Imaging dynamic cell-cell junctional coupling in vivo using Trojan-LAMP

Nature Methods

Volumn 5, Issue 9, 2008, Pages 835-841

  Guo, Yan Ming ^a   Chen, Shiuhwei ^a   Shetty, Premnath ^b   Zheng, Genhua ^a   Lin, Rueyling ^b   Li, Wen Hong ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEXTRAN CANPE HCC; DYE; GAP JUNCTION PROTEIN; PROTEIN P2; PROTEIN P3; PROTEIN P4; PROTEIN PRECURSOR;

ARTICLE; CAENORHABDITIS ELEGANS; CELL COMMUNICATION; CELL COMPARTMENTALIZATION; CELL JUNCTION; CONTROLLED STUDY; EMBRYO DEVELOPMENT; FEMALE; FLUORESCENCE ANALYSIS; GAP JUNCTION; GERM CELL; IMAGE ANALYSIS; IN VIVO STUDY; NON INVASIVE MEASUREMENT; NONHUMAN; OOCYTE; PHOTOACTIVATION; PHOTOLYSIS; PRIORITY JOURNAL; SIGNAL DETECTION; SOMATIC CELL; TROJAN LOCAL ACTIVATION OF MOLECULAR FLUORESCENT PROBES;

ANIMALS; BLASTOMERES; CAENORHABDITIS ELEGANS; CELL COMMUNICATION; FLUORESCENT DYES; GAP JUNCTIONS; MICROSCOPY, FLUORESCENCE; PHOTOLYSIS;

CAENORHABDITIS ELEGANS;

EID: 50849110484     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth.1238     Document Type: Article

References (30)

1. Goodenough, D.A., Goliger, J.A. & Paul, D.L. Connexins, connexons, and intercellular communication. Annu. Rev. Biochem. 65, 475-502 (1996).
2. Bennett, M.V. & Zukin, R.S. Electrical coupling and neuronal synchronization in the Mammalian brain. Neuron 41, 495-511 (2004).
3. Harris, A.L. Emerging issues of connexin channels: Biophysics fills the gap. Q. Rev. Biophys. 34, 325-472 (2001).
4. Nicholson, B.J. Gap junctions - from cell to molecule. J. Cell Sci. 116, 4479-4481 (2003).
5. 2+ influx inhibits cell coupling. Nat. Methods 2, 55-62 (2005).
6. Dakin, K. & Li, W.H. Infrared-LAMP: Two-photon uncaging and imaging of gap junctional communication in three dimensions. Nat. Methods 3, 959 (2006).
7. Zhao, Y. et al. New caged coumarin fluorophores with extraordinary uncaging cross sections suitable for biological imaging applications. J. Am. Chem. Soc. 126, 4653-4663 (2004).
8. White, J.G., Southgate, E., Thomson, J.N. & Brenner, S. The structure of the nervous system of the nematode Caenorhabditis elegans. Phil. Trans. R. Soc. Lond. B 314, 1-340 (1986).
9. Phelan, P. & Starich, T.A. Innexins get into the gap. Bioessays 23, 388-396 (2001).
10. Herve, J.C., Phelan, P., Bruzzone, R. & White, T.W. Connexins, innexins and pannexins: Bridging the communication gap. Biochim. Biophys. Acta 1719, 3-5 (2005).
11. Hua, V.B. et al. Sequence and phylogenetic analyses of 4 TMS junctional proteins of animals: Connexins, innexins, claudins and occludins. J. Membr. Biol. 194, 59-76 (2003).
12. Mitchison, T.J., Sawin, K.E., Theriot, J.A., Gee, K. & Mallavarapu, A. Caged fluorescent probes. Methods Enzymol. 291, 63-78 (1998).
13. Squirrell, J.M., Wokosin, D.L., White, J.G. & Bavister, B.D. Long-term two-photon fluorescence imaging of mammalian embryos without compromising viability. Nat. Biotechnol. 17, 763-767 (1999).
14. Sulston, J.E., Schierenberg, E., White, J.G. & Thomson, J.N. The embryonic cell lineage of the nematode Caenorhabditis elegans. Dev. Biol. 100, 64-119 (1983).
15. Goodenough, D.A. & Paul, D.L. Beyond the gap: Functions of unpaired connexon channels. Nat. Rev. Mol. Cell Biol. 4, 285-294 (2003).
16. Bao, Z. et al. Automated cell lineage tracing in. Caenorhabditis elegans. Proc. Natl. Acad. Sci. USA (2006).
17. Strehlow, D. & Gilbert, W. A fate map for the 1st cleavages of the zebrafish. Nature 361, 451-453 (1993).
18. Wolke, U., Jezuit, E.A. & Priess, J.R. Actin-dependent cytoplasmic streaming in C. elegans oogenesis. Development 134, 2227-2236 (2007).
19. Rozental, R., Srinivas, M. & Spray, D.C. How to close a gap junction channel in Connexins Methods and Protocols (eds. Bruzzone, R. & Giaume, C.) 447-476 (Humana Press, Totowa, New Jersey, 2001).
20. Simpson, I., Rose, B. & Loewenstein, W.R. Size limit of molecules permeating the junctional membrane channels. Science 195, 294-296 (1977).
21. Altun, Z.F. & Hall, D.H. Handbook of C. elegans anatomy. http://www.wormatlas.org/handbook/contents.htm. (2005).
22. Levin, M. Isolation and community: A review of the role of gap-junctional communication in embryonic patterning. J. Membr. Biol. 185, 177-192 (2002).
23. Bossinger, O. & Schierenberg, E. Cell-cell communication in the embryo of Caenorhabditis elegans. Dev. Biol. 151, 401-409 (1992).
24. Bossinger, O. & Schierenberg, E. The use of fluorescent marker dyes for studying intercellular communication in nematode embryos. Int. J. Dev. Biol. 40, 431-439 (1996).
25. Lopresti, V., Macagno, E.R. & Levinthal, C. Structure and development of neuronal connections in isogenic organisms: Transient gap junctions between growing optic axons and lamina neuroblasts. Proc. Natl. Acad. Sci. USA 71, 1098-1102 (1974).
26. Wolszon, L.R., Gao, W.Q., Passani, M.B. & Macagno, E.R. Growth cone "collapse" in vivo: Are inhibitory interactions mediated by gap junctions? J. Neurosci. 14, 999-1010 (1994).
27. Montoro, R.J. & Yuste, R. Gap junctions in developing neocortex: A review. Brain Res. Brain Res. Rev. 47, 216-226 (2004).
28. Starich, T.A., Miller, A., Nguyen, R.L., Hall, D.H. & Shaw, J.E. The Caenorhabditis elegans innexin INX-3 is localized to gap junctions and is essential for embryonic development. Dev. Biol. 256, 403-417 (2003).
29. Chuang, C.F., Vanhoven, M.K., Fetter, R.D., Verselis, V.K. & Bargmann, C.I. An innexin-dependent cell network establishes left-right neuronal asymmetry in C. elegans. Cell 129, 787-799 (2007).
30. 2+ imaging of local neuronal circuits. Neuron 53, 789-803 (2007).