Reducing cloning artifacts for recovery of allelic sequences by T7 endonuclease I cleavage and single re-extension of PCR products - A benchmark

Gene

Volumn 423, Issue 1, 2008, Pages 92-95

  Saitoh, Kenji ^a   Chen, Wei Jen ^b  

^a FISHERIES RESEARCH AGENCY   (Japan)

^b SAINT LOUIS UNIVERSITY   (United States)

Author keywords

Chimera; Heteroduplex; Mismatch repair; Nuclear gene; Recombinant; TA cloning; Template switching

Indexed keywords

ENDONUCLEASE;

ALLELE; ARTICLE; ARTIFACT; FISH; GENE SEQUENCE; HAPLOTYPE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ALLELES; ANIMALS; BASE SEQUENCE; CHIMERA; CLONING, MOLECULAR; CYPRINIFORMES; DEOXYRIBONUCLEASE I; DNA PRIMERS; HAPLOTYPES; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION;

EID: 50549085993     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.gene.2008.06.025     Document Type: Article

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