Generation and characterization of monoclonal antibodies against the proregion of human desmoglein-2

Hybridoma

Volumn 27, Issue 4, 2008, Pages 249-258

  Keim, Sarah A ^a   Johnson, Keith R ^a,b,c   Wheelock, Margaret J ^a,b,c,d   Wahl III, James K ^b,c  

^a UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

^b UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

^c UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

^d NEBRASKA MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DESMOGLEIN 2; DESMOSOMAL CADHERIN; MONOCLONAL ANTIBODY;

ARTICLE; BIOTINYLATION; CELL SURFACE; CONTROLLED STUDY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOPRECIPITATION; PRIORITY JOURNAL; PROTEIN ANALYSIS;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; CELL MEMBRANE; DESMOGLEIN 2; ENDOPLASMIC RETICULUM; FEMALE; GOLGI APPARATUS; HUMANS; MICE; MICE, INBRED BALB C; MODELS, BIOLOGICAL; PROTEIN STRUCTURE, TERTIARY; TISSUE DISTRIBUTION; TUMOR CELLS, CULTURED;

EID: 50249155884     PISSN: 15540014     EISSN: None     Source Type: Journal    
DOI: 10.1089/hyb.2008.0020     Document Type: Article

References (22)

1. Green KJ, and Simpson CL: Desmosomes: new perspectives on a classic. J Invest Dermatol 2007;127:2499-515.
2. Holthofer B, Windoffer R, Troyanovsky S, and Leube RE: Structure and function of desmosomes. Int Rev Cytol 2007;264:65-163.
3. Garrod DR, Merritt AJ, and Nie Z: Desmosomal cadherins. Curr Opin Cell Biol 2002;14:537-545.
4. Awad MM, Dalal D, Cho E, Amat-Alarcon N, James C, Tichnell C, Tucker A, Russell SD, Bluemke DA, Dietz HC, Calkins H, and Judge DP: DSG2 mutations contribute to arrhythmogenic right ventricular dysplasia/cardiomyopathy. Am J Hum Genet 2006;79:136-142.
5. Syed SE, Trinnaman B, Martin S, Major S, Hutchinson J, and Magee AI: Molecular interactions between desmosomal cadherins. Biochem J 2002;362:317-327.
6. Chitaev NA, and Troyanovsky SM: Direct Ca2+-dependent heterophilic interaction between desmosomal cadherins, desmoglein and desmocollin contributes to cell-cell adhesion. J Cell Biol 1997;138:193-201.
7. Nilles LA, Parry DA, Powers EE, Angst BD, Wagner RM, and Green KJ: Structural analysis and expression of human desmoglein: a cadherin-like component of the desmosome. J Cell Sci 1991;99 (Pt 4):809-821.
8. Getsios S, Huen AC, and Green KJ: Working out the strength and flexibility of desmosomes. Nat Rev Mol Cell Biol 2004;5:271-281.
9. Bryant DM, and Stow JL: The ins and outs of E-cadherin trafficking. Trends Cell Biol 2004;14:427-434.
10. Ozawa M, and Kemler R: Correct proteolytic cleavage is required for the cell adhesive function of uvomorulin. J Cell Biol 1990;111:1645-1650.
11. Wahl JK 3rd, Kim YJ, Cullen JM, Johnson KR, and Wheelock MJ: N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane. J Biol Chem 2003;278:17269-17276.
12. Wahl JK, Sacco PA, McGranahan-Sadler TM, Sauppé LM, Wheelock MJ, and Johnson KR: Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: identification of unique and shared domains. J Cell Sci 1996;109( Pt 5):1143-1154.
13. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-685.
14. Sheffield P, Garrard S, and Derewenda Z: Overcoming expression and purification problems of RhoGDI using a family of "parallel" expression vectors. Protein Expr Purif 1999;15:34-39.
15. Ireton RC, Davis MA, van Hengel J, Mariner DJ, Barnes K, Thoreson MA, Anastasiadis PZ, Matrisian L, Bundy LM, Sealy L, Gilbert B, van Roy F, and Reynolds AB: A novel role for p120 catenin in E-cadherin function. J Cell Biol 2002;159:465-476.
16. Wahl JK 3rd: Generation of monoclonal antibodies specific for desmoglein family members. Hybrid Hybridomics 2002;21:37-44.
17. Garrod D, and Kimura TE: Hyper-adhesion: a new concept in cell-cell adhesion. Biochem Soc Trans 2008;36:195-201.
18. Bech-Serra JJ, Santiago-Josefat B, Esselens C, Saftig P, Baselga J, Arribas J, and Canals F: Proteomic identification of desmoglein 2 and activated leukocyte cell adhesion molecule as substrates of ADAM17 and ADAM10 by difference gel electrophoresis. Mol Cell Biol 2006;26:5086-5095.
19. Posthaus H, Dubois CM, Laprise MH, Grondin F, Suter MM, and Müller E: Proprotein cleavage of E-cadherin by furin in baculovirus over-expression system: potential role of other convertases in mammalian cells. FEBS Lett 1998;438:306-310.
20. Seidah NG, Chretien M, and Day R: The family of subtilisin/kexin like pro-protein and pro-hormone convertases: divergent or shared functions. Biochimie 1994;76:197-209.
21. Nakayama K: Furin: a mammalian subtilisin/Kex2p-like endoprotease involved in processing of a wide variety of precursor proteins. Biochem J 1997;327( Pt 3):625-635.
22. Harrison OJ, Corps EM, Berge T, and Kilshaw PJ: The mechanism of cell adhesion by classical cadherins: the role of domain 1. J Cell Sci 2005;118:711-721.