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Chemistry Letters
Volumn 37, Issue 8, 2008, Pages 876-877
Synthesis of oligosaccharides of genistein and quercetin as potential anti-inflammatory agents
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EID: 51149084742     PISSN: 03667022     EISSN: None     Source Type: Journal    
DOI: 10.1246/cl.2008.876     Document Type: Article
Times cited : (20)
References (13)
  • 8
    • 51149101156 scopus 로고    scopus 로고
    • Typical procedure of glycosylation with cultured plant cells is as follows. A total of 1 mmol of genistein (1) was administered to ten 300-mL conical flasks (0.1 mmol/flask) containing 70 g of suspension cultured I. batatas cells, and the cultures were incubated at 25 °C for 5 days on a rotary shaker (120 rpm, After the incubation period, the cells and medium were separated by filtration with suction. The cells were extracted (x3) by homogenization with MeOH and the extract was concentrated. The residue was partitioned between H2O and EtOAc. The H2O layer was applied to a Diaion HP-20 column, and the column was washed with H2O followed by elution with MeOH. The MeOH eluate was subjected to HPLC [column: YMC-Pack R&D ODS column (150 x 30mm, solvent: MeOH-H2O (9:11, v/v, detection: UV 280nm, flow rate: 1.0 mL/min] to give products
    • 2O (9:11, v/v); detection: UV (280nm); flow rate: 1.0 mL/min] to give products.
  • 9
    • 51149118810 scopus 로고    scopus 로고
    • Typical procedure of CGTase-catalyzed glycosylation is as follows. To a solution containing 10 mL of 25 mM sodium phosphate buffer (pH 7.0), 0.2 mmol of genistein 4′-O-β-D-glucoside (2), and 5 g of soluble starch was added 300 units of CGTase (Amano Pharmaceutical Co., Ltd). The mixture was incubated for 24 h at 40°C. After centrifugation of the mixture at 3000 g for 10 min, the supernatant was subjected to Sephadex G-25 column chromatography. The glycoside fractions were lyophilized, and subjected to purification by preparative HPLC.
    • Typical procedure of CGTase-catalyzed glycosylation is as follows. To a solution containing 10 mL of 25 mM sodium phosphate buffer (pH 7.0), 0.2 mmol of genistein 4′-O-β-D-glucoside (2), and 5 g of soluble starch was added 300 units of CGTase (Amano Pharmaceutical Co., Ltd). The mixture was incubated for 24 h at 40°C. After centrifugation of the mixture at 3000 g for 10 min, the supernatant was subjected to Sephadex G-25 column chromatography. The glycoside fractions were lyophilized, and subjected to purification by preparative HPLC.
  • 10
    • 51149085546 scopus 로고    scopus 로고
    • Each compound was stirred in water for 24 h at 25 °C. The mixture was centrifuged at 100000g for 30min at 25°C. The concentration of test compounds was estimated on the basis of their peak areas using calibration curves prepared by HPLC analyses of authentic samples.
    • Each compound was stirred in water for 24 h at 25 °C. The mixture was centrifuged at 100000g for 30min at 25°C. The concentration of test compounds was estimated on the basis of their peak areas using calibration curves prepared by HPLC analyses of authentic samples.
  • 11
    • 51149119011 scopus 로고    scopus 로고
    • Peritoneal mast cells were collected from the abdominal cavity of rats (Male Wistar rats, Nippon SLC) and purified to a level higher than 95% according to a method previously described.8 The purified mast cells were suspended in a physiological buffered solution (PBS) containing 145mM NaCl, 2.7mM KCl, 1.0 mM CaCl2, 5.6mM glucose, and 20 mM HEPES (pH 7.4) to give approximately 104 mast cells/mL. Cell viability was always greater than 90% as judged by the trypan blue exclusion test. Mast cells were preincubated with the test compound (1 μM) for 15 min at 37 °C, and subsequently exposed to compound 48/80 at 0.35 μg/mL. Histamine release was determined by a fluorometric assay according to the previously reported method, and was expressed as a percentage of total histamine.8
    • 8
  • 13
    • 51149095524 scopus 로고    scopus 로고
    • Supporting Information is available electronically on the CSJ-Journal Web site, http://www.csj.jp/journals/chem-lett/index.html.
    • Supporting Information is available electronically on the CSJ-Journal Web site, http://www.csj.jp/journals/chem-lett/index.html.

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.