In-Gel Detection of S-Nitrosated Proteins Using Fluorescence Methods

Methods in Enzymology

Volumn 441, Issue , 2008, Pages 53-71

  Kettenhofen, Nicholas J ^a   Wang, Xunde ^b   Gladwin, Mark T ^b,c   Hogg, Neil ^a  

^a FREE RADICAL RESEARCH CENTER   (United States)

^b NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

^c NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASCORBIC ACID; BIOTIN; COPPER ION; CYANINE DYE; METAL ION; S NITROSOTHIOL; THIOL; TRACE METAL;

COMPARATIVE STUDY; FLUORESCENCE; GEL ELECTROPHORESIS; HUMAN; NITROSATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN MODIFICATION; REDUCTION; REVIEW;

ANIMALS; GELS; HUMANS; NITROSATION; PROTEINS; S-NITROSOTHIOLS; SPECTROMETRY, FLUORESCENCE;

EID: 52049105346     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(08)01204-4     Document Type: Chapter

References (19)

1. Alban A., David S.O., Bjorkesten L., Andersson C., Sloge E., Lewis S., and Currie I. A novel experimental design for comparative two-dimensional gel analysis: Two-dimensional difference gel electrophoresis incorporating a pooled internal standard. Proteomics 3 (2003) 36-44
2. 2+ of nitric oxide release from S-nitrosothiols (RSNO). J. Chem. Soc. Perkin Trans. 2 (1995) 741-745
3. Bryan N.S., and Grisham M.B. Methods to detect nitric oxide and its metabolites in biological samples. Free Radic. Biol. Med. 43 (2007) 645-657
4. Fang K., Ragsdale N.V., Carey R.M., Macdonald T., and Gaston B. Reductive assays for S-nitrosothiols: Implications for measurements in biological systems. Biochem. Biophys. Res. Commun. 252 (1998) 535-540
5. Forrester M.T., Foster M.W., and Stamler J.S. Assessment and application of the biotin switch technique for examining protein S-nitrosylation under conditions of pharmacologically induced oxidative stress. J. Biol. Chem. 282 (2007) 13977-13983
6. Gladwin M.T., Wang X., and Hogg N. Methodological vexation about thiol oxidation versus S-nitrosation: A commentary on "An ascorbate-dependent artifact that interferes with the interpretation of the biotin-switch assay.". Free Radic Biol. Med. 41 (2006) 557-561
7. Greco T.M., Hodara R., Parastatidis I., Heijnen H.F.G., Dennehy M.K., Liebler D.C., and Ischiropoulos H. Identification of S-nitrosylation motifs by site-specific mapping of the S-nitrosocysteine proteome in human vascular smooth muscle cells. Proc. Natl. Acad. Sci. USA. 103 (2006) 7420-7425
8. Hao G., Derakhshan B., Shi L., Campagne F., and Gross S.S. SNOSID, a proteomic method for identification of cysteine S-nitrosylation sites in complex protein mixtures. Proc. Natl. Acad. Sci. USA 103 (2006) 1012-1017
9. Holmes A.J., and Williams D.L.H. Reaction of ascorbic acid with S-nitrosothiols: Clear evidence for two distinct reaction pathways. J. Chem. Soc. Perkin Trans. 2 (2000) 1639-1644
10. Jaffrey S.R., Erdjument-Bromage H., Ferris C.D., Tempst P., and Snyder S.H. Protein S-nitrosylation: A physiological signal for neuronal nitric oxide. Nat. Cell Biol. 3 (2001) 193-197
11. Kashiba-Iwatsuki M., Yamaguchi M., and Inoue M. Role of ascorbic acid in the metabolism of S-nitroso-glutathione. FEBS Lett. 389 (1996) 149-152
12. Kettenhofen N.J., Broniowska K.A., Keszler A., Zhang Y., and Hogg N. Proteomic methods for analysis of S-nitrosation. J. Chromatogr. B 851 (2007) 152-159
13. Landar A., Oh J.Y., Giles N.M., Isom A., Kirk M., Barnes S., and Darley-Usmar V.M. A sensitive method for the quantitative measurement of protein thiol modification in response to oxidative stress. Free Radic. Biol. Med. 40 (2006) 459-468
14. Maeda K., Finnie C., and Svensson B. Cy5 maleimide labelling for sensitive detection of free thiols in native protein extracts: Identification of seed proteins targeted by barley thioredoxin h isoforms. Biochem. J. 378 (2004) 497-507
15. Samouilov A., and Zweier J.L. Development of chemiluminescence-based methods for specific quantitation of nitrosylated thiols. Anal. Biochem. 258 (1998) 322-330
16. Singh R.J., Hogg N., Joseph J., and Kalyanaraman B. Mechanism of nitric oxide release from S-nitrosothiols. J. Biol. Chem. 271 (1996) 18596-18603
17. Wang X., Kettenhofen N.J., Shiva S., Hogg N., and Gladwin M.T. Free Radic Biol. Med 44 7 (2003) 1362-1372
18. Yang B.K., Vivas E.X., Reiter C.D., and Gladwin M.T. Methodologies for the sensitive and specific measurement of S-nitrosothiols, iron-nitrosyls, and nitrite in biological samples. Free Radic. Res. 37 (2003) 1-10
19. Zhang Y., Keszler A., Broniowska K.A., and Hogg N. Characterization and application of the biotin-switch assay for the identification of S-nitrosated proteins. Free Radic. Biol. Med. 38 (2005) 874-881