Evolution reversed: The ability to bind iron restored to the N-lobe of the murine inhibitor of carbonic anhydrase by strategic mutagenesis

Biochemistry

Volumn 47, Issue 37, 2008, Pages 9847-9855

  Mason, Anne B ^a   Judson, Gregory L ^b   Bravo, Maria Cristina ^a   Edelstein, Andrew ^b   Byrne, Shaina L ^a   James, Nicholas G ^a   Roush, Eric D ^c,f   Fierke, Carol A ^d   Bobst, Cedric E ^e   Kaltashov, Igor A ^e   Daughtery, Margaret A ^b  

^a UNIVERSITY OF VERMONT   (United States)

^b MIDDLEBURY COLLEGE   (United States)

^c DUKE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d UNIVERSITY OF MICHIGAN   (United States)

^e UNIVERSITY OF MASSACHUSETTS   (United States)

^f Biacore Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ABSORPTION; AMINES; AMINO ACIDS; ELECTROSPRAY IONIZATION; GENE ENCODING; HEMODYNAMICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INDEPENDENT COMPONENT ANALYSIS; IONS; LIGANDS; MASS SPECTROMETRY; METALS; MICA; NEGATIVE IONS; ORGANIC ACIDS;

ABSORPTION AND FLUORESCENCE SPECTRA; CARBONIC ANHYDRASE; CONFORMATIONAL CHANGES; ELECTROSPRAY IONIZATION MASS SPECTROMETRY; FAMILY MEMBERS; FERRIC IONS; FERRIC IRON; INHIBITORY ACTIVITIES; IRON TRANSPORT; IRON-BINDING; ISOFORMS; METAL BINDING; SYNERGISTIC ANIONS; TRANSFERRIN (TF);

SILICATE MINERALS;

ARGININE; CARBONATE DEHYDRATASE; CARBONATE DEHYDRATASE INHIBITOR; CARBONIC ACID; FERRIC ION; TRANSFERRIN; TRYPSIN;

ABSORPTION SPECTROSCOPY; ARTICLE; BINDING AFFINITY; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; ENDOSOME; ENZYME INHIBITION; HUMAN; IRON BINDING CAPACITY; IRON KINETICS; IRON STORAGE; METAL BINDING; MOLECULAR EVOLUTION; MUTAGENESIS; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN STRUCTURE; SPECTROFLUOROMETRY;

ANIMALS; BINDING SITES; CARBONIC ANHYDRASE II; CARBONIC ANHYDRASES; EVOLUTION; HUMANS; HYDROGEN-ION CONCENTRATION; IRON; LIGANDS; MICE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; RATS; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

CANIS FAMILIARIS; MURINAE; MUS; RATTUS; SUIDAE; SUS;

EID: 51849155074     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi801133d     Document Type: Article

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