Development and application of a PCR diagnostic assay for the accurate and rapid identification of the nematophagous fungus Duddingtonia flagrans

Mycological Research

Volumn 112, Issue 9, 2008, Pages 1026-1030

  Kelly, Paula ^a,b   Good, Barbara ^a   Fitzpatrick, Richard ^a   Hanrahan, J P ^a   de Waal, Theo D T ^b  

^a TEAGASC   (Ireland)

^b UNIVERSITY COLLEGE DUBLIN   (Ireland)

Author keywords

Biocontrol; Nematodes; Nematogenous fungi; Soil fungi; Species specific primers

Indexed keywords

FUNGAL DNA; PRIMER DNA; RIBOSOMAL SPACER DNA;

ASSAY; BIOASSAY; DETECTION METHOD; FUNGUS; IDENTIFICATION METHOD; LEAF LITTER; NEMATODE; POLYMERASE CHAIN REACTION; POTENTIAL BIOCONTROL AGENT; RUMINANT;

ANIMAL; ARTICLE; ASCOMYCETES; CLASSIFICATION; EVALUATION; FECES; FUNGUS SPORE; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; NEMATODE; PHYSIOLOGY; PLANT LEAF; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENCE; TIME;

ANIMALS; ASCOMYCOTA; DNA PRIMERS; DNA, FUNGAL; DNA, RIBOSOMAL SPACER; FECES; MYCOLOGICAL TYPING TECHNIQUES; NEMATODA; PLANT LEAVES; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOIL MICROBIOLOGY; SPECIES SPECIFICITY; SPORES, FUNGAL; TIME FACTORS;

EURASIA; EUROPE; IRELAND; WESTERN EUROPE;

BOVIDAE; DUDDINGTONIA; DUDDINGTONIA FLAGRANS; FUNGI; NEMATODA;

EID: 50349103473     PISSN: 09537562     EISSN: 14698102     Source Type: Journal    
DOI: 10.1016/j.mycres.2008.02.005     Document Type: Article

References (28)

1. Abd-Elsalam K.A., Aly I.N., Abdel-Satar M.A., Khalil M.S., and Verreet J.A. PCR identification of Fusarium genus based on nuclear ribosomal-DNA sequence data. African Journal of Biotechnology 2 (2003) 82-85
2. Atkins S., and Clark I.M. Fungal molecular diagnostics: a mini review. Journal of Applied Genetics 45 (2004) 3-15
3. Atkins S., Hidalgo-Diaz L., Clark I.M., Norton C.O., Montes de Oca N., Gray P.A., and Kerry B. Approaches for monitoring the release of Pochonia chlamydosporia var. catenulata, a biocontrol agent of root-knot nematodes. Mycological Research 107 (2003) 206-212
4. Atkins S.D., Clark I.M., Pande S., Hirsch P.R., and Kerry B.R. The use of real-time PCR and species-specific primers for the identification and monitoring of Paecilomyces lilacinus. FEMS Microbiology Ecology 51 (2005) 257-264
5. Atkins S.D., Clark I.M., Sosnowska D., Hirsch P.R., and Kerry B.R. Detection and quantification of Plectophaerella cucumerina, a potential biological control agent of potato cyst nematodes, by using conventional PCR, real-time PCR, selective media and baiting. Applied and Environmental Microbiology 69 (2003) 4788-4793
6. Avis T.J., Hamelin R.C., and Belamger R.R. Approaches to molecular characterization of fungal biocontrol agents: some case studies. Canadian Journal of Plant Pathology 23 (2001) 8-12
7. Chandrawathani P., Jamnah O., Adran M., Waller P.J., Larsen M., and Gillespie A.T. Field study on the biological control of nematode parasites of sheep in the tropics, using microfungus Duddingtonia flagrans. Veterinary Parasitology 120 (2004) 177-187
8. Chandrawathani P., Jamnah O., Waller P.J., Hoglund J., Larsen M., and Zahari W.M. Nematophagous fungi as a biological control agent for nematode parasites of small ruminants in Malaysia: a special emphasis on Duddingtonia flagrans. Veterinary Parasitology 33 (2002) 685-696
9. Chartier C., and Pors I. Effects of nematophagous fungus, Duddingtonia flagrans, on larval development of goat parasitic nematodes: plot study. Veterinary Research 34 (2003) 221-230
10. Cooke R.C. Two nematode-trapping hyphomycetes, Duddingtonia flagrans gen. et comb. nov. and Monacrosporium mutabilis sp. nov. Transactions of the British Mycological Society 53 (1969) 315-318
11. Duddington C.L. A new predacious species of Trichothecium. Transactions of the British Mycological Society 32 (1950) 284-287
12. Durand D.T., Boshoff H.M., Micheal L.M., and Krecek R.C. Survey of nematophagous fungi in South Africa. Onderstepoort Journal of Veterinary Research 72 (2005) 185-187
13. Faedo M., Barnes E.H., Dobson R.J., and Waller P.J. The potential of nematophagous to control the free-living stages of nematode parasites of sheep: pasture plot study with Duddingtonia flagrans. Veterinary Parasitology 76 (1998) 129-135
14. Fontenot M.E., Miller J.E., Pena M.T., Larsen M., and Gillespie A. Efficiency of feeding Duddingtonia flagrans chlamydospores to grazing ewes on reducing availability of parasitic nematodes larvae on pasture. Veterinary Parasitology 118 (2003) 203-213
15. Ghahfarokhi S.M., Abyaneh M.R., Bahadori S.R., Eslami A., Zare A., and Ebrahimi M. Screening of soil and sheep samples for predacious fungi: isolation and characterisation of the nematode-trapping fungus Arthrobotrys oligospora. Iranian Biomedical Journal 8 (2004) 135-142
16. Gray N.F. Ecology of nematophagous fungi: distribution and habitat. Association of Applied Biologists 102 (1983) 501-509
17. Hirsch P.R., Mauchline T.M., Mendum T.A., and Kerry B. Detection of nematophagous fungus Verticillium chlamydosporium in nematode-infested plant roots using PCR. Mycological Research 104 (2000) 435-439
18. Larsen M. Biological control of helminths. International Journal of Parasitology 29 (1999) 139-146
19. Larsen M., Faedo M., and Waller P.J. The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: survey for the presence of fungi in fresh faeces of grazing livestock in Australia. Veterinary Parasitology 53 (1994) 275-281
20. Larsen M., Faedo M., Waller P.J., and Hennessy D.R. The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: studies with Duddingtonia flagrans. Veterinary Parasitology 76 (1998) 121-128
21. Larsen M., Wolstrup J., Henriksen S.A., Gronvold J., and Nansen P. In vivo passage through calves of nematophagous fungi selected for biocontrol of parasitic nematodes. Journal of Helminthology 66 (1992) 137-141
22. Mendoza de Gives P., Crespo J.F., Rodriguez D.H., Prats V.V., Hernandez E.L., and Fernandez G.E.O. Biological control of Haemonchus contortus infective larvae in ovine faeces by administering an oral suspension of Duddingtonia flagrans chlamydospores to sheep. Journal of Helminthology 72 (1998) 343-347
23. SAS Institute, Inc. SAS/STAT User's Guide, Version 8 (2000), SAS Institute, Inc., Cary, North Carolina, USA
24. Skipp R.A., Yeates G.W., Chen L.Y., and Glare T.R. Occurrence, morphological characteristics and ribotyping of New Zealand isolates of Duddingtonia flagrans, a candidate for biocontrol of animal parasitic nematodes. New Zealand Journal of Agricultural Research 45 (2002) 187-196
25. Terrill T.H., Larsen M., Samples O., Husted S., Miller J.E., Kaplan R.M., and Gelaye S. Capability of nematode-trapping fungus Duddingtonia flagrans to reduce infective larvae of gastrointestinal nematodes in goat faeces in the south-eastern United States: dose titration and dose time interval studies. Veterinary Parasitology 120 (2004) 285-296
26. Turenne C.Y., Sanche S.E., Hoban D.J., Karlowsky J.A., and Kabani A.J. Rapid identification of fungi by using the ITS2 genetic region and an automated fluorescent cappilary electrophoresis system. Journal of Clinical Microbiology 37 (1999) 1846-1851
27. Waller P.J., Larsen M., Faedo M., and Hennessy D.R. The potential of nematopaghous fungi to control the free-living stages of nemtode parasites of sheep: in vitro and in vivo studies. Veterinary Parasitology 51 (1994) 289-299
28. Waller P.J., Schwan O., Ljungstorm B.-L., Rydzik A., and Yeates G.W. Evaluation of biological control of sheep parasites using Duddingtonia flagrans under commercial farming conditions on the island of Gotland, Sweden. Veterinary Parasitology 126 (2004) 299-315