The use of isolated human lymphocytes in mycotoxin cytotoxicity testing

International Journal of Molecular Sciences

Volumn 9, Issue 8, 2008, Pages 1515-1526

  Maenetje, Pholo W ^a   De Villiers, Neil ^a   Dutton, Mike F ^a  

^a UNIVERSITY OF JOHANNESBURG   (South Africa)

Author keywords

Cytotoxicity; Lymphocytes; Mycotoxicosis; Mycotoxin screening; Mycotoxins

Indexed keywords

FORMAZAN; FUMONISIN B1; METHYLTETRAZOLIUM BROMIDE; MYCOTOXIN; OCHRATOXIN; TETRAZOLIUM; UNCLASSIFIED DRUG; VOMITOXIN;

ARTICLE; BARLEY; CELL ISOLATION; CELL VIABILITY; CONTROLLED STUDY; CORRELATION ANALYSIS; CYTOTOXICITY TEST; FALSE POSITIVE RESULT; HUMAN; HUMAN CELL; LYMPHOCYTE; MALT; QUANTITATIVE ANALYSIS; SPECTROPHOTOMETRY;

HORDEUM;

EID: 50849121453     PISSN: None     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms9081515     Document Type: Article

References (23)

1. Yu, J.H.; Keller, N. Regulation of secondary metabolism in filamentous fungi. Annu. Rev. Phytopathol. 2005, 43, 437-458.
2. Cole, R.J.; Cox, R.H. Handbook of Toxic Fungal Metabolites. Academic Press: New York, 1981.
3. Frisvad, J.C.; Thrane, U.; Samson, R.A.; Pitt, J.I. Important mycotoxins and the fungi which produce them. Adv. Exp. Med. Biol. 2006, 571, 3-31.
4. Prelusky, D.B.; Rotter, B.A.; Rotter, R.G. Toxicology of mycotoxins. In: Mycotoxins in Grain. Compounds Other Than Aflatoxins; Miller, J.D., Trenholm, H.L., Eds.; Eagan Press: St. Paul, MN, USA, 1994; pp. 359-404.
5. Veldman, V.; Meijst, J.A.C.; Borgreve, J.; Heers-Van der Tol, J.J. Carry over of aflatoxin from cow's food to milk. Anim. Prod. 1992, 55, 163-168.
6. Gelderblom, W.C.A.; Jaskiewicz, K.; Marasas, W.F.O.; Thiel, P.G.; Horak, M.; Vleggaar, R.; Kriek, N.P.J. 1988 Fumonisins- novel mycotoxins with cancer promoting activity produced by Fusarium moniliforme. Appl. Environ. Microbiol. 1988, 4, 1806-1811.
7. Robb, J.; Norval, M. Comparison of cytotoxicity and thin-layer chromatography methods for detection of mycotoxins. Appl. Environ. Microbiol. 1983, 46, 948-50.
8. Cetin, Y.; Bullerman, L.B. Cytotoxicity of Fusarium mycotoxins to mammalian cell cultures as determined by the MTT assay. Food Chem. Toxicol. 2005, 43, 755-764.
9. Smith, J.E.; Anderson, J.G.; Lewis, C.W.; Murad, Y.M. Cytotoxic fungal spores in the indoor atmosphere of the damp domestic environment. FEMS Microbiol. Lett. 1992, 100, 337-343.
10. Widestrand, J.; Lundh, T.; Pettersson, H.; Lindberg, J.E. A rapid and sensitive cytotoxicity screening assay for trichothecenes in cereal samples. Food Chem. Toxicol. 2003, 41, 1307-1313
11. Hanelt, M.; Garies, M.; Kollarczik, B. Cytotoxicity of mycotoxins evaluated by the MTT-cell culture assay. Mycopathology 1994, 128, 167-174.
12. Patterson, D.S.P.; Roberts, B.A. Mycotoxins in animal feedstuffs: Sensitive thin layer chromatographic detection of aflatoxin, ochratoxin A, sterigmatocystin, zearalenone and T2 toxin. J. Assoc. Off.. Anal. Chem. 1979, 62, 1265-1267.
13. Boyum, A. Isolation of mononuclear cells and granulocytes from human blood. J. Soc. Clin. Labs. 1968, 21, 77-78.
14. 3 in foods and feeds. J. Assoc. Off. Anal. Chem. Internat. 1992, 75, 313-318.
15. Eke, Z.; Torkos, K. N, N-dimethyl-trimethylsilyl-carbamate as derivatizing agent in gas chromatography. Microchem. J. 2003, 44, 43-46.
16. 1, diethylnitrosamine and catechol on the SNO oesophageal cancer cell line. Environ. Health Perspec. 2002, 110, 813-815.
17. Wang, E.; Norred, W.P.; Bacon, C.W.; Riley, R.T.; Merrill, A.H. Inhibition of sphingolipid biosynthesis by fumonisins: Implications for diseases associated with Fusarium moniliforme. J. Biol. Chem. 1991, 266, 14486-14490.
18. Riley, R.T.; Wang, E.; Schroeder, J.J.; Smith, E.R.; Plattner, R.D.; Abbas, H.; Yoo, H.S.; Merrill, A.H. Evidence for disruption of sphingolipid metabolism as a contributing factor in the toxicity and carcinogenicity of fumonisins. Nat. Tox. 1996, 4, 3-15.
19. Rheeder, I.P.; Marasas, W.F.O.; Thiel, P.G.; Sydenham, E.W.; Shephard, G.S.; Van Schalkwyk, D.J. Fusarium moniliforme and fumonisins in corn in relation to human esophageal cancer in Transkei. Pathophysiology 1992, 82, 353-357.
20. Pestka, J.J.; Bondy, G.S. Alteration of immune function following dietary mycotoxin exposure. Can. J. Physiol. Pharmacol. 1990, 66, 1009-1016.
21. Rotter, B.A.; Prelusky, D.B. Pestka, J.J. Toxicology of deoxynivalenol (vomitoxin). J. Toxicol. Environ. Health 1996, 48, 1-34.
22. Visconti, A.; Minervini, F.; Lucivero, G.; Gambatesa, V. Cytotoxic and immune-effects of Fusarium mycotoxins using rapid colorimetric bioassay. Mycopathol. 1991, 113, 181-186.
23. Bungar, J.; Westphal, G.; Monnich, A.; Hhinnendahl, B.; Hallier, E.; Muller, M. 2004 Cytotoxicity of occupationally and environmentally relevant mycotoxins. Toxicol. 2004, 202, 199-211.