An improved microtitre enzyme immunoassay to measure the boar taint steroid 5α-androst-16-en-3-one in blood plasma of pigs

Meat Science

Volumn 80, Issue 3, 2008, Pages 934-938

  Claus, Rolf ^a   Lacorn, Markus ^a   Ostertag, Carmen ^a  

^a UNIVERSITY OF HOHENHEIM   (Germany)

Author keywords

Androstenone; Blood plasma; Enzyme immunoassay; Extraction; Pig

Indexed keywords

ARMORACIA RUSTICANA; BOVINAE; SUIDAE;

EID: 50249188265     PISSN: 03091740     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.meatsci.2008.04.019     Document Type: Article

References (24)

1. Andresen O. A radioimmunoassay for 5α-androst-16-en-3-one in porcine adipose tissue. Acta Endocrinologica 79 (1975) 619-624
2. Brooksbank B.W.L. Urinary excretion of androst-16-en-3α-ol levels in normal subjects, and effects of treatment with trophic hormones. Journal of Endocrinology 24 (1962) 435-444
3. Brooksbank B.W.L., and Haslewood G.A.D. The estimation of androst-16-en-3α-ol in human urine. Partial synthesis of androstenol and of its ß-glucosiduronic acid. Biochemical Journal 80 (1961) 488-496
4. Claus, R. (1970). Bestimmung von Testosteron und 5α-androst-16-en-3-on, einem Ebergeruchsstoff, bei Schweinen. Dissertation, Universität Weihenstephan.
5. Claus R. Dosage radioimmunologique du 5α-androst-16-en-3-one, stéroid responsable de l'odeur de verrat, dans le tissue adipeux des porcs. Comptes Reduce de Hebdomadeures de l'Academie des Sciences Paris 278 (1974) 299-302
6. Claus, R. (1979). Pheromone bei Säugetieren unter besonderer Berücksichtigung des Ebergeruchsstoffes und seiner Beziehung zu anderen Hodensteroiden. Beiheft 10: Fortschritte in der Tierphysiologie und Tierernährung. Paul Parey, Hamburg Berlin, pp. 1-136.
7. Claus R., and Alsing W. Occurence of 5α-androst-16-en-3-one, a boar pheromone in man and its relationship to testosterone. Journal of Endocrinology 68 (1976) 483-484
8. Claus R., Herbert E., and Dehnhard M. Comparative determination of the boar taint steroid androstenone in pig adipose tissue by a rapid enzyme immunoassay and an HPLC-method. Archiv für Lebensmittelhygiene 2 (1997) 27-30
9. Claus R., Lacorn M., Danowski K., Pearce M.C., and Bauer A. Short term endocrine and metabolic reactions before and after second immunization against GnRH in boars. Vaccine 25 (2007) 4689-4696
10. Claus R., Mahler G., and Münster E. Determination of the boar taint steroid 5α-androst-16-en-3-one in adipose tissue of pigs with a rapid microtitre plate enzyme-immunoassay (MTE). Archiv für Lebensmittelhygiene 4 (1988) 87-90
11. Claus R., and Schams D. Influence of mating and intrauterine oestradiol infusion on peripheral oxytocin concentrations in the sow. Journal of Endocrinology 126 (1990) 361-365
12. Edelhäuser M. Eine schnelle Methode zur Bestimmung des Ebergeruchssteroids Androstenon. Deutsche Lebensmittel-Rundschau 85 (1989) 80-84
13. Fuchs G. The correlation between the 5α-androst-16-en-3-one content and the sex odour intensity in boar fat. Swedish Journal of Agricultural Research 1 (1972) 233-237
14. Gower D.B. 16-Unsaturated C19 steroids: A review of their chemistry, biochemistry and possible physiological role. Journal of Steroid Biochemistry 3 (1972) 45-103
15. Hansen-Moeller J. Rapid high-performance liquid chromatographic method for simultaneous determination of androstenone, skatole and indole in back fat from pigs. Journal of Chromatography 661 (1994) 219-230
16. Kaufmann G., Ritter F., and Schubert K. Quantitative determination of the boar taint substance 5α-androst-16-en-3-one in fat. Journal of Steroid Biochemistry 7 (1976) 593-597
17. Melrose D.R., Reed H.C.B., and Patterson R.L.S. Androgen steroids associated with boar odour as an aid to the detection of oestrus in pig artificial insemination. British Veterinary Journal 127 (1971) 497-502
18. Metz C., Hohl K., Waidelich S., Drochner W., and Claus R. Active immunization of boars against GnRH at an early age: Consequences for testicular function, boar taint accumulation and N-retention. Livestock Production Science 74 (2002) 147-157
19. Patterson R.L.S. 5α-Androst-16-en-3-one: Compound responsible for taint in boar fat. Journal of Science of Food and Agriculture 19 (1968) 31-37
20. Prelog V., and Ruzicka L. 7. Untersuchungen über Organextrakte. Über zwei moschusartig riechende Steroide aus Schweinetestes-Extrakten. Helvetica Chimica Acta 27 (1944) 61-66
21. Ruokonen A. Free and sulphate conjugated 16-unsaturated C19 steroids in human testis tissue. Biochimica Biophysica Acta 316 (1973) 251-255
22. Ruzicka L., Prelog V., and Meister P. Steroide und Sexualhormone. 120. Mit.: Weitere Untersuchungen über den Zusammenhang zwischen Konstitution und Geruch bei Steroiden. Helvetica Chimica Acta 28 (1945) 1651-1660
23. Sinclair P.A., and Squires E.J. Testicular sulfoconjugation of the 16-androstenone steroids by hydroxysteroid sulfotransferase: Its effect on the concentrations of 5α-androstenone in plasma and fat of the mature domestic boar. Journal of Animal Science 83 (2005) 358-365
24. Squires E.J., and Lundström K. Relationship between cytochrome P450IIE1 in liver and levels of skatole and its metabolites in intact male pigs. Journal of Animal Science 75 (1997) 2506-2511