physiol. 32 (1991) 515-521
12. Suzuki Y., Mizuno T., Urakami E., Yamaguchi I., and Asami T. Immunomodulation of bioactive gibberellin confers gibberellin-deficient phenotypes in plants. Plant Biotechnol. J. 6 (2008) 355-367
13. Ghosh I., Hamilton A.D., and Regan L. Antiparallel leucine zipper-directed protein reassembly: application to the green fluorescent protein. J. Am. Chem. Soc. 122 (2000) 5658-5659
14. Magliery T.J., Wilson C.G., Pan W.M., Mishler D., Ghosh I., Hamilton A.D., and Regan L. Detecting protein-protein interactions with a green fluorescent protein fragment reassembly trap: scope and mechanism. J. Am. Chem. Soc. 127 (2005) 146-157
15. 4 antibody 4-B8(8)/E9 Fab fragment. Biochem. Biophys. Res. Commun. 293 (2002) 489-496