Rapid mass spectrometric analysis of 15N-Leu incorporation fidelity during preparation of specifically labeled NMR samples

Protein Science

Volumn 17, Issue 9, 2008, Pages 1636-1639

  Truhlar, Stephanie M E ^a   Cervantes, Carla F ^a   Torpey, Justin W ^a   Kjaergaard, Magnus ^b   Komives, Elizabeth A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

MALDI mass spectrometry; NMR spectroscopy; Proteolytic digestion; Specific 15N amino acid labeling; Tandem mass spectrometry

Indexed keywords

AMINO ACID; LEUCINE; NITROGEN 15;

AMINO ACID ANALYSIS; ANALYTIC METHOD; ARTICLE; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; ANKYRIN REPEAT; ELECTRON PROBE MICROANALYSIS; HYDROLYSIS; I-KAPPA B KINASE; ISOTOPE LABELING; LEUCINE; MOLECULAR SEQUENCE DATA; MUTATION; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PEPTIDES; PROTEIN STRUCTURE, TERTIARY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TANDEM MASS SPECTROMETRY; TIME FACTORS;

EID: 50049129937     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.036418.108     Document Type: Article

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