Detection of viable Mycobacterium leprae in soil samples: Insights into possible sources of transmission of leprosy

Infection, Genetics and Evolution

Volumn 8, Issue 5, 2008, Pages 627-631

  Lavania, Mallika ^a   Katoch, Kiran ^a   Katoch, Vishwa Mohan ^a   Gupta, Anuj Kumar ^a   Chauhan, Devendra Singh ^a   Sharma, Rahul ^a   Gandhi, Rashi ^a   Chauhan, Varsha ^a   Bansal, Gurpreet ^a   Sachan, Pawan ^b   Sachan, Shailendra ^b   Yadav, V S ^a   Jadhav, Rupendra ^c  

^a NATIONAL JALMA INSTITUTE FOR LEPROSY AND OTHER MYCOBACTERIAL DISEASES   (India)

^b Model Rural Health Research Unit   (India)

^c The SBL ^*  (India)

Author keywords

(RT PCR); Leprosy; M. leprae; PCR; Reverse transcriptase; Soil

Indexed keywords

BACTERIAL DNA; BACTERIAL RNA; RNA 16S;

ARTICLE; BACTERIAL TRANSMISSION; BACTERIUM DETECTION; CONTROLLED STUDY; DNA DETERMINATION; DNA EXTRACTION; ENDEMIC DISEASE; GENE AMPLIFICATION; HUMAN; INFECTION RISK; LEPROSY; MYCOBACTERIUM LEPRAE; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RISK ASSESSMENT; RNA ANALYSIS; RNA EXTRACTION; SOIL ANALYSIS;

DNA, BACTERIAL; ENVIRONMENTAL MONITORING; LEPROSY; MYCOBACTERIUM LEPRAE; POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; SOIL MICROBIOLOGY;

ANIMALIA; BACILLI (CLASS); DASYPODIDAE; MYCOBACTERIUM LEPRAE;

EID: 50249153660     PISSN: 15671348     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.meegid.2008.05.007     Document Type: Article

References (18)

1. Alm E.W., and Stahl D.A. Critical factors influencing the recovery and integrity of rRNA extracted from environment samples use of an optimized protocol to measure depth related biomass distribution in fresh water sediments. J. Microbiol. Methods 40 (2000) 153-162
2. Desikan K.V. Viability of Mycobacterium leprae outside the human body. Lep. Rev. 48 (1977) 231-235
3. Desikan K.V., and Sreevasta. Extended studies on the viability of Mycobacterium leprae outside the human body. Lepr. Rev. 66 (1995) 287-295
4. Donoghue H.D., Holton J., and Spigelman M. PCR primers that can detect low levels of Mycobacterium leprae DNA. J. Med. Microbiol. 50 (2001) 177-182
5. Hellyer T.J., DesJardin L.E., Hehman G.L., Cave M.D., and Eisenach K.D. Quantitative analysis of mRNA as a marker for viability of Mycobacterium tuberculosis. J. Clin. Microbiol. 37 (1999) 290-295
6. Herrick J.B., Madsen E.L., Batt C.L., and Ghiorse W.C. Polymerase chain reaction amplification of napthalene-catabolic and 16S ribosomal-RNA gene-sequences from indigenous sediment bacteria. Appl. Environ. Microbiol. 59 (1993) 687-694
7. Holben W.E., Jansson J.K., Chelm B.K., and Tiedje J.M. DNA probe method for the detection of specific microorganisms in the soil bacterial community. Appl. Environ. Microbiol. 54 (1988) 703-711
8. Hurt R.A., Qiu X., Wu L., Roh Y., Palumbo A.V., Tiedje J.M., and Zhou J. Simultaneous recovery of RNA and DNA from soils and sediments. Appl. Environ. Microbiol. 67 (2001) 4495-4503
9. Jadhav R.S., Kamble R.R., Shinde V.S., Edward S., and Edward V.K. Use of reverse transcription polymerase chain reaction for the detection of Mycobacterium leprae in the slit-skin smears of leprosy patients. Indian J. Lepr. 77 (2005) 116-127
10. Jadin J.B. Amibes limax: vecteurs possibles de mycobactéries et de Mycobacterium leprae. Acta. Leprol. 59 (1975) 57-67
11. Kurabachew M., Wondimu A., and Ryon J.J. Reverse transcription-PCR detection of Mycobacterium leprae in clinical specimens. J. Clin. Microbiol. 36 (1998) 1352-1356
12. Lavania M., Katoch K., Sachan P., Dubey A., Kapoor S., Kashyap M., Chauhan D.S., Singh H.B., Sharma V.D., Katoch V.M., and Jadhav R.S. Detection of Mycobacterium leprae DNA from soil samples by PCR targeting RLEP sequences. J. Comm. Dis. 38 (2006) 69-73
13. Matsuoka M., Izumi S., Budiawan T., Nakata N., and Saeki K. Mycobacterium leprae DNA in daily using water as a possible source of leprosy infection. Indian J. Lepr. 71 (1999) 61-67
14. Miskin I.P., Farrimond P., and Head I.M. Identification of novel bacterial lineages as active members of microbial populations in a freshwater sediment using a rapid RNA extraction procedure and RT-PCR. Microbiology 145 (1999) 1977-1987
15. Phetsuksiri B., Rudeeaneksin J., Supapkul P., Wachapong S., Mahotarn K., and Brennan P.J. A simplified reverse transcriptase PCR for rapid detection of Mycobacterium leprae in skin specimens. FEMS Immunol. Med. Microbiol. 48 (2006) 319-328
16. Sharma R.K., Katoch K., Shivannavar C.T., Sharma V.D., Natrajan M., Bhatia A.S., Saxena N., and Katoch V.M. Detection of M. leprae by gene amplification; combined ethidium-bromide staining and probe hybridization. Int. J. Lepr. Other Mycobact. Dis. 64 (1996) 409-416
17. Somerville C.C., Knight I.T., Straube W.L., and Colwell R.R. Simple, rapid method for direct isolation of nucleic acids from aquatic environments. Appl. Environ. Microbiol. 55 (1989) 548-554
18. Tsai Y.L., and Olson B.H. Rapid method for separation of bacterial DNA from humic substrates in sediments for polymerase chain reaction. Appl. Environ. Microbiol. 58 (1992) 2292-2295