A generally applicable sequential alkaline phosphatase immunohistochemical double staining

Journal of Histotechnology

Volumn 31, Issue 3, 2008, Pages 119-127

  Van Der Loos, Chris M ^a   Teeling, Peter ^a  

^a ACADEMIC MEDICAL CENTER   (Netherlands)

Author keywords

Alkaline phosphatase; Animal tissue; Co localization; Double staining; Heat induced epitope retrieval; Human tissue

Indexed keywords

ALKALINE PHOSPHATASE; ANTIBODY; EPITOPE; FORMALDEHYDE; PARAFFIN;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENZYME MECHANISM; FROZEN SECTION; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MOUSE; NEUTRON RADIATION; NONHUMAN;

ANIMALIA;

EID: 51849089193     PISSN: 01478885     EISSN: None     Source Type: Journal    
DOI: 10.1179/his.2008.31.3.119     Document Type: Article

References (18)

1. Nakane PK: Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method: a study on pituitary glands in the rat. J Histochem Cytochem 16:557-559, 1968
2. Tramu G, Pillez A, Leonardelli J: An efficient method of antibody elution for the successive or simultaneous localization of two antigens by immunocytochemistry. J Histochem Cytochem 26:322-324, 1978
3. Van der Loos CM: Immunoenzyme Multiple Staining Techniques. Royal Microscopy Handbook no. 45. Oxford, UK: BIOS Scientific Publishers, 1999
4. Sternberger LA, Joseph SA: The unlabeled antibody method. Contrasting color staining of paired pituitary hormones without antibody removal. J Histochem Cytochem 27:1424-1429, 1979
5. Van der Loos CM: Multiple immunoenzyme staining: methods for the observation with spectral imaging. J Histochem Cytochem 56:313-328, 2008
6. Van der Loos CM: Multiple staining in molecular morphology. In: Molecular Morphology in Human Tissues: Techniques and Applications. (G.W. Hacker and R.R. Tubbs, eds). Boca Raton, FL: CRC Press, 2005, pp 27-63
7. Boorsma DM: Direct immunoenzyme double staining applicable for monoclonal antibodies. Histochemistry 80:103-106, 1984
8. Van der Loos CM, Das PK, Houthoff H-J: An immunoenzyme triple staining method using both polyclonal and monoclonal antibodies from the same species. Application of combined direct, indirect and avidin-biotin complex (ABC) technique. J Histochem Cytochem 35:1199-1204, 1987
9. Feller AC, Parwaresch MR, Wacker H-H, Radzun J-J, Lennert K: Combined immunohistochemical staining for surface IgD and T-lymphocyte subsets with monoclonal antibodies in human tonsils. Histochem J 15:557-562, 1983
10. Lan HY, Mu W, Nikolic-Paterson DJ, Atkins RC: A novel, simple, reliable, and sensitive method for multiple immunoenzyme staining: use of microwave oven heating to block antibody crossreactivity and retrieve antigens. J Histochem Cytochem 43:97-102, 1995
11. Van den Brink GR, Tytgat KMAJ, Van der Hulst RWM, Van der Loos CM, Einerhand AWC, Büller HA, et al.: H. pylori colocalises with MUC5AC in the human stomach. Gut 46:601-607, 2000
12. Levenson RM, Mansfield JR: Multispectral imaging in biology and medicine: slices of life. Cytometry A 69:748-758, 2006
13. Yamashita S, Okada Y: Application of heat-induced antigen retrieval to aldehyde-fixed fresh frozen sections. J Histochem Cytochem 53:1421-1432, 2005
14. Gray DS, Selbie D, Cooper RF, Williams M, Robson G, Doyle E: Simultaneous de-waxing and standardization of antigen retrieval in immunohistochemistry using commercially available equipment. Immunocytochemistry 4:93-97, 2006
15. Shi S-R, Cote RJ, Taylor CR: Antigen retrieval immunohistochemistry: past, present and future. J Histochem Cytochem 45:327-343, 1987
16. Rouschop KMA, Claessen N, Pals ST, Weening JJ, Florquin S: CD44-disruption prevents degeneration of the capillary network in obstructive nephropathy via regulation of TGF-b1 induced apoptosis. J Am Soc Nephrol 17:746-753, 2006
17. Schnell SA, Staines WA, Wessendorf MW: Reduction of lipofuchin-like autofluorescence in fluorescently labeled tissue. J Histochem Cytochem 47:719-730, 1999
18. Bauer M, Schilling N, Spanel-Borowski K: Limitation of microwave treatment for double immunolabelling with antibodies of the same species and isotype. Histochem Cell Biol 116:227-232, 2001