DNA quantification and fragmentation in sputum after inhalation of recombinant human deoxyribonuclease

Cellular Physiology and Biochemistry

Volumn 22, Issue 1-4, 2008, Pages 347-352

  Riethmueller, Joachim ^a   Vonthein, Reinhard ^c   Borth Bruhns, Thomas ^a   Grassmé, Heike ^b   Eyrich, Matthias ^a   Schilbach, Karin ^a   Stern, Martin ^a   Gulbins, Erich ^b  

^a UNIVERSITY HOSPITAL TÜBINGEN   (Germany)

^b UNIVERSITY OF DUISBURG ESSEN   (Germany)

^c UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

Cystic fibrosis; Deoxyribonucleic acid; Fragmentation; Recombinant human deoxyribonuclease; Sputum

Indexed keywords

DORNASE ALFA;

AGAR GEL ELECTROPHORESIS; ARTICLE; CONTROLLED STUDY; DENSITOMETRY; DNA CLEAVAGE; DNA CONTENT; DNA DETERMINATION; DNA FRAGMENTATION; HUMAN; LUNG FIBROSIS; MAJOR CLINICAL STUDY; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; QUANTITATIVE ANALYSIS; SPUTUM ANALYSIS; T LYMPHOCYTE;

PSEUDOMONAS AERUGINOSA;

EID: 51149090177     PISSN: 10158987     EISSN: None     Source Type: Journal    
DOI: 10.1159/000149813     Document Type: Article

References (27)

1. Aitken ML, Burke W, McDonald G, Shak S, Montgomery AB, Smith A: Recombinant human DNase inhalation in normal subjects and patients with cystic fibrosis. A phase I study. Jama 1992;267:1947-51.
2. Harms HK, Matouk E, Tournier G, von der Hardt H, Weller PH, Romano L, Heijerman HG, FitzGerald MX, Richard D, Strandvik B, Kolbe J, Kraemer R, Michalsen H: Multicenter, open-label study of recombinant human DNase in cystic fibrosis patients with moderate lung disease. DNase International Study Group. Pediatr Pulmonol 1998;26:155-61.
3. Shak S, Capon DJ, Hellmiss R: Marsters, S.A. & Baker, C.L. Recombinant human DNase I reduces the viscosity of cystic fibrosis sputum. Proc Natl Acad Sci U S A 1990;87:9188-92.
4. Aitken ML: Clinical trials of recombinant human DNase in cystic fibrosis patients. Monaldi Arch Chest Dis 1993;48:653-6.
5. Dasgupta B, King M: Reduction in viscoelasticity in cystic fibrosis sputum in vitro using combined treatment with nacystelyn and rhDNase. Pediatr Pulmonol 1996;22:161-6.
6. Kirchner KK, Wagener JS, Khan TZ, Copenhaver SC, Accurso FJ: Increased DNA levels in bronchoalveolar lavage fluid obtained from infants with cystic fibrosis. Am J Respir Crit Care Med 1996;154:1426-9.
7. Shah PL, Scott SF, Knight RA, Marriott C, Ranasinha C, Hodson ME: In vivo effects of recombinant human DNase I on sputum in patients with cystic fibrosis. Thorax 1996;51:119-25.
8. King M, Dasgupta B, Tomkiewicz RP, Brown NE: Rheology of cystic fibrosis sputum after in vitro treatment with hypertonic saline alone and in combination with recombinant human deoxyribonuclease I. Am J Respir Crit Care Med 1997;156:173-7.
9. Desai M, Weller PH, Spencer DA: Clinical benefit from nebulized human recombinant DNase in Kartagener's syndrome. Pediatr Pulmonol 1995;20:307-8.
10. Bates RD, Nahata MC: Aerosolized dornase alpha (rhDNase) in cystic fibrosis. J Clin Pharm Ther 1995;20:313-5.
11. Paul K, Rietschel E, Ballmann M, Griese M, Worlitzsch D, Shute J, Chen C, Schink T, Döring G, van Koningsbruggen S, Wahn U, Ratjen F: Effect of treatment with dornase alpha on airway inflammation in patients with cystic fibrosis. Am J Respir Crit Care Med 2004;169:719-25.
12. Shah PL, Scott SF, Knight RA, Hodson ME: The effects of recombinant human DNase on neutrophil elastase activity and interleukin-8 levels in the sputum of patients with cystic fibrosis. Eur Respir 1996;9:531-4.
13. Ratjen F, Paul K, van Koningsbruggen S, Breitenstein S, Rietschel E, Nikolaizik W: DNA concentrations in BAL fluid of cystic fibrosis patients with early lung disease: influence of treatment with dornase alpha. Pediatr Pulmonol 2005;39:1-4.
14. Barker M, Franke E, Bohle M, Pfannenstiel C, Heimann G: Effect of DNase on exercise capacity in cystic fibrosis. Pediatr Pulmonol 2004;38:70-4.
15. Fuchs HJ, Borowitz DS, Christiansen DH, Morris EM, Nash ML, Ramsey BW, Rosenstein BJ, Smith AL, Wohl ME: Effect of aerosolized recombinant human DNase on exacerbations of respiratory symptoms and on pulmonary function in patients with cystic fibrosis. The Pulmozyme Study Group. N Engl J Med 1994;331:637-42.
16. Durward A, Forte V, Shemie SD: Resolution of mucus plugging and atelectasis after intratracheal rhDNase therapy in a mechanically ventilated child with refractory status asthmaticus. Crit Care Med 2000;28:560-2.
17. Riethmueller J, Borth-Bruhns T, Kumpf M, Vonthein R, Wiskirchen J, Stern M, Hofbeck M, Baden W: Recombinant Human Deoxyribonuclease Shortens Ventilation Time in Young, Mechanically Ventilated Children. Pediatr Pulmonol 2006;41:61-66.
18. Touleimat BA, Conoscenti CS, Fine JM: Recombinant human DNase in management of lobar atelectasis due to retained secretions. Thorax 1995;50:1319-21.discussion 1323.
19. Voelker KG, Chetty KG, Mahutte CK: Resolution of recurrent atelectasis in spinal cord injury patients with administration of recombinant human DNase. Intensive Care Med 1996;22:582-4.
20. Puterman AS, Weinberg EG: rhDNase in acute asthma. Pediatr Pulmonol 1997;23:316-7.
21. Wills PJ, Wodehouse T, Corkery K, Mallon K, Wilson R, Cole PJ: Short-term recombinant human DNase in bronchiectasis. Effect on clinical state and in vitro sputum transportability. Am J Respir Crit Care Med 1996;154:413-7.
22. Daugherty AL, Patapoff TW, Clark RC, Sinicropi DV, Mrsny RJ: Compaction assay: a rapid and simple in vitro method to assess the responsiveness of a biopolymer matrix to enzymatic modification. Biomaterials 1995;16:553-8.
23. Brandt T, Breitenstein S, von der Hardt H, Tummler B: DNA concentration and length in sputum of patients with cystic fibrosis during inhalation with recombinant human DNase. Thorax 1995;50:880-2.
24. Burton K: A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 1956;62:315-23.
25. Croft DN, Lubran M: The estimation of DNA in the presence of sialic acid: application to analysis of human gastric washing. Biochem J 1965;95:612-20.
26. Sagel SD, Sontag MK, Wagener JS, Kapsner RK, Osberg I, Accurso FJ: Induced sputum inflammatory measures correlate with lung function in children with cystic fibrosis. J Pediatr 2002;141:811-817.
27. Suri R, Marshall LJ, Wallis C, Metcalfe C, Bush A, Shute JK: Effects of recombinant human DNase and hypertonic saline on airway inflammation in children with cystic fibrosis. Am J Respir Crit Care Med 2002;166:352-5.