A highly sensitive single-enzyme homocysteine assay

Nature Protocols

Volumn 3, Issue 9, 2008, Pages 1388-1394

  Tan, Yuying ^a   Hoffman, Robert M ^a,b  

^a ANTICANCER INC   (United States)

^b UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME; HOMOCYSTEINE; HOMOCYSTEINE ALPHA,GAMMA LYASE; HYDROGEN SULFIDE; UNCLASSIFIED DRUG;

ACCURACY; AMINO ACID BLOOD LEVEL; ANALYZER; ARTICLE; CHROMATOPHORE; CONTROLLED STUDY; ENZYMATIC ASSAY; FLUORESCENT READER; HIGH THROUGHPUT SCREENING; HOMOCYSTEINE BLOOD LEVEL; HUMAN; INTERMETHOD COMPARISON; PRIORITY JOURNAL; SENSITIVITY ANALYSIS;

BLOOD CHEMICAL ANALYSIS; FLUORESCENCE; HOMOCYSTEINE; HYDROGEN SULFIDE; LYASES; MOLECULAR STRUCTURE;

EID: 51649102863     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2008.117     Document Type: Article

References (35)

1. McCully, K.S. Homocysteine and vascular disease. Nat. Med. 2 386-389 (1996).
2. Nurk, E. et al. Plasma total homocysteine and hospitalizations for cardiovascular disease: The Hordaland Homocysteine Study. Arch. Intern. Med. 162, 1374-1381 (2002).
3. Selhub, J. et al. Association between plasma homocysteine concentrations and extracranial carotid-artery stenosis. N. Engl. J. Med. 332, 286-291 (1995).
4. Nygard, O. et al. Plasma homocysteine levels and mortality in patients with coronary artery disease. N. Engl. J. Med. 337, 230-236 (1997).
5. Malinow, M.R. et al. Reduction of plasma homocysteine levels by breakfast cereal fortified with folic acid in patients with coronary heart disease. N. Engl. J. Med. 338, 1009-1015 (1998).
6. Lindner, A., Bankson, D.D., Stehman-Breen, C., Mahuren, J.D. & Coburn, S.P. Vitamin B6 metabolism and homocysteine in end-stage renal disease and chronic renat insufficiency. Am. J. Kidney Dis. 39, 134-145 (2002).
7. Refsum, H., Wesenberg, F. & Ueland, P.M. Plasma homocysteine in children with acute lymphoblastic leukemia: Changes during a chemotherapeutic regimen including methotrexate. Cancer Res. 51, 828-835 (1991).
8. Geisler, J. et al. Changes in folate status as determined by reduction in total plasma homocysteine levels during leucovorin modulation of 5-fluorouracil therapy in cancer patients. Clin. Cancer Res. 4, 2125-2128 (1998).
9. Sun, C.F., Haven, T.R., Wu, T.L., Tsao, K.C. & Wu, J.T. Serum total homocysteine increases with the rapid proliferation rate of tumor cells and decline upon cell death: A potential new tumor marker. Clin. Chim. Acta. 321, 55-62 (2002).
10. Clarke, R. et al. Folate vitamin B12, and serum total homocysteine levels in confirmed Alzheimer disease. Arch. Neurol. 55, 1449-1455 (1998).
11. Vafai, S.B. & Stock, J.B. Protein phosphatase 2A methylation: A link between elevated plasma homocysteine and Alzheimer's disease. FEBS Lett. 518, 1-4 (2002).
12. Selhub, J. Homocysteine metabolism. Annu. Rev. Nutr. 19, 217-246 (1999).
13. Duan, W. et al. Dietary folate deficiency and elevated homocysteine levels endanger dopaminergic neurons in models of Parkinson's disease. J. Neurochem. 80, 101-110 (2002).
14. Allain, P. et al. Sulfate and cysteine levels in the plasma of patients with Parkinson's disease. Neurotoxicology 16, 527-529 (1995).
15. Wollesen, F. et al. Plasma total homocysteine and cysteine in relation to glomerular filtration ratein diabetes mellitus. Kidney Int. 55, 1028-1035 (1999).
16. Stehouwer, C.D., Gall, M.A., Hougaard, P., Jakobs, C. & Parving, H.H. Plasma homocysteine concentration predicts mortality in non-insulin-dependent diabetic patients with and without albuminuria. Kidney Int. 55, 308-314 (1999).
17. Vollset, S.E. et al. Plasma total homocysteine, pregnancy complications, and adverse pregnancy outcomes: The Hordaland homocysteine study. Am. J. Clin. Nutr. 71, 962-968 (2000).
18. Righetti, M., Serbelloni, P., Milani, S. & Ferrario, G. Homocysteine-lowering vitamin B treatment decreases cardiovascular events in hemodialysis patients. Blood Purif. 24, 379-386 (2006).
19. Ubbink, J.B., Hayward Vermaak, W.J. & Bissbort, S. Rapid high-performance liquid chromatographic assay for total homocysteine levels in human serum. J. Chromatogr. 565, 441-446 (1991).
20. Ueland, P.M. et al. Total homocysteine in plasma or serum: Methods and clinical applications. Clin. Chem. 39, 1764-1779 (1993).
21. Fiskerstrand, T., Refsum, H., Kvalheim, G. & Ueland, P.M. Homocysteine and other thiols in plasma and urine: Automated determination and sample stability. Clin. Chem. 39, 263-271 (1993).
22. Dudman, N.P.B., Guo, X.W., Crooks, R., Xie, L. & Silberberg, J.S. Assay of plasma homocysteine: Light sensitivity of the fluorescent 7-benzo-2-oxa-1, 3-diazole-4-sulfonic acid derivative, and use of appropriate calibrators. Clin. Chem. 42, 2028-2032 (1996).
23. Araki, A. & Sako, Y. Determination of free and total homocysteine in human plasma by high-performance liquid chromatography with fluorescence detection. J. Chromotogr. 422, 43-52 (1987).
24. Shipchandler, M.T. & Moore, E.G. Rapid, fully automated measurement of plasma homocyst(e)ine with the Abbott IMx analyzer. Clin. Chem. 41, 991-994 (1995).
25. Frantzen, F., Faaren, A.L., Alfheim, I. & Nordhei, A.K. Enzyme conversion immunoassay for determining total homocysteine in plasma or serum. Clin. Chem. 44, 311-316 (1998).
26. Nexo, E. et al. Evaluation of novel assays in clinical chemistry: quantification of plasma total homocysteine. Clin. Chem. 46, 1150-1156 (2000).
27. Pfeiffer, C.M., Huff, D.L., Smith, S.J., Miller, D.T. & Gunter, E.W. Comparison of plasma total homocysteine measurements in 14 laboratories: an international study. Clin. Chem. 45, 1261-1268 (1999).
28. Wang, W., Escobedo, J.O., Lawrence, C.M. & Strongin, R.M. Direct detection of homocysteine. J. Am. Chem. Soc. 126, 3400-3401 (2004).
29. Tan, Y. et al. Total-homocysteine enzymatic assay. Clin. Chem. 46, 1686-1688 (2000).
30. Tripodi, A., Chantarangkul, V., Tan, Y., Hoffman, R.M. & Cattaneo, M. Evaluation of an enzymatic method to measure total homocysteine in plasma. Thromb. Haemost. 87, 172-173 (2002).
31. Tan, Y. et al. Automated enzymatic assay for homocysteine. Clin. Chem. 49, 1029-1030 (2003).
32. NCCLS. Evaluation of Precision Performance of Clinical Chemsitry Devices: Approved Guideline NCCLS document EP5-A (ISBN 1-56238-368-X).
33. Refsum, H. et al. Facts and recommendations about total homocysteine determinations: An expert opinion. Clin. Chem. 50 3-32 (2004).
34. Bland, J.M. & Altman, D.G. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1, 307-310 (1986).
35. Han, Q. et al. High expression, purification, and properties of recombinant homocysteine α, γ-lyase. Protein Expr. Purif. 14 267-274 (1998).