Cy5 maleimide labelling for sensitive detection of free thiols in native protein extracts: Identification of seed proteins targeted by barley thioredoxin h isoforms

Biochemical Journal

Volumn 378, Issue 2, 2004, Pages 497-507

  Maeda, Kenji ^a   Finnie, Christine ^a   Svensson, Birte ^a  

^a CARLSBERG LABORATORY   (Denmark)

Author keywords

Barley seed proteome; Cy5 maleimide labelling; Disulphide reduction; Monobromobimane; Target proteins; Thioredoxin h

Indexed keywords

DYES; ENZYME KINETICS; MASS SPECTROMETRY; SEED;

GERMINATION; ISOENZYMES;

PROTEINS;

AMYLASE INHIBITOR; CHITINASE; CYCLOPHILIN; EMBRYO SPECIFIC PROTEIN; FLUORESCENT DYE; GLYOXALASE; GLYOXALASE LIKE PROTEIN; ISOENZYME; ISOPROTEIN; LIPID TRANSFER PROTEIN; MALEIMIDE; PROTEIN; PROTEOME; SUBTILISIN; SUPEROXIDE DISMUTASE; THIOL DERIVATIVE; THIOREDOXIN; TRYPSIN INHIBITOR; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

ARTICLE; BARLEY; DATA BASE; GEL; GERMINATION; ISOTOPE LABELING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MATURATION; NUCLEOTIDE SEQUENCE; PLANT SEED; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN TARGETING; TWO DIMENSIONAL ELECTROPHORESIS;

BICYCLO COMPOUNDS; CARBOCYANINES; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FLUORESCENT DYES; HORDEUM; MALEIMIDES; PLANT EXTRACTS; PLANT PROTEINS; PROTEIN ISOFORMS; PROTEOME; SEEDS; SULFHYDRYL COMPOUNDS; THIOREDOXIN;

HORDEUM VULGARE SUBSP. VULGARE;

EID: 1642409412     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20031634     Document Type: Article

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