Detection of the nematophagous fungus Verticillium chlamydosporium in nematode-infested plant roots using PCR

Mycological Research

Volumn 104, Issue 4, 2000, Pages 435-439

  Hirsch, Penny R ^a   Mauchline, Tim H ^a   Mendum, Tom A ^a   Kerry, Brian R ^a  

^a ROTHAMSTED RESEARCH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BETA TUBULIN GENE; FUNGAL DNA; FUNGUS DETECTION; FUNGUS IDENTIFICATION; NEMATODE; POLYMERASE CHAIN REACTION; PRIMER DNA; RIBOSOMAL RNA; ROOT INFECTION; SEQUENCE HOMOLOGY; TOMATO;

DETECTION METHOD; FUNGUS;

ACREMONIUM; EPICHLOE; TRICHODERMA VIRIDE; VERTICILLIUM CHLAMYDOSPORIUM;

EID: 0034113362     PISSN: 09537562     EISSN: None     Source Type: Journal    
DOI: 10.1017/S0953756299001483     Document Type: Article

References (16)

1. Arora, D. K., Hirsch, P. R. & Kerry, B. R. (1996). PCR-based discrimination of Verticillium chlamydosporium isolates. Mycological Research 100, 801-809.
2. Bourne, J. M., Kerry, B. R. & de Leij, F. A. A. M. (1994). Methods for the study of Verticillium chlamydosporium in the rhizosphere. WORKSHOP: Organisms and Methods for Biological Control of Nematodes. Supplement to Journal of Nematology 26, 587-591.
3. Bourne, J. M., Kerry, B. R. & de Leij, F. A. A. M. (1996). The importance of the host plant on the interaction between root-knot nematodes (Meloidogyne spp.) and the nematophagous fungus. Verticillium chlamydosporium Goddard. Biocontrol Science and Technology 6, 539-548.
4. Byrd, A. D., Schardl, C. L., Songlin, P. J., Mogen, K. L. & Siegel, M. R. (1990). The beta-tubulin gene of Epichloë typhina from perennial ryegrass (Lolium perenne). Current Genetics 18, 347-354.
5. Cullen, D. W. & Hirsch, P. R. (1998). Simple and rapid method for direct extraction of microbial DNA from soil for PCR. Soil Biology Biochemistry 30, 983-993.
6. De Leij, F. A. A. M., Kerry, B. R. & Dennehy, J. A. (1993). Verticillium chlamydosporium as a biological control agent for Meloidogyne incognita and M. hapla in pot and micro-plot tests. Nematologica 39, 115-126.
7. Edlind, T, D., Li, J., Visvesvara, G. S., Vodkin, M. H., McLaughlin, G. L. & Katiyar, S. K. (1996). Phylogenetic analysis of β-tubulin sequences from a mitochondrial protozoa. Molecular Phylogenetics and Evolution 5, 359-367.
8. Gardes, M., White, T. J., Fortin, J. A., Bruns, T. D. & Taylor, J. W. (1991). Identification of indigenous and introduced symbiotic fungi in ectomycorrhizae by amplification of nuclear and mitochondrial ribosomal DNA. Canadian Journal of Botany 69, 180-190.
9. Goldman G. G. H., Temmerman, W. W., Jacobs, D. D., Contreras, R. R., Van Montagu, M. M. & Herrera-Estrella, A. A. (1993). A nucleotide substitution in one of the beta-tubulin genes of Trichoderma viride confers resistance to the antimitotic drug methyl benzimidazoie-2-yl-carbamate. Molecular & General Genetics 240, 73-80.
10. Irving, F. & Kerry, B. R. (1986). Variation between some strains of the nematophagous fungus, Verticillium chlamydosporium Goddard. II. Factors affecting parasitism of cyst nematode eggs. Nematologica 32, 474-485.
11. Liesack, W., Weyland, H. & Stackenbrandt, E. (1991). Potential risks of gene amplification by PCR as determined by 16S rDNA analysis of a mixed culture of strict barophilic bacteria. Microbial Ecology 21, 191-198.
12. Lovic, B. R., Valadez, V. A., Martyn, R. D. & Miller, M. E. (1995). Detection and identification of Monosporascus spp. with genus-specific PCR primers and nonradioactive hybridization probes. Plant Disease 79, 1169-1175.
13. Sherriff, C., Whelan, M. J., Arnold, G. M., Lafay, J.-F., Brygoo, Y. & Bailey. J.A. (1994). Ribosomal DNA sequence analysis reveals new species groupings in the genus Colletotrichum. Experimental Mycology 18. 121-138.
14. Thomas, W. B. (1996). Methyl bromide: effective pest management tool and environmental threat. Journal of Nematology 28, 586-589.
15. Tsai, H. F., Liu, J. S., Staben, C., Christensen, M. J., Latch, G. C., Siegel, M. R. & Schardl, C. L. (1994). Evolutionary diversification of fungal endophytes of tall fescue grass by hybridization with Epichloë species. Proceedings of the National Academy of Sciences, U.S.A. 91, 2542-2546.
16. White, T. J., Bruns, T., Lee, S. & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols (éd. M. A. Innes, D. H. Gelfand, J. S. Sninsky & T. J. White), pp. 315-322. Academic Press: London, U.K.