Crystal structure of native O-acetyl-serine sulfhydrylase from Entamoeba histolytica and its complex with cysteine: Structural evidence for cysteine binding and lack of interactions with serine acetyl transferase

Proteins: Structure, Function and Genetics

Volumn 72, Issue 4, 2008, Pages 1222-1232

  Chinthalapudi, Krishna ^a   Kumar, Manish ^a   Kumar, Shivesh ^a   Jain, Shaifali ^a   Alam, Neelima ^a   Gourinath, S ^a  

^a JAWAHARLAL NEHRU UNIVERSITY   (India)

Author keywords

Cysteine synthesis; Entamoeba histolytica; Ligand binding; O acetyl serine sulfhydrylase; Protein protein interactions; Serine acetyl transferase

Indexed keywords

ACETIC ACID DERIVATIVE; CHLORIDE; CYSTEINE; CYSTEINE SYNTHASE; DIMER; O ACETYLSERINE; SERINE ACETYLTRANSFERASE; SERINE DERIVATIVE; SULFIDE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CRYSTAL STRUCTURE; ENTAMOEBA HISTOLYTICA; ENZYME ACTIVE SITE; LIGAND BINDING; MOLECULAR DYNAMICS; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION;

ANIMALS; CATALYTIC DOMAIN; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; CYSTEINE; CYSTEINE SYNTHASE; ENTAMOEBA HISTOLYTICA; PROTEIN BINDING; SERINE O-ACETYLTRANSFERASE;

ENTAMOEBA HISTOLYTICA;

EID: 51349147518     PISSN: 08873585     EISSN: 10970134     Source Type: Journal    
DOI: 10.1002/prot.22013     Document Type: Article

References (35)

1. Byrne CR, Monroe RS, Ward KA, Kredich NM. DNA sequences of the cysK regions of Salmonella typhimurium and Escherichia coli and linkage of the cysK regions to ptsH. J Bacteriol 1998;170:3150-3157.
2. Ogasawara N, Nakai S, Yoshikawa H. Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin. DNA Res 1994;1:1-14.
3. Hell R, Bork C, Bogdanova N, Frolov I, Hauschild R. Isolation and characterization of two cDNAs encoding for compartment specific isoforms of O-acetylserine (thiol) lyase from Arabidopsis thaliana. FEBS Lett 1994;351:57-62.
4. Noji M, Murakoshi I, Saito K. Molecular cloning of a cysteine synthase cDNA from Citrullus vulgaris (watermelon) by genetic complementation in an Escherichia coli Cys-auxotroph. Mol Gen Genet 1994;244:57-66.
5. Romer S, d'Harlingue A, Camara B, Schantz R, Kuntz M. Cysteine synthase from Capsicum annuum chromoplasts. Characterization and cDNA cloning of an up-regulated enzyme during fruit development. J Biol Chem 1992;267:7966-7970.
6. Saito K, Miura N, Yamazaki M, Hirano H, Murakoshi I. Molecular cloning and bacterial expression of cDNA encoding a plant cysteine synthase. Proc Natl Acad Sci USA 1992;89:8078-8082.
7. Noji M, Inoue K, Kimura N, Gouda A, Saito K. Isoform-dependent differences in feedback regulation and subcellular localization of serine acetyltransferase involved in cysteine biosynthesis from Arabidopsis thaliana. J Biol Chem 1998;273:32739-32745.
8. Saito K, Yokoyama H, Noji M, Murakoshi I. Molecular cloning and characterization of a plant serine acetyltransferase playing a regulatory role in cysteine biosynthesis from watermelon. J Biol Chem 1995;270:16321-16321.
9. Droux M, Ruffet ML, Douce R, Job D. Interactions between serine acetyl transferase and O-acetylserine (thiol) lyase in higher plants - structural and kinetic properties of the free and bound enzymes. Eur J Biochem 1998;255:235-245.
10. Mino K, Yamanoue T, Sakiyama T, Eisaki N, Matsuyama A, Nakanishi K. Purification and characterization of serine acetyltransferase from Escherichia coli partially truncated at the C-terminal region. Biosci Biotechnol Biochem 1999;63:168-179.
11. Mino K, Yamanoue T, Sakiyama T, Eisaki N, Matsuyama A, Nakanishi K. Effects of bienzyme complex formation of cysteine synthetase from escherichia coli on some properties and kinetics. Biosci Biotechnol Biochem 2000;64:1628-1640.
12. Huang B, Vetting MW, Roderick SL. The active site of O-acetylserine sulfhydrylase is the anchor point for bienzyme complex formation with serine acetyltransferase. J Bacteriol 2005;187:3201-3205.
13. Francois JA, Kumaran S, Jez JM. Structural basis for interaction of O-acetylserine sulfhydrylase and serine acetyltransferase in the Arabidopsis cysteine synthase complex. Plant Cell 2006;18:3647-3655.
14. Schnell R, Oehlmann W, Singh M, Schneider G. Structural insights into catalysis and inhibition of O-acetylserine sulfhydrylase from mycobacterium tuberculosis: crystal structures of the enzyme {α}-aminoacrylate intermediate and an enzyme-inhibitor complex. J Biol Chem 2007;282:23473-23481.
15. The World Health Report. Bridging the gaps: Report of the Director-General. Geneva: World Health Organization; 1995;16:377-385.
16. Nozaki T, Asai T, Kobayashi S, Ikegami F, Noji M, Saito K, Takeuchi T. Molecular cloning and characterization of the genes encoding two isoforms of cysteine synthase in the enteric protozoan parasite Entamoeba histolytica. Mol Biochem Parasitol 1998;97:33-44.
17. Nozaki T, Asai T, Lidya B S, Seiki K, Miki N, Takeuchi T. Characterization of the gene encoding serine acetyltransferase, a regulated enzyme of cysteine biosynthesis from the protist parasites Entamoeba histolytica and Entamoeba dispar. Regulation and possible function of the cysteine biosynthetic pathway in Entamoeba. J Biol Chem 1999;274:32445-32452.
18. Schnackerz KD, Tai CH, Simmons JW 3rd, Jacobson TM, Rao GS, Cook PF. Identification and spectral characterization of the external aldimine of the O-acetylserine sulfhydrylase reaction. Biochemistry 1995;34:12152-12160.
19. McClure GD, Jr, Cook PF. Product binding to the alpha-carboxyl subsite results in a conformational change at the active site of O-acetylserine sulfhydrylase-A: evidence from fluorescence spectroscopy. Biochemistry 1994;33:1674-1683.
20. Benci S, Vaccari S, Mozzarelli A, Cook PF. Time-resolved fluorescence of O-acetylserine sulfhydrylase. Biochim Biophys Acta 1999;1429:317-330.
21. Burkhard P, Tai CH, Jansonius JN, Cook PF. Identification of an allosteric anion-binding site on O-acetylserine sulfhydrylase: structure of the enzyme with chloride bound. J Mol Biol 2000;303:279-286.
22. Bonner ER, Cahoon RE, Knapke SM, Jez JM. Molecular basis of cysteine biosynthesis in plants: structural and functional analysis of O-acetylserine sulfhydrylase from Arabidopsis thaliana. J Biol Chem 2005;280:38803-38813.
23. Burkhard P, Jagannatha Rao GS, Hohenester E, Schnackerz KD, Cook PF, Jansonius JN. Three-dimensional structure of O-acetylserine sulfhydrylase from Salmonella typhimurium. J Mol Biol 1998;283:121-133.
24. Krishna H, Jain R, Kashav T, Wadhwa D, Alam N, Gourinath S. Crystallization and preliminary crystallographic analysis of cysteine synthase from Entamoeba histolytica. Acta Crystallogr F 2007;63:512-515.
25. Otwinowski Z, Minor W. Processing of X-ray diffraction and data collection in oscillation mode. Methods Eznymol 1997;276:307-326.
26. Perrakis A, Morris RM, Lamzin VS. Automated protein model building combined with iterative structure refinement. Nature Struct Biol 1999;6:458-463.
27. Emsley P, Cowton K. Coot: model-building tools for molecular graphics. Acta Crystallogr D 2004;60:2126-2132.
28. Murshudov GN, Vagin AA, Dodson EJ. Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr D 1997;53:240-255.
29. DeLano WL. The PyMOL users manual. San Carlos, CA, USA: DeLano Scientific; 2002.
30. Vagin A, Teplyakov A. MOLREP: an automated program for molecular replacement. J Appl Crystallogr 1997;30:1022-1025.
31. Jones TA, Zou JY, Cowan SW, Kjeldgaard M. Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallogr A 1991;47:110-119.
32. Brunger AT, Adams PD, Clore GM, DeLano WL, Gros P, Grosse-Kunstleve RW, Jiang JS, Kuszewski J, Nilges N, Pannu NS, Read RJ, Rice LM, Simonson T, Warren GL. Crystallography & NMR system: a new software suite for macromolecular structure determination. Acta Crystallogr D 1998;54:905-921.
33. Tai CH, Burkhard P, Gani D, Jenn T, Johnson C, Cook PF. Characterization of the allosteric anion-binding site of O-acetylserine sulfhydrylase. Biochemistry 2001;40:7446-7452.
34. Burkhard P, Tai CH, Ristroph CM, Cook PF, Jansonius JN. Ligand binding induces a large conformational change in O-acetylserine sulfhydrylase from Salmonella typhimurium. J Mol Biol 1999;291:941-953.
35. Claus MT, Zocher GE, Maier TH, Schulz GF. Structure of the O-acetylserine sulfhydrylase isoenzyme CysM from Escherichia coli. Biochemistry 2005;44:8620-8626.