A transient assay system for the assessment of cell-autonomous gene function in dehydration-stressed barley

Journal of Experimental Botany

Volumn 59, Issue 12, 2008, Pages 3359-3369

  Marzin, Stephan ^a   Mihaly, Robert ^a,b   Pauk, Janos ^b   Schweizer, Patrick ^a  

^a LEIBNIZ INSTITUTE OF PLANT GENETICS AND CROP PLANT RESEARCH IPK   (Germany)

^b CEREAL RESEARCH NON PROFIT COMPANY   (Hungary)

Author keywords

DsRed; Particle bombardment; RNAi; Single cell

Indexed keywords

FLUORESCENT PROTEIN 583; GREEN FLUORESCENT PROTEIN; PHOTOPROTEIN; VEGETABLE PROTEIN; WATER;

ARTICLE; BARLEY; DISASTER; EVALUATION; FLUORESCENCE MICROSCOPY; GENE EXPRESSION REGULATION; GENETICS; LUMINESCENCE; METABOLISM; METHODOLOGY; PHYSIOLOGY; PLANT LEAF; REPORTER GENE;

GENE EXPRESSION REGULATION, PLANT; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HORDEUM; LUMINESCENT MEASUREMENTS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; NATURAL DISASTERS; PLANT LEAVES; PLANT PROTEINS; WATER;

HORDEUM; TRITICUM AESTIVUM;

EID: 51749084690     PISSN: 00220957     EISSN: 14602431     Source Type: Journal    
DOI: 10.1093/jxb/ern186     Document Type: Article

References (52)

1. Abebe T, Guenzi AC, Martin B, Cushman JC. 2003. Tolerance of mannitol-accumulating transgenic wheat to water stress and salinity. Plant Physiology 131, 1748-1755.
2. Araus JL, Slafer GA, Reynolds MP, Royo C. 2002. Plant breeding and drought in C-3 cereals: what should we breed for? Annals of Botany 89, 925-940.
3. Bahieldin A, Mahfouz HT, Eissa HF, Saleh OM, Ramadan AM, Ahmed IA, Dyer WE, El-Itriby HA, Madkour MA. 2005. Field evaluation of transgenic wheat plants stably expressing the HVA1 gene for drought tolerance. Physiologia Plantarum 123, 421-427.
4. Baird GS, Zacharias DA, Tsien RY. 2000. Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral. Proceedings of the National Academy of Sciences, USA 97, 11984-11989.
5. Barrs HD, Weatherley PE. 1962. A re-examination of relative turgidity technique for estimating water deficits in leaves. Australian Journal of Biological Sciences 15, 413.
6. Brigneti G, Martin-Hernandez AM, Jin HL, Chen J, Baulcombe DC, Baker B, Jones JDG. 2004. Virus-induced gene silencing in Solanum species. The Plant Journal 39, 264-272.
7. Brini F, Hanin M, Mezghani I, Berkowitz GA, Masmoudi K. 2007. Overexpression of wheat Na+/H+ antiporter TNHX1 and H+-pyrophosphatase TVP1 improve salt- and drought-stress tolerance in Arabidopsis thaliana plants. Journal of Experimental Botany 58, 301-308.
8. Broin M, Rey P. 2003. Potato plants lacking the CDSP32 plastidic thioredoxin exhibit overoxidation of the BAS1 2-cysteine peroxiredoxin and increased lipid peroxidation in thylakoids under photooxidative stress. Plant Physiology 132, 1335-1343.
9. Caldwell DG, McCallum N, Shaw P, Muehlbauer GJ, Marshall DF, Waugh R. 2004. A structured mutant population for forward and reverse genetics in barley (Hordeum vulgare L.). The Plant Journal 40, 143-150.
10. Cattivelli L, Baldi P, Crosatti C, Di Fonzo N, Faccioli P, Grossi M, Mastrangelo AM, Pecchioni N, Stanca AM. 2002. Chromosome regions and stress-related sequences involved in resistance to abiotic stress in Triticeae. Plant Molecular Biology 48, 649-665.
11. Choi DW, Zhu B, Close TJ. 1999. The barley (Hordeum vulgare L.) dehydrin multigene family: sequences, allele types, chromosome assignments, and expression characteristics of 11 Dhn genes of cv Dicktoo. Theoretical and Applied Genetics 98, 1234-1247.
12. Devi SR, Chen X, Oliver DJ, Xiang CB. 2006. A novel high-throughput genetic screen for stress-responsive mutants of Arabidopsis thaliana reveals new loci involving stress responses. The Plant Journal 47, 652-663.
13. Diab AA, Teulat-Merah B, This D, Ozturk NZ, Benscher D, Sorrells ME. 2004. Identification of drought-inducible genes and differentially expressed sequence tags in barley. Theoretical and Applied Genetics 109, 1417-1425.
14. Dong WB, Nowara D, Schweizer P. 2006. Protein polyubiquitination plays a role in basal host resistance of barley. The Plant Cell 18, 3321-3331.
15. Douchkov D, Nowara D, Zierold U, Schweizer P. 2005. A high-throughput gene-silencing system for the functional assessment of defense-related genes in barley epidermal cells. Molecular Plant-Microbe Interactions 18, 755-761.
16. Fu DL, Huang BR, Xiao YM, Muthukrishnan S, Liang GH. 2007. Overexpression of barley hva1 gene in creeping bentgrass for improving drought tolerance. Plant Cell Reports 26, 467-477.
17. Fukuda A, Chiba K, Maeda M, Nakamura A, Maeshima M, Tanaka Y. 2004. Effect of salt and osmotic stresses on the expression of genes for the vacuolar H+-pyrophosphatase, H+-ATPase subunit A, and Na+/H+ antiporter from barley. Journal of Experimental Botany 55, 585-594.
18. Gao SQ, Xu HJ, Cheng XG, Chen M, Xu ZS, Li LC, Ye XG, Du LP, Hao XY, Ma YZ. 2005. Improvement of wheat drought and salt tolerance by expression of a stress-inducible transcription factor GmDREB of soybean (Glycine max). Chinese Science Bulletin 50, 2714-2723.
19. Gross LA, Baird GS, Hoffman RC, Baldridge KK, Tsien RY. 2000. The structure of the chromophore within DsRed, a red fluorescent protein from coral. Proceedings of the National Academy of Sciences, USA 97, 11990-11995.
20. Ihlow A, Schweizer P, Seiffert U. 2008. A high-throughput screening system for barley/powdery mildew interactions based on automated analysis of light micrographs. BMC Plant Biology 8.
21. Ingram J, Bartels D. 1996. The molecular basis of dehydration tolerance in plants. Annual Review of Plant Physiology and Plant Molecular Biology 47, 377-403.
22. Karim S, Aronsson H, Ericson H, Pirhonen M, Leyman B, Welin B, Mantyla E, Palva ET, Van Dijck P, Holmstrom KO. 2007. Improved drought tolerance without undesired side effects in transgenic plants producing trehalose. Plant Molecular Biology 64, 371-386.
23. Oh SJ, Kwon CW, Choi DW, Song SI, Kim JK. 2007. Expression of barley HvCBF4 enhances tolerance to abiotic stress in transgenic rice. Plant Biotechnology Journal 5, 646-656.
24. Panstruga R. 2004. A golden shot: how ballistic single cell transformation boosts the molecular analysis of cereal-mildew interactions. Molecular Plant Pathology 5, 141-148.
25. Panstruga R, Kim MC, Cho MJ, Schulze-Lefert P. 2003. Testing the efficiency of dsRNAi constructs in vivo: a transient expression assay based on two fluorescent proteins. Molecular Biology Reports 30, 135-140.
26. Park S, Li JS, Pittman JK, Berkowitz GA, Yang HB, Undurraga S, Morris J, Hirschi KD, Gaxiola RA. 2005. Upregulation of a H+-pyrophosphatase (H+-PPase) as a strategy to engineer drought-resistant crop plants. Proceedings of the National Academy of Sciences, USA 102, 18830-18835.
27. Pellegrineschi A, Reynolds M, Pacheco M, Brito RM, Almeraya R, Yamaguchi-Shinozaki K, Hoisington D. 2004. Stress-induced expression in wheat of the Arabidopsis thaliana DREB1A gene delays water stress symptoms under greenhouse conditions. Genome 47, 493-500.
28. Peng YH, Lin WL, Cai WM, Arora R. 2007. Overexpression of a Panax ginseng tonoplast aquaporin alters salt tolerance, drought tolerance and cold acclimation ability in transgenic Arabidopsis plants. Planta 226, 729-740.
29. Perruc E, Charpenteau M, Ramirez BC, Jauneau A, Galaud JP, Ranjeva R, Ranty B. 2004. A novel calmodulin-binding protein functions as a negative regulator of osmotic stress tolerance in Arabidopsis thaliana seedlings. The Plant Journal 38, 410-420.
30. Qian G, Han ZX, Zhao T, Deng GB, Pan ZF, Yu MQ. 2007. Genotypic variability in sequence and expression of HVA1 gene in Tibetan hulless barley, Hordeum vulgare ssp vulgare, associated with resistance to water deficit. Australian Journal of Agricultural Research 58, 425-431.
31. Radchuk R, Radchuk V, Weschke W, Borisjuk L, Weber H. 2006. Repressing the expression of the SUCROSE NONFERMENTING-1-RELATED PROTEIN KINASE gene in pea embryo causes pleiotropic defects of maturation similar to an abscisic acid-insensitive phenotype. Plant Physiology 140, 263-278.
32. Reyes JL, Rodrigo MJ, Colmenero-Flores JM, Gil JV, Garay-Arroyo A, Campos F, Salamini F, Bartels D, Covarrubias AA. 2005. Hydrophilins from distant organisms can protect enzymatic activities from water limitation effects in vitro. Plant, Cell and Environment 28, 709-718.
33. Riera M, Valon C, Fenzi F, Giraudat J, Leung J. 2005. The genetics of adaptive responses to drought stress: abscisic acid-dependent and abscisic acid-independent signalling components. Physiologia Plantarum 123, 111-119.
34. Sakuma Y, Maruyama K, Osakabe Y, Qin F, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. 2006a. Functional analysis of an Arabidopsis transcription factor, DREB2A, involved in drought-responsive gene expression. The Plant Cell 18, 1292-1309.
35. Sakuma Y, Maruyama K, Qin F, Osakabe Y, Shinozaki K, Yamaguchi-Shinozaki K. 2006b. Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress- responsive gene expression. Proceedings of the National Academy of Sciences, USA 103, 18822-18827.
36. Schweizer P, Pokorny J, Abderhalden O, Dudler R. 1999. A transient assay system for the functional assessment of defense-related genes in wheat. Molecular Plant-Microbe Interactions 12, 647-654.
37. Shen QH, Saijo Y, Mauch S, Biskup C, Bieri S, Keller B, Seki H, Ulker B, Somssich IE, Schulze-Lefert P. 2007. Nuclear activity of MLA immune receptors links isolate-specific and basal disease-resistance responses. Science 315, 1098-1103.
38. Shinozaki K, Yamaguchi-Shinozaki K. 2007. Gene networks involved in drought stress response and tolerance. Journal of Experimental Botany 58, 221-227.
39. Silva JM, Mizuno H, Brady A, Lucito R, Hannon GJ. 2004. RNA interference microarrays: high-throughput loss-of-function genetics in mammalian cells. Proceedings of the National Academy of Sciences, USA 101, 6548-6552.
40. Skinner JS, von Zitzewitz J, Szucs P, Marquez-Cedillo L, Filichkin T, Amundsen K, Stockinger EJ, Thomashow MF, Chen THH, Hayes PM. 2005. Structural, functional, and phylogenetic characterization of a large CBF gene family in barley. Plant Molecular Biology 59, 533-551.
41. Suprunova T, Krugman T, Fahima T, Chen G, Shams I, Korol A, Nevo E. 2004. Differential expression of dehydrin genes in wild barley, Hordeum spontaneum, associated with resistance to water deficit. Plant, Cell and Environment 27, 1297-1308.
42. Talame V, Ozturk NZ, Bohnert HJ, Tuberosa R. 2007. Barley transcript profiles under dehydration shock and drought stress treatments: a comparative analysis. Journal of Experimental Botany 58, 229-240.
43. Teulat B, Zoumarou-Wallis N, Rotter B, Ben Salem M, Bahri H, This D. 2003. QTL for relative water content in field-grown barley and their stability across Mediterranean environments. Theoretical and Applied Genetics 108, 181-188.
44. Tondelli A, Francia E, Barabaschi D, Aprile A, Skinner JS, Stockinger EJ, Stanca AM, Pecchioni N. 2006. Mapping regulatory genes as candidates for cold and drought stress tolerance in barley. Theoretical and Applied Genetics 112, 445-454.
45. Trujillo M, Altschmied L, Schweizer P, Kogel KH, Huckelhoven R. 2006. Respiratory burst oxidase homologue A of barley contributes to penetration by the powdery mildew fungus Blumeria graminis f. sp hordei. Journal of Experimental Botany 57, 3781-3791.
46. Warringer J, Ericson E, Fernandez L, Nerman O, Blomberg A. 2003. High-resolution yeast phenomics resolves different physiological features in the saline response. Proceedings of the National Academy of Sciences, USA 100, 15724-15729.
47. Xu DP, Duan XL, Wang BY, Hong BM, Ho THD, Wu R. 1996. Expression of a late embryogenesis abundant protein gene, HVA1, from barley confers tolerance to water deficit and salt stress in transgenic rice. Plant Physiology 110, 249-257.
48. Xu P, Zhang Y, Kang L, Roossinck MJ, Mysore KS. 2006. Computational estimation and experimental verification of off-target silencing during posttranscriptional gene silencing in plants. Plant Physiology 142, 429-440.
49. Xue GP, Loveridge CW. 2004. HvDRF1 is involved in abscisic acid-mediated gene regulation in barley and produces two forms of AP2 transcriptional activators, interacting preferably with a CT-rich element. The Plant Journal 37, 326-339.
50. Yamauchi D, Zentella R, Ho TH. 2002. Molecular analysis of the barley (Hordeum vulgare L.) gene encoding the protein kinase PKABA1 capable of suppressing gibberellin action in aleurone layers. Planta 215, 319-326.
51. Zhao T, Palotta M, Langridge P, Prasad M, Graner A, Schulze-Lefert P, Koprek T. 2006. Mapped Ds/T-DNA launch pads for functional genomics in barley. The Plant Journal 47, 811-826.
52. Zimmermann G, Baumlein H, Mock HP, Himmelbach A, Schweizer P. 2006. The multigene family encoding germin-like proteins of barley. Regulation and function in basal host resistance. Plant Physiology 142, 181-192.