Crystal structure of a self-assembling lipopeptide detergent at 1.20 Å

Proceedings of the National Academy of Sciences of the United States of America

Volumn 105, Issue 35, 2008, Pages 12861-12866

  Ho, Dona N ^a   Pomroy, Neil C ^a   Cuesta Seijo, José A ^a   Privé, Gilbert G ^a,b  

^a PRINCESS MARGARET HOSPITAL   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

De novo protein design; Detergent design; Membrane proteins; Self assembling amphiphiles; X ray crystallography

Indexed keywords

CARBENE; DODECYLMALTOSIDE; LAURIC ACID; LIPOPEPTIDE; MALTOSE; METHYL GROUP; MONOMER; UNCLASSIFIED DRUG;

ARTICLE; CRYSTAL STRUCTURE; CRYSTALLIZATION; HYDROPHILICITY; MICELLE; MOLECULAR WEIGHT; PRIORITY JOURNAL; PROTEIN CONFORMATION; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; CRYSTALLOGRAPHY, X-RAY; DETERGENTS; DIMERIZATION; GLUCOSIDES; HYDROPHOBICITY; LIPIDS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEIN STRUCTURE, SECONDARY;

EID: 51349125801     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0801941105     Document Type: Article

References (25)

1. le Maire M, Champeil P, Moller JV (2000) Interaction of membrane proteins and lipids with solubilizing detergents. Biochim Biophys Acta 1508:86-111.
2. Garavito RM, Ferguson-Miller S (2001) Detergents as tools in membrane biochemistry. J Biol Chem 276:32403-32406.
3. Page RC, et al. (2006) Comprehensive evaluation of solution nuclear magnetic resonance spectroscopy sample preparation for helical integral membrane proteins. J Struct Funct Genomics 7:51-64.
4. Privé GG (2007) Detergents for the stabilization and crystallization of membrane proteins. Methods 41:388-397.
5. Wiener MC (2004) A pedestrian guide to membrane protein crystallization. Methods 34:364-372.
6. Patargias G, Bond PJ, Deol SS, Sansom MS (2005) Molecular dynamics simulations of GlpF in a micelle vs in a bilayer: Conformational dynamics of a membrane protein as a function of environment. J Phys Chem B 109:575-582.
7. McGregor CL, et al. (2003) Lipopeptide detergents designed for the structural study of membrane proteins. Nat Biotechnol 21:171-176.
8. Schafmeister CE, Miercke LJ, Stroud RM (1993) Structure at 2.5 Å of a designed peptide that maintains solubility of membrane proteins. Science 262:734-738.
9. Kelly E, Privé GG, Tieleman DP (2005) Molecular models of lipopeptide detergents: Large coiled-coils with hydrocarbon interiors. J Am Chem Soc 127:13446-13447.
10. Harbury PB, Kim PS, Alber T (1994) Crystal structure of an isoleucine-zipper trimer. Nature 371:80-83.
11. Gernert KM, Surles MC, Labean TH, Richardson JS, Richardson DC (1995) The Alacoil: A very tight, antiparallel coiled-coil of helices. Protein Sci 4:2252-2260.
12. Ogihara NL, et al. (2001) Design of three-dimensional domain-swapped dimers and fibrous oligomers. Proc Natl Acad Sci USA 98:1404-1409.
13. Ghirlanda G, Lear JD, Ogihara NL, Eisenberg D, DeGrado WF (2002) A hierarchic approach to the design of hexameric helical barrels. J Mol Biol 319:243-253.
14. Deng Y, et al. (2006) Antiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeat. Structure 14:247-255.
15. Liu J, et al. (2007) Conformational specificity of the lac repressor coiled-coil tetramerization domain. Biochemistry 46:14951-14959.
16. Geremia S, et al. (2005) Response of a designed metalloprotein to changes in metal ion coordination, exogenous ligands, and active site volume determined by x-ray crystallography. J Am Chem Soc 127:17266-17276.
17. Qin L, Hiser C, Mulichak A, Garavito RM, Ferguson-Miller S (2006) Identification of conserved lipid/detergent-binding sites in a high-resolution structure of the membrane protein cytochrome c oxidase. Proc Natl Acad Sci USA 103:16117-16122.
18. North B, Summa CM, Ghirlanda G, DeGrado WF (2001) D(n)-symmetrical tertiary templates for the design of tubular proteins. J Mol Biol 311:1081-1090.
19. DeGrado WF, Summa CM, Pavone V, Nastri F, Lombardi A (1999) De novo design and structural characterization of proteins and metalloproteins. Annu Rev Biochem 68:779-819.
20. Miyatake H, Hasegawa T, Yamano A (2006) New methods to prepare iodinated derivatives by vaporizing iodine labelling (VIL) and hydrogen peroxide VIL (HYPERVIL). Acta Crystallogr D Biol Crystallogr 62:280-289.
21. Minor W, Cymborowski M, Otwinowski Z, Chruszcz M (2006) HKL-3000: The integration of data reduction and structure solution-from diffraction images to an initial model in minutes. Acta Crystallogr D Biol Crystallogr 62:859-866.
22. Terwilliger TC (2003) SOLVE and RESOLVE: Automated structure solution and density modification. Methods Enzymol 374:22-37.
23. Murshudov GN, Vagin AA, Dodson EJ (1997) Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr D Biol Crystallogr 53:240-255.
24. Sheldrick GM (2008) A short history of SHELX. Acta Crystallogr A 64:112-122.
25. Emsley P, Cowtan K (2004) Coot: Model-Building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr 60:2126-2132.