PHEMA hydrogels modified through the grafting of phosphate groups by ATRP support the attachment and growth of human corneal epithelial cells

Journal of Biomaterials Applications

Volumn 23, Issue 2, 2008, Pages 147-168

  Zainuddin, Z ^a   Barnard, Zeke ^a   Keen, Imelda ^b   Hill, David J T ^b   Chirila, Traian V ^a,b,c   Harkin, Damien G ^c  

^a QUEENSLAND EYE INSTITUTE   (Australia)

^b UNIVERSITY OF QUEENSLAND   (Australia)

^c QUEENSLAND UNIVERSITY OF TECHNOLOGY   (Australia)

Author keywords

ATRP grafting; Corneal epithelial cells; Hydrogels biomaterials; Phosphate groups; Tissue engineering

Indexed keywords

ABS RESINS; CELL CULTURE; CHEMICAL REACTIONS; COLLOIDS; COMPUTER NETWORKS; COPPER; CYTOLOGY; FREE RADICAL REACTIONS; GRAFTING (CHEMICAL); HYDROGELS; INFRARED SPECTROSCOPY; METHANOL; MOLECULAR ORBITALS; MOLECULAR SPECTROSCOPY; MONOMERS; NANOSTRUCTURED MATERIALS; PHOTOELECTRON SPECTROSCOPY; PLASTICS; POLYMERS; TISSUE CULTURE; X RAY PHOTOELECTRON SPECTROSCOPY;

ATRP GRAFTING; CORNEAL EPITHELIAL CELLS; HYDROGELS BIOMATERIALS; PHOSPHATE GROUPS; TISSUE ENGINEERING;

ATOM TRANSFER RADICAL POLYMERIZATION;

BIOMATERIAL; CROSS LINKING REAGENT; METHACRYLIC ACID DERIVATIVE; MONO(2 METHACRYLOYLOXY)ETHYL PHTHALATE; MONO(2-METHACRYLOYLOXY)ETHYL PHTHALATE; PHOSPHATE; PHTHALIC ACID DERIVATIVE; POLYMACON; UNCLASSIFIED DRUG;

ARTICLE; CELL ADHESION; CELL CULTURE; CELL PROLIFERATION; CHEMISTRY; CORNEA EPITHELIUM; CULTURE TECHNIQUE; CYTOLOGY; EPITHELIUM CELL; EVALUATION; HUMAN; MATERIALS TESTING; METHODOLOGY; PHYSIOLOGY; TISSUE ENGINEERING;

BIOCOMPATIBLE MATERIALS; CELL ADHESION; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CELLS, CULTURED; CROSS-LINKING REAGENTS; EPITHELIAL CELLS; EPITHELIUM, CORNEAL; HUMANS; MATERIALS TESTING; METHACRYLATES; PHOSPHATES; PHTHALIC ACIDS; POLYHYDROXYETHYL METHACRYLATE; TISSUE ENGINEERING;

EID: 51049116642     PISSN: 08853282     EISSN: 15308022     Source Type: Journal    
DOI: 10.1177/0885328207086993     Document Type: Article

References (86)

1. Drury, J.L. and Mooney, D.J. (2003). Hydrogels for Tissue Engineering: Scaffold Design Variables and Applications, Biomaterials, 24: 4337-4351.
2. Wichterle, O. and Lim, D. (1960). Hydrophilic Gels for Biological Use, Nature, 185: 117-118.
3. Ruben, M. (1978). Soft Contact Lenses, Clinical and Applied Technology, Wiley-VCH, New York.
4. Singh, J. and Agrawal, K.K. (1992). Polymeric Materials for Contact Lenses, Rev. Macromol. Chem. Phys., C32: 521-534.
5. Barrett, G.D., Constable, I.J. and Stewart, A.D. (1986). Clinical-results of Hydrogel Lens Implantation, J. Cataract Refract. Surg., 12: 623-631.
6. Menapace, R., Skorpik, C., Juchem, M., Schneidel, W. and Schranz, R. (1989). Evaluation of the First 60 Cases of PHEMA Posterior Chamber Lenses Implanted in the Sulcus, J. Cataract Refract. Surg., 15: 264-271.
7. Chirila, T.V. (2001). An Overview of the Development of Artificial Corneas with Porous Skirts and the Use of PHEMA for Such an Application, Biomaterials, 22: 3311-3317.
8. Hicks, C.R., Crawford, G.J., Lou, X., Tan, D., Snibson, G.R., Sutton, G., Downie, N., Werner, L., Chirila, T.V. and Constable, I.J. (2003). Corneal Replacement Using a Synthetic Hydrogel Cornea, AlphaCor(TM): Device, Preliminary Outcomes and Complications, Eye, 17: 385-392.
9. Ratner, B.D. and Hoffman, A.S. (1976). Synthetic Hydrogels for Biomedical Applications, ACS Symp. Ser., 31: 1-36.
10. Teijon, J.M., Trigo, R.M., Garcia, O. and Blanco, M.D. (1997). Cytarabine Trapping in Poly(2-hydroxyethyl methacrylate) Hydrogels: Drug Delivery Studies, Biomaterials, 18: 383-388.
11. Lu, S. and Anseth, K.S. (1999). Photopolymerization of Multi-laminated Poly(HEMA) Hydrogels for Controlled Release, J. Contr. Rel., 57: 291-300.
12. Ratner, B.D., Horbett, T., Hoffman, A.S. and Hauschka, S.D. (1975). Cell Adhesion to Polymeric Materials: Implications with Respect to Biocompatibility, J. Biomed. Mater. Res., 9: 407-422.
13. Horbett, T.A., Schway, M.B. and Ratner, B.D. (1985). Hydrophilichydrophobic Copolymers as Cell Substrates-effect on 3T3 Cell-growth Rates, J. Coilloid Interface Sci., 104: 28-39.
14. Horbett, T.A., Waldburger, J.J., Ratner, B.D. and Hoffman, A.S. (1988). Cell Adhesion to a Series of Hydrophilic-hydrophobic Copolymers Studies with a Spinning Disc Apparatus, J. Biomed. Mater. Res., 22: 383-404.
15. Folkman, J. and Moscona, A. (1978). Role of Cell Shape in Growth Control, Nature 273: 345-349.
16. Minett, T.W., Tighe, B.J., Lydon, M.J. and Rees, D.A. (1984). Requirements for Cell Spreading on PolyHEMA Coated Culture Substrates, Cell Biol. Int. Rep., 8: 151-159.
17. Lydon, M.J., Minett, T.W. and Tighe, B.J. (1985). Cellular Interactions with Synthetic Polymer Surfaces in Culture, Biomaterials, 6: 396-402.
18. Lydon, M.J. (1986). Synthetic Hydrogels as Substrata for Cell Adhesion Studies, Br. Polym. J., 18: 22-27.
19. Huet, C., Pisselet, C., Mandon-Pépin, B., Monget, P. and Monniaux, D. (2001). Extracellular Matrix Regulates Ovine Granulosa Cell Survival, Proliferation and Steroidogenesis: Relationships between Cell Shape and Function, J. Endocrinol., 169: 347-360.
20. Ayhan, H., Kozluca, A., Pişkin, E. and Gürhan, I. (1999). Attachment of 3T3 and MDBK Cells onto PHEMA-based Microbeads and Their Biologically Modified Forms, J. Bioact. Compat. Polym., 14: 17-30.
21. Lombello, C.B., Santos Jr, A.R., Malmonge, S.M., Barbanti, S.H., Wada, M.L.F. and Duek, E.A.R. (2002). Adhesion and Morphology of Fibroblastic Cells Cultured on Different Polymeric Biomaterials J. Mater. Sci. Mater. Med., 13: 867-874.
22. Massia, S.P. and Hubbell, J.A. (1990). Covalently Attached GRGD on Polymer Surfaces Promotes Biospecific Adhesion of Mammalian Cells, Ann. N. Y. Acad. Sci., 589: 261-270.
23. Denizli, A., Pişkin, E., Dixit, V., Arthur, M. and Gitnick, G. (1995). Collagen and Fibronection Immobilization on PHEMA Microcarriers for Hepatocyte Attachments, Int. J. Artif. Organs, 18: 90-95.
24. Ayhan, H., Pişkin, E. and Gürhan, I. (1999). Cell Growth on Poly(EGDMA/HEMA) Based Microbeads, J. Bioact. Compat. Polym., 14: 399-414.
25. Merret, K., Griffith, C.M., Deslandes, Y., Pleizier, G. and Sheardown, H. (2001). Adhesion of Corneal Epithelial Cells to Cell Adhesion Peptide Modified PHEMA Surfaces, J. Biomater. Sci. Polym. Edn., 12: 647 - 671.
26. Mei, Y., Beers, K.L., Byrd, H.C.M., VanderHart, D.L. and Washburn, N.R. (2004). Solid-phase ATRP Synthesis of Peptide-polymer Hybrids, J. Am. Chem. Soc., 126: 3472-3476.
27. Jacob, J.T., Rochefort, J.R., Bi, J. and Gebhardt, B.M. (2005). Corneal Epithelial Cell Growth over Tethered-protein/peptide Surface-modified Hydrogels, J. Biomed. Mater. Res., Part B: Appl. Biomater., 72B: 198-205.
28. Tugulu, S., Silacci, P., Stergiopulos, N. and Klok, H.-A. (2007). RGDfunctionalized Polymer Brushes as Substrates for the Integrin Specific Adhesion of Human Umbilical Vein Endothelial Cells, Biomaterials, 28: 2536-2546.
29. Kapur, T.A. and Shoichet, M.S. (2003). Chemically-bound Nerve Growth Factor for Neural Tissue Engineering Applications, J. Biomater. Sci. Polym. Edn., 14: 383-394.
30. Moore, K., Macsween, M. and Shoichet, M. (2006). Immobilized Concentration Gradients of Neurotrophic Factors Guide Neurite Outgrowth of Primary Neurons in Macroporous Scaffolds, Tissue Eng., 12: 267-278.
31. Hattori, S., Andrade, J.D., Hibbs, J.B., Gregonis, D.E. and King, R.N. (1985). Fibroblast Cell Prolifiration on Charged Hydroxyethyl Methacrylate Copolymers, J. Colloid and Interface Sci., 104: 72-78.
32. Van Wachem, P.B., Hogt, A.H., Beugeling, T., Feijen, J., Bantjes, A., Detmers, J.P. and Van Aken, W.G. (1987). Adhesion of Cultured Human Endothelial Cells onto Methacrylate Polymers with Varying Surface Wettability and Charge, Biomaterials, 8: 323-328.
33. Pokharna, H.K., Zhong, Y., Smith, D.J. and Dunphy, M. (1990). Copolymers of Hydroxyethyl Methacrylate with Quadrol Methacrylate and with Various Aminoalkyl Methacrylamides as Fibroblast Cell Subtrata, J. Bioact. Compat. Polym., 5: 42-52.
34. Kiremitçi, M., Peşmen, A., Pulat, M. and Gürhan, I. (1993). Relationship of Surface Characteristics to Cellular Attachment in PU and PHEMA, J. Biomater. Appl., 7: 250-264.
35. Burmeister, J.S., Vrany, J.D., Reichert, W.M. and Truskey, G.A. (1996). Effect of Fibronectin Amount and Conformation on the Strength of Endothelial Cell Adhesion to HEMA/EMA Copolymers, J. Biomed. Mater. Res., 30: 13-22.
36. Peluso, G., Petillo, O., Anderson, J.M., Ambrosio, L., Nicolais, L., Melone, M.A.B., Eschbach, F.O. and Huang, S.J. (1997). The Differential Effects of Poly(2-hydroxyethyl methacrylate) and Poly(2-hydroxyethyl methacrylate)/poly (caprolactone) Polymers on Cell Proliferation and Collagen Synthesis by Human Lung Fibroblasts, J. Biomed. Mater. Res., 34: 327-336.
37. Lukas, J., Smetana Jr, K., Petrovicky, P., Palečkova, V., Vačik, J., Dvorankova, B., Broz, L., Pospisilova, D., Holikova, Z. and Bartunkova, J. (2001). Biological Properties of Copolymer of 2-Hydroxyethyl Methacrylate with Sulfopropyl Methacrylate, J. Mater. Sci. Mater. Med., 12: 639-646.
38. Karakečili, A.G. and Gümüşderelioǧlu, M. (2002). Comparison of Bacterial and Tissue Cell Initial Adhesion on Hydrophilic/hydrophobic Biomaterials, J. Biomater. Sci. Polym. Edn., 13: 185-196.
39. Schneider, G.B., English, A., Abraham, M., Zaharias, R., Stanford, C. and Keller, J. (2004). The Effect of Hydrogel Charge Density on Cell Attachment, Biomaterials, 25: 3023-3028.
40. McAuslan, B.R. and Johnson, G. (1987). Cell Responses to Biomaterials I: Adhesion and Growth of Vascular Endothelial Cells on Poly(hydroxyethyl methacrylate) Following Surface Modification by Hydrolytic Etching, J. Biomed. Mater. Res., 21: 921-935.
41. Sipehia, R., Garfinkle, A., Jackson, W.B. and Chang, T.M.S. (1990). Towards an Artificial Cornea-surface Modifications of Optically Clear, Oxygen Permeable Soft Contact-lens Materials by Ammonia Plasma Modification Technique for Enhanced Attachment and Growth of Corneal Epithelian-cells, Biomater. Artif. Cells. Artif. Organs, 18: 643-655.
42. Schroder, K., Meyer-Plath, A., Keller, D. and Ohl, A. (2002). On the Applicability of Plasma Assisted Chemical Micropatterning of Different Polymeric Biomaterials, Plasma and Polym., 7: 103-125.
43. McArthur, S.L., Halter, M.W., Vogel, V. and Castner, D.G. (2003), Covalent Coupling and Characterization of Supported Lipid Layers, Langmuir, 19: 8316-8324.
44. Schiraldi, C., D'Agostino, A., Oliva, A., Flamma, F., De Rosa, A., Apicella, A., Aversa, R. and De Rosa, M. (2004). Development of Hybrid Materials Based on Hydroxyethylmethacrylate as Supports for Improving Cell Adhesion and Proliferation, Biomaterials, 25: 3645-3653.
45. Mei, Y., Wu, T., Xu, C., Langenbach, K.J., Elliott, J.T., Vogt, B.D., Beers, K.L., Amis, E.J. and Washburn, N.R. (2005). Tuning Cell Adhesion on Gradient Poly(2-hydroxyethyl methacrylate)-grafted Surfaces, Langmuir, 21: 12309-12314.
46. Bhat, R.R., Chaney, B.N., Rowley, J., Liebmann-Vinson, A. and Genzer, J. (2005). Tailoring Cell Adhesion Using Surface-grafted Polymer Gradient Assemblies, Adv. Mater., 17: 2802-2807.
47. Yoshikawa, C., Goto, A., Tsujii, Y., Fukuda, T., Kimura, T., Yamamoto, K. and Kishida, A. (2006). Protein Repellency of Well-defined, Concentrated Poly(2-hydroxyethyl methacrylate) Brushes by the Size-exclusion Effect, Macromolecules, 39: 2284-2290.
48. Zeng, F., Shen, Y., Zhu, S. and Pelton, R. (2000). Atom Transfer Radical Polymerization of 2-(Dimethylamino)ethyl Methacrylate in Aqueous Media, J. Polym. Sci. Part A: Polym. Chem., 38: 3821-3827.
49. Wang, X.S., Jackson, R.A. and Armes, S.P. (2000). Facile Synthesis of Acidic Copolymers via Atom Transfer Radical Polymerization in Aqueous Media at Ambient Temperature, Macromolecules, 33: 255-257.
50. Wang, X.S. and Armes, S.P. (2000). Facile Atom Transfer Radical Polymerization of Methoxy-capped Oligo(ethylene glycol) Methacrylate in Aqueous Media at Ambient Temperature, Macromolecules, 33: 6640-6647.
51. Robinson, K.L., Khan, M.A., de Paz Banez, M.V., Wang, X.S. and Armes, S.P. (2001). Controlled Polymerization of 2-Hydroxyethyl Methacrylate by ATRP at Ambient Temperature, Macromolecules, 34: 3155-3158.
52. Save, M., Weaver, J.V.M., Armes, S.P. and McKenna, P. (2002). Atom Transfer Radical Polymerization of Hydroxyl-functional Methacrylates at Ambient Temperature: Comparison of Glycerol Monomethacrylate with 2-Hydroxypropyl Methacrylate, Macromolecules, 35: 1152-1159.
53. Jin, X., Shen, Y. and Zhu, S. (2003). Atom Transfer Radical Block Copolymerization of 2-(N,N-Dimethylamino)ethyl Methacrylate and 2-Hydroxyethyl Methacrylate, Macromol. Mater. Eng., 288: 925-935.
54. Weaver, J.V.M., Bannister, I., Robinson, K.L., Bories-Azeau, X., Armes, S.P., Smallridge, M. and McKenna, P. (2004). Stimulus Responsive Water Soluble Polymers Based on 2-Hydroxyethyl Methacrylate, Macromolecules, 37: 2395-2403.
55. Chung, I., Britt, P., Xie, D., Harth, E. and Mays, J. (2005). Synthesis of Amino Acid-based Polymers via Atom Transfer Radical Polymerization in Aqueous Media at Ambient Temperature, Chem. Comm., 8: 1046-1048.
56. Ziegelaar, B.W., Fitton, J.H., Clayton, A.B., Platten, S.T. and Chirila, T.V. (1998). The Modulation of Cellular Responses to Poly(2-hydroxyethyl methacrylate) Hydrogel Surfaces: Phosphorylation Decreases Macrophage Collagenase Production in Vitro, J. Biomater. Sci. Polym. Edn., 9: 849-862.
57. Ziegelaar, B.W., Fitton, J.H., Clayton, A.B., Platten, S.T., Maley, M.A.L. and Chirila, T.V. (1999). The Modulation of Corneal Keratocyte and Epithelial Cell Responses to Poly(2-hydroxyethyl methacrylate) Hydrogel Surfaces: Phosphorylation Decreases Collagenase Production, Biomaterials, 20: 1979-1988.
58. Nuttelman, C.R., Benoit, D.S.W., Tripodi, M.C. and Anseth, K.S. (2006). The Effect of Ethylene Glycol Methacrylate Phosphate in PEG Hydrogels on Mineralization and Viability of Encapsulated hMSCs, Biomaterials, 27: 1377-1386.
59. Tan, J., Gemeinhart, R.A., Ma, M. and Saltzman, W.M. (2005). Improved Cell Adhesion and Proliferation on Synthetic Phosphonic Acid-containing Hydrogels, Biomaterials, 26: 3663-3671.
60. Barnard, Z., Apel, A.J. and Harkin, D.G. (2001). Phenotypic Analyses of Limbal Epithelial Cell Cultures Derived from Donor Corneoscleral Rims, Clin. Expt. Ophthalmol., 29: 138-142.
61. Haddleton, D.M., Crossman, M.C., Dana, B.H., Duncalf, D.J., Heming, A.M., Kukulj, D. and Shooter, A.J. (1999). Atom Transfer Polymerization of Methyl Methacrylate Mediated by Alkylpyridylmethanimine Type Ligands, Copper(I) Bromide, and Alkyl Halides in Hydrocarbon Solution, Macromolecules, 32: 2110-2119.
62. Matyjaszewski, K. and Xia, J. (2001). Atom Transfer Radical Polymerization, Chem. Rev., 101: 2921-2990.
63. Percec, V., Guliashvili, T., Ladislaw, J.S., Wistrand, A., Stjerndahl, A., Sienkowska, M.J., Monteiro, M.J. and Sahoo, S. (2006). Ultrafast Synthesis of Ultrahigh Molar Mass Polymers by Metal-catalyzed Living Radical Polymerization of Acrylates, Methacrylates, and Vinyl Chloride Mediated by SET at 25 Degrees C, J. Am. Chem. Soc., 128: 14156-14165.
64. Harvey, M.C. and Ketley, A.D. (1961). The Infrared Identification of Short-chain Branches in Polyolefins, J. Appl. Polym. Sci., 5: 247-250.
65. Coates, J. (2000). A Practical Approach to the Interpretation of Infrared Spectra, Meyers, R.A. (ed.), In: Encyclopedia of Analytical Chemistry, John Wiley & Sons Ltd., Chechester, UK.
66. Weiss, R.A., Lenz, R.W. and Macknight, W.J. (1977). Properties of Polyethylene Modified with Phosphonate Side Groups I. Thermal and Mechanical Properties, J. Polym. Sci., 15: 1409-1425.
67. Chirila, T.V. and Zainuddin. (2007). Calcification of Synthetic Polymers Functionalized with Negatively Ionizable Groups: A Critical Review, React. Funct. Polym., 67: 165-172.
68. Chirila, T.V., Zainuddin, Hill, D.J.T., Whittaker, A.K. and Kemp, A. (2007). Effect of Phosphate Functional Groups on the Calcification Capacity of Acrylic Hydrogels, Acta Biomater., 3: 95-102.
69. Qiu, Q., Sayer, M., Kawaja, M., Shen, X. and Davies, J.E. (1998). Attachment, Morphology, and Protein Expression of Rat Marrow Stromal Cells Cultured on Charged Substrate Surfaces, J. Biomed. Mater. Res., 42: 117-127.
70. Massia, S.P. and Hubbell, J.A. (1992). Immobilized Amines and Basic Amino Acids as Mimetic Heparin-binding Domains for Cell Surface Proteoglycanmediated Adhesion, J. Biol. Chem., 267: 10133-10141.
71. Kishida, A., Iwata, H., Tamada, Y. and Ikada, Y. (1991). Cell Behaviour on Polymer Surfaces Grafted with Non-ionic and Ionic Monomers, Biomaterials, 12: 786-792.
72. Webb, K., Hlady, V. and Tresco, P.A. (1998). Relative Importance of Surface Wettability and Charged Functional Groups on NIH 3T3 Fibroblast Attachment, Spreading, and Cytoskeletal Organization, J. Biomed. Mater. Res., 41: 422-430.
73. Kleinfeld, D., Kahler, K.H. and Hockberger, P.E. (1988). Controlled Outgrowth of Dissociated Neurons on Patterned Substrates, J. Neurosci., 8: 4098-4120.
74. Curtis, A.S.G. and Forrester, J.V. (1984). The Competitive Effects of Serum Proteins on Cell Adhesion, J. Cell Sci., 71: 17-35.
75. Shelton, R.M., Rasmussen, A.C. and Davies, J.E. (1988). Protein Adsorption at the Interface between Charged Polymer Substrata and Migrating Osteoblasts, Biomaterials, 9: 24-29.
76. Curtis, A.S.G., Forrester, J.V., McInnes, C. and Lawrie, F. (1983). Adhesion of Cells to Polystyrene Surfaces, J. Cell. Biol., 97: 1500-1506.
77. Skladchikova, G., Ronn, L.C.B., Berezin, V. and Bock, E. (1999). Extracellular Adenosine Triphosphate Affects Neural Cell Adhesion Molecule (NCAM)-mediated Cell Adhesion and Neurite Outgrowth, J. Neurosci. Res., 57: 207-218.
78. Kishida, A., Taguchi, T., Yanagi, M., Aikou, T. and Akashi, M. (2000). Study of Polymer-apatite Composites as Model Systems for Calcification and Novel Biomaterials, Kobunshi Ronbunshu, 57: 159-166.
79. Mehta, J.S., Futter, C.E., Sandeman, S.R., Faragher, R.G.A., Hing, K.A., Tanner, K.E. and Allan, B.D.S. (2005). Hydroxyapatite Promotes Superior Keratocyte Adhesion and Proliferation in Comparison with Current Keratoprosthesis Skirt Materials, Br. J. Ophthalmol., 89: 1356-1362.
80. Teng, M.H. and Rifkin, D.B. (1979). Fibronectin from Chicken Embryo Fibroblasts Contains Covalently Bound Phosphate, J. Cell. Biol., 80: 784-791.
81. Lydon, M.J. and Clay, C.S. (1985). Substratum Topography and Cell Traction on Sulphuric Acid Treated Bacteriological-grade Plastic, Cell Biol. Int. Rep., 9: 911-921.
82. Wójciak-Stothard, B., Curtis, A., Monaghan, W., Macdonald, K. and Wilkinson, C. (1996). Guidance and Activation of Murine Macrophages by Nanometric Scale Topography, Exp. Cell. Res., 223: 426-435.
83. Curtis, A.S.G. and Wilkinson, C.D.W. (1998). Reactions of Cells to Topography, J. Biomater. Sci. Polym. Edn., 9: 1313-1329.
84. Folch, A. and Toner, M. (2000). Microengineering of Cellular Interactions, Annu. Rev. Biomed. Eng., 2: 227-256.
85. Jung, D.R., Kapur, R., Adams, T., Giuliano, K.A., Marksich, M., Craighead, H.G. and Taylor, D.L. (2001). Topographical and Physicochemical Modification of Material Surface to Enable Patterning of Living Cells, Crit. Rev. Biotechnol., 21: 111-154.
86. Vogt-Eisele, A.K., Offenhäusser, A. and Knoll, W. (2005). Cell Guidance through Surface Cues, Surfaces and Interfaces for Biomaterials, Vadgama, P. (ed.), Woodhead Publishing Ltd., Cambridge, UK.