Mutations on N-terminal region of Taiwan cobra phospholipase A2 result in structurally distorted effects

Journal of Peptide Science

Volumn 14, Issue 8, 2008, Pages 890-897

  Chiou, Yi Ling ^a   Lin, Shinne Ren ^b   Chang, Long Sen ^a  

^a NATIONAL SUN YAT SEN UNIVERSITY   (Taiwan)

^b KAOHSIUNG MEDICAL UNIVERSITY   (Taiwan)

Author keywords

Catalytic site; Energy transfer; Interfacial binding site; JV terminal mutation; Phospholipase A2; Trp fluorescence

Indexed keywords

8 ANILINO 1 NAPHTHALENESULFONIC ACID; ASPARAGINE; BARIUM ION; CALCIUM ION; HEPTAPEPTIDE; LYSINE; METHIONINE; PHOSPHOLIPASE A2; TRYPTOPHAN;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING KINETICS; CONTROLLED STUDY; ENERGY TRANSFER; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME PURIFICATION; ENZYME STRUCTURE; ESCHERICHIA COLI; FLUORESCENCE ANALYSIS; GENE DELETION; GENE EXPRESSION; GENE MUTATION; HYDROPHOBICITY; MICROENVIRONMENT; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN MODIFICATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SNAKE; TAIWAN;

ANIMALS; BARIUM; BINDING SITES; CALCIUM; COBRA; COBRA VENOMS; ENERGY TRANSFER; ENZYME ACTIVATION; FLUORESCENT DYES; GENE EXPRESSION PROFILING; GENETIC VARIATION; MUTATION; PHOSPHOLIPASES A2; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SPECTROMETRY, FLUORESCENCE; STRUCTURE-ACTIVITY RELATIONSHIP; TAIWAN;

ESCHERICHIA COLI; NAJA ATRA;

EID: 50449090579     PISSN: 10752617     EISSN: 10991387     Source Type: Journal    
DOI: 10.1002/psc.1020     Document Type: Article

References (30)

1. 2 Enzymes: Structure, Function and Mechanism, Kini RM (ed.). John Wiley and Son: Chichester, 1997; 1-28.
2. 2 from studies on native and chimeric proteins. J. Biol. Chem. 2005; 280: 36773-36783.
3. 2 determines productive-mode orientation of the enzyme at the membrane surface. J. Mol. Biol. 2004; 344: 71-89.
4. 2 suggest allosteric coupling between membrane binding and catalytic sites. Biophys. J. 2003; 84: 1773-1783.
5. 2. Science 1990; 250: 1541-1546.
6. 2 in a complex with a transition-state analogue. Science 1990; 250: 1560-1563.
7. 2. Protein J. 2006; 25: 127-134.
8. 2 covalently inhibited by p-bromo-phenacyl-bromide. J. Mol. Biol. 1988; 200: 181-188.
9. 2 modified by p-bromo-phenacyl-bromide. Toxicon 1998; 36: 875-886.
10. 43Ca NMR. FEBS Lett. 1981; 123: 115-117.
11. 2. J. Protein Chem. 1996; 15: 701-707.
12. 2. J. Biochem. (Tokyo) 1998; 124: 764-768.
13. Thannhauser TW, Scheraga HA. Reversible blocking of half-cystine residues of proteins and an irreversible specific deamidation of asparagine-67 of S-sulforibonuclease under mild conditions. Biochemistry 1985; 24: 7681-7688.
14. Chang LS, Wu PF, Lin J. cDNA sequence analysis and expression of cardiotoxins from Taiwan Cobra. Biochem. Biophys. Res. Commun. 1996; 219: 116-121.
15. 2 in Escherichia coli, a fully active enzyme generated by hydrolyzing with aminopeptidase. Biochem. Biophys. Res. Commun. 1996; 225: 990-996.
16. Eftink MR, Ghiron CA. Fluorescence quenching studies with proteins. Anal. Biochem. 1981; 114: 199-227.
17. Chang LS, Wen EY. Hung JJ, Chang CC. Energy transfer from tryptophan residues of proteins to 8-anilinonaphthalene-1-sulfonate. J. Protein Chem. 1994; 13: 635-640.
18. 2 from Naja naja atra (Taiwan cobra) snake venom. Biochim. Biophys. Acta 1993; 1202: 216-220.
19. 2. Biochemistry 1999; 38: 16290-16297.
20. Cardamone M, Puri NK. Spectrofluorimetric assessment of the surface hydrophobicity of proteins. Biochem. J. 1992; 282: 589-593.
21. Jones BE, Jennings PA, Pierre RA, Mattews CR. Development of nonpolar surfaces in the folding of Escherichia coli dihydrofolate reductase detected by 1-anilinonaphthalene-8-sulfonate binding. Biochemistry 1994; 33: 15250-15258.
22. Stryer L. Fluorescence spectroscopy of proteins. Science 1968: 162: 526-533.
23. 2 from Naja naja atra (Taiwan cobra) and Naja nigricollis (spitting cobra) venoms. Toxicon 1988: 26: 721-731.
24. 2 from the venom of Naja naja atra (Taiwan cobra). Toxicon 1981; 19: 645-659.
25. Campbell ID, Dwek RA. Biological Spectroscopy. The Benjamin/ Cummings Publishing Company Inc: Menlo park, CA, 1984; 91-126.
26. Lakowicz JR. Principles of Fluorescence Spectroscopy. Kluwer Academic/Plenum Publishers: New York, 1999.
27. 2 by trinitrophenylation of lysine residues. J. Protein Chem. 1997; 16: 51-57.
28. 2 and N-terminal variant: kinetic parameters and response to inhibitors. J. Biochem. (Tokyo) 1993; 113: 734-737.
29. 2 engineering. Probing the structural and functional roles of N-terminal residues with site-directed mutagenesis, X-ray, and NMR. Biochemistry 1995; 34: 7322-7334.
30. 2 and functional implications. Biochemistry 2006; 45: 12436-12447.