LAMP, a new imaging assay of gap junctional communication unveils that Ca2+ influx inhibits cell coupling

Nature Methods

Volumn 2, Issue 1, 2005, Pages 55-62

  Dakin, Kenneth ^a   Zhao, YuRui ^a   Li, Wen Hong ^a  

^a UNIVERSITY OF TEXAS AT DALLAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM ION; FLUORESCENT DYE; GAP JUNCTION PROTEIN; IONOMYCIN; TRACER; CALCIUM;

ARTICLE; CALCIUM CELL LEVEL; CALCIUM TRANSPORT; CELL COMMUNICATION; CELL LABELING; CELL MEMBRANE; CELL VOLUME; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; GAP JUNCTION; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; MOLECULAR IMAGING; PATCH CLAMP; PHOTOACTIVATION; PRIORITY JOURNAL; ANIMAL; CELL CULTURE; CELL JUNCTION; CHEMICAL MODEL; FIBROBLAST; METABOLISM; METHODOLOGY; PHYSIOLOGY; TIME;

ANIMALS; CALCIUM; CELL COMMUNICATION; CELL MEMBRANE; CELLS, CULTURED; FIBROBLASTS; FLUORESCENT DYES; GAP JUNCTIONS; HUMANS; INTERCELLULAR JUNCTIONS; MICROSCOPY, FLUORESCENCE; MODELS, CHEMICAL; TIME FACTORS;

EID: 18744395238     PISSN: 15487091     EISSN: None     Source Type: Journal    
DOI: 10.1038/nmeth730     Document Type: Article

References (32)

1. De Maio, A., Vega, V.L. & Contreras, J.E. Gap junctions, homeostasis, and injury. J. Cell. Physiol. 191, 269-282 (2002).
2. Bennett, M.V. & Zukin, R.S. Electrical coupling and neuronal synchronization in the Mammalian brain. Neuron 41, 495-511 (2004).
3. Peracchia, C. (ed.) Gap junctions molecular basis of cell communication in health and disease (Academic Press, 2000).
4. Goodenough, D.A., Goliger, J.A. & Paul, D.L. Connexins, connexons and intercellular communication. Annu. Rev. Biochem. 65, 475-502 (1996).
5. Harris, A.L. Emerging issues of connexin channels: biophysics fills the gap. Q. Rev. Biophys. 34, 325-472 (2001).
6. Wade, M.H., Trosko, J.E. & Schindler, M. A fluorescence photobleaching assay of gap junction-mediated communication between human cells. Science 232, 525-528 (1986).
7. Van Rijen, H.V.M., Wilders, R., Rook, M.B. & Jongsma, H.J. in Connexin methods and protocols (eds. Bruzzone, R. & Giaume, C.) 269-292 (Humana, Totowa, New Jersey, 2001).
8. Valiunas, V., Beyer, E.C. & Brink, P.R. Cardiac gap junction channels show quantitative differences in selectivity. Circ. Res. 91, 104-111 (2002).
9. Wilders, R. & Jongsma, H.J. Limitations of the dual voltage clamp method in assaying conductance and kinetics of gap junction channels. Biophys. J. 63, 942-953 (1992).
10. Zhao, Y. et al. New caged coumarin fluorophores with extraordinary uncaging cross sections suitable for biological imaging applications. J. Am. Chem. Soc. 126, 4653-4663 (2004).
11. Davidson, J.S. & Baumgarten, I.M. Glycyrrhetinic acid derivatives: a novel class of inhibitors of gap-junctional intercellular communication. Structure-activity relationships. J. Pharmacol. Exp. Ther. 246, 1104-1107 (1988).
12. Peters, R. Nuclear envelope permeability measured by fluorescence microphotolysis of single liver cell nuclei. J. Biol. Chem. 258, 11427-11429 (1983).
13. Biegon, R.P., Atkinson, M.M., Liu, T.F., Kam, E.Y. & Sheridan, J.D. Permeance of Novikoff hepatoma gap junctions: quantitative video analysis of dye transfer. J. Membr. Biol. 96, 225-233 (1987).
14. Oliveira-Castro, G.M. & Loewenstein, W.R. Junctional membrane permeability: effects of divalent cations. J. Membr. Biol. 5, 51-77 (1971).
15. Noma, A. & Tsuboi, N. Dependence of junctional conductance on proton, calcium and magnesium ions in cardiac paired cells ofguinea-pig. J. Physiol. (Lond.) 382, 193-211 (1987).
16. Lazrak, A. & Peracchia, C. Gap junction gating sensitivity to physiotogical internal calcium regardless of pH in Novikoff hepatoma cells. Biophys. J. 65, 2002-2012 (1993).
17. Spray, D.C., Stern, J.H., Harris, A.L. & Bennett, M.V. Gap junctional conductance: comparison of sensitivities to H and Ca ions. Proc. Natl. Acad. Sci. USA 79, 441-445 (1982).
18. Firek, L. & Weingart, R. Modification of gap junction conductance by divalent cations and protons in neonatal rat heart cells. J. Mol. Cell. Cardiol. 27, 1633-1643 (1995).
19. Berridge, M.J. Inositol trisphosphate and calcium signalling. Nature 361, 315-325 (1993).
20. Takemura, H., Hughes, A.R., Thastrup, O. & Putney, J.W., Jr. Activation of calcium entry by the tumor promoter thapsigargin in parotid acinar cells. Evidence that an intracellular calcium pool and not an inositol phosphate regulates calcium fluxes at the plasma membrane. J. Biol. Chem. 264, 12266-12271 (1989).
21. 2+ indicators with greatly improved fluorescence properties. J. Biol. Chem. 260, 3440-3450 (1985).
22. Minta, A., Kao, J.P.Y. & Tsien, R.Y. Fluorescent Indicators For Cytosolic Calcium Based On Rhodamine and Fluorescein Chromophores. J. Biol. Chem. 264, 8171-8178 (1989).
23. 2+ transport in lacrimal acinar cells. Am. J. Physiol. 258, C1006-C1015 (1990).
24. 2+ beneath the plasma membrane of living A7r5 cells. EMBO J. 16, 1575-1581 (1997).
25. 2+ beneath the plasma membrane in stimulated neutrophils. Biochem. Biophys. Res. Commun. 248, 679-683 (1998).
26. Unwin, P.N. & Ennis, P.D. Two configurations of a channel-forming membrane protein. Nature 307, 609-613 (1984).
27. Muller, D.J., Hand, G.M., Engel, A. & Sosinsky, G.E. Conformational changes in surface structures of isolated connexin 26 gap junctions. EMBO J. 21, 3598-3607 (2002).
28. Turin, L. & Warner, A.E. Intracellular pH in early Xenopus embryos: its effect on current flow between blastomeres. J. Physiol. (Lond.) 300, 489-504 (1980).
29. Peracchia, C., Sotkis, A., Wang, X.G., Peracchia, L.L. & Persechini, A. Calmodulin directly gates gap junction channels. J. Biol. Chem. 275, 26220-26224 (2000).
30. Jurado, L.A., Chockalingam, P.S. & Jarrett, H.W. Apocalmodulin. Physiol. Rev. 79, 661-682 (1999).
31. DeVries, S.H. & Schwartz, E.A. Hemi-gap-junction channels in solitary horizontal cells of the catfish retina. J. Physiol. (Lond.) 445, 201-230 (1992).
32. Goodenough, D.A. & Paul, D.L. Beyond the gap: functions of unpaired connexon channels. Nat. Rev. Mol. Cell Biol. 4, 285-294 (2003).