Interaction of CheY2 and CheY2-P with the cognate CheA kinase in the chemosensory-signalling chain of Sinorhizobium meliloti

Molecular Microbiology

Volumn 69, Issue 6, 2008, Pages 1373-1384

  Riepl, Hubert ^a   Maurer, Till ^a   Kalbitzer, Hans Robert ^a   Meier, Veronika M ^a   Haslbeck, Martin ^b   Schmitt, Rüdiger ^a   Scharf, Birgit ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CHEA KINASE; CHEY PROTEIN; CHEY2 P PROTEIN; CHEY2 PROTEIN; PROTEIN HISTIDINE KINASE; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CHEMOSENSITIZATION; CHEY1 GENE; CHEY2 GENE; CHEZ GENE; CONTROLLED STUDY; DISSOCIATION CONSTANT; ENZYME ACTIVE SITE; FLAGELLUM; LIGAND BINDING; MOTOR CONTROL; NONHUMAN; PHOSPHATE TRANSPORT; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; REGULATOR GENE; REGULATORY MECHANISM; SINORHIZOBIUM MELILOTI;

AMINO ACID SEQUENCE; ARTIFICIAL GENE FUSION; BACTERIAL PROTEINS; CHEMOTAXIS; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; KINETICS; MODELS, BIOLOGICAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN INTERACTION MAPPING; PROTEIN KINASES; SINORHIZOBIUM MELILOTI; TRANSCRIPTION FACTORS;

SINORHIZOBIUM MELILOTI;

EID: 50049114701     PISSN: 0950382X     EISSN: 13652958     Source Type: Journal    
DOI: 10.1111/j.1365-2958.2008.06342.x     Document Type: Article

References (45)

1. Alley, M.R.K., Maddock, J.R. Shapiro, L. (1992) Polar localization of a bacterial chemoreceptor. Genes Dev 6 : 825 836.
2. Armitage, J.P. Schmitt, R. (1997) Bacterial chemotaxis: Rhodobacter sphaeroides and Sinorhizobium meliloti- variations on a theme? Microbiology 143 : 3671 3682.
3. Bachmann, B.J. (1990) Linkage map of Escherichia coli K-12, edition 8. Microbiol Rev 54 : 130 197. [Published erratum appears in Microbiol Rev (1991) 55: 191.]
4. Blat, Y. Eisenbach, M. (1994) Phosphorylation-dependent binding of the chemotaxis signal molecule CheY to its phosphatase, CheZ. Biochemistry 33 : 902 906.
5. Cho, H.S., Lee, S.Y., Yan, D., Pan, X., Parkinson, J.S., Kustu, S., et al. (2000) NMR structure of activated CheY. J Mol Biol 297 : 543 551.
6. Gegner, J.A. Dahlquist, F.W. (1991) Signal transduction in bacteria: CheW forms a reversible complex with the protein kinase CheA. Proc Natl Acad Sci USA 88 : 750 754.
7. Gestwicki, J.E., Lamanna, A.C., Harshey, R.M., McCarter, L.L., Kiessling, L.L. Adler, J. (2000) Evolutionary conservation of methyl-accepting chemotaxis protein location in Bacteria and Archaea. J Bacteriol 182 : 6499 6502.
8. Götz, R., Limmer, N., Ober, K. Schmitt, R. (1982) Motility and chemotaxis in two strains of Rhizobium with complex flagella. J Gen Microbiol 128 : 789 798.
9. 124-257 complex. Acta Cryst 57 : 44 51.
10. Greck, M., Platzer, J., Sourjik, V. Schmitt, R. (1995) Analysis of a chemotaxis operon in Rhizobium meliloti. Mol Microbiol 15 : 989 1000.
11. Grossmann, G., Opekarova, M., Malinsky, J., Weig-Meckl, I. Tanner, W. (2007) Membrane potential governs lateral segregation of plasma membrane proteins and lipids in yeast. EMBO J 26 : 1 8.
12. Harrison, D.M., Skidmore, J., Armitage, J.P. Maddock, J.R. (1999) Localization and environmental regulation of MCP-like proteins in Rhodobacter sphaeroides. Mol Microbiol 31 : 885 892.
13. Hess, J.F., Oosawa, K., Kaplan, N. Simon, M.I. (1988) Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxis. Cell 53 : 79 87.
14. Higuchi, R. (1989) Using PCR to engineer DNA. In PCR Technology. Principles and Applications for DNA Amplification. Ehrlich, H.A. (ed.). New York : Stockton Press, pp. 61 70.
15. Kamberger, W. (1979) An Ouchterlony double diffusion study on the interaction between legume lectins and rhizobial cell surface antigens. Arch Microbiol 121 : 83 90.
16. Koradi, R., Billeter, M. Wüthrich, K. (1996) molmol: a program for display and analysis of macromolecular structures. J Mol Graph 14 : 51 55.
17. Li, J., Swanson, R.V., Simon, M.I. Weis, R.M. (1995) The response regulators CheB and CheY exhibit competitive binding to the kinase CheA. Biochemistry 34 : 14626 14636.
18. Luria, S.E., Adams, F.N. Ting, R.C. (1960) Transduction of lactose utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles. Virology 12 : 348 390.
19. McEvoy, M.M., Hausrath, A.C., Randolph, G.B., Remington, S.J. Dahlquist, F.W. (1998) Two binding modes reveal flexibility in kinase/response regulator interactions in the bacterial chemotaxis pathway. Proc Natl Acad Sci USA 95 : 7333 7338.
20. Maddock, J.R. Shapiro, L. (1993) Polar location of the chemoreceptor complex in the Escherichia coli cell. Science 259 : 1717 1723.
21. Meier, V.M., Muschler, P. Scharf, B.E. (2007) Functional analysis of nine putative chemoreceptor proteins in Sinorhizobium meliloti. J Bacteriol 189 : 1816 1826.
22. Neidig, K.P., Geyer, M., Görler, A., Antz, C., Saffrich, R., Beneicke, W. Kalbitzer, H.R. (1995) aurelia, a program for computer-aided analysis of multidimensional NMR spectra. J Biomol NMR 6 : 255 270.
23. Novick, R.P., Clowes, R.C., Cohen, S.N., Curtiss, R., 3rd, Datta, N. Falkow, S. (1976) Uniform nomenclature for bacterial plasmids: a proposal. Bacteriol Rev 40 : 168 189.
24. Platzer, J., Sterr, W., Hausmann, M. Schmitt, R. (1997) Three genes of a motility operon and their role in flagellar rotary speed variation in Rhizobium meliloti. J Bacteriol 179 : 6391 6399.
25. Riepl, H. (2003) NMR-spektroskopische Charakterisierung des Responsregulators CheY2 aus Sinorhizobium meliloti. PhD Thesis. University of Regensburg.
26. Riepl, H., Scharf, B., Schmitt, R., Kalbitzer, H.R. Maurer, T. (2001) Sequential assignment and secondary structure of the 14 kDa chemotactic protein CheY2 from Sinorhizobium meliloti. J Biomol NMR 19 : 287 288.
27. 3-activated response regulator CheY2. J Mol Biol 338 : 287 297.
28. Scharf, B., Schuster-Wolff-Bühring, H., Rachel, R. Schmitt, R. (2001) Mutational analysis of Rhizobium lupini H13-3 and Sinorhizobium meliloti flagellin genes: importance of flagellin A for flagellar filament structure and transcriptional regulation. J Bacteriol 183 : 5334 5342.
29. Schleucher, J., Schwendinger, M., Sattler, M., Schmidt, P., Schedletzky, O., Glaser, S.J., et al. (1994) A general enhancement scheme in heteronuclear multidimensional NMR employing pulsed field gradients. J Biomol NMR 4 : 301 306.
30. Schmitt, R. (2002) Sinorhizobial chemotaxis: a departure from the enterobacterial paradigm. Microbiology 148 : 627 631.
31. - bacteria using a genetically modified sacRB gene as positive selection marker. Appl Microbiol Biotechnol 38 : 615 618.
32. Simon, R., O'Connell, M., Labes, M. Pühler, A. (1986) Plasmid vectors for the genetic analysis and manipulation of rhizobia and other gram-negative bacteria. Methods Enzymol 118 : 640 659.
33. Skidmore, J.M., Ellefson, D.D., McNamara, B.P., Couto, M.M., Wolfe, A.J. Maddock, J.R. (2000) Polar clustering of the chemoreceptor complex in Escherichia coli occurs in the absence of complete CheA function. J Bacteriol 182 : 967 973.
34. Sourjik, V. Schmitt, R. (1996) Different roles of CheY1 and CheY2 in the chemotaxis of Rhizobium meliloti. Mol Microbiol 22 : 427 436.
35. Sourjik, V. Schmitt, R. (1998) Phosphotransfer between CheA, CheY1, and CheY2 in the chemotaxis signal transduction chain of Rhizobium meliloti. Biochemistry 37 : 2327 2335.
36. Stewart, R.C. (1997) Kinetic characterization of phosphotransfer between CheA and CheY in the bacterial chemotaxis signal transduction pathway. Biochemistry 36 : 2030 2040.
37. Stewart, R.C., Jahreis, K. Parkinson, J.S. (2000) Rapid phosphotransfer to CheY from a CheA protein lacking the CheY-binding domain. Biochemistry 39 : 13157 13165.
38. Stock, J.B. Surette, M.G. (1996) Chemotaxis. In Escherichia coli and Salmonella, Vol. I. Neidhardt, F.C. (ed.). Washington, DC : American Society for Microbiology Press, pp. 1103 1129.
39. Stock, A., Chen, T., Welsh, D. Stock, J. (1988) CheA protein, a central regulator of bacterial chemotaxis, belongs to a family of proteins that control gene expression in response to changing environmental conditions. Proc Natl Acad Sci USA 85 : 1403 1407.
40. Swanson, R.V., Schuster, S.C. Simon, M.I. (1993) Expression of CheA fragments which define domains encoding kinase, phosphotransfer, and CheY binding activities. Biochemistry 32 : 7623 7629.
41. Welch, M., Oosawa, K., Aizawa, S. Eisenbach, M. (1993) Phosphorylation-dependent binding of a signal molecule to the flagellar switch of bacteria. Proc Natl Acad Sci USA 90 : 8787 8791.
42. Welch, M., Chinardet, N., Mourey, L., Birck, C. Samama, J.-P. (1998) Structure of the CheY-binding domain of histidine kinase CheA in complex with CheY. Nat Struct Biol 5 : 25 29.
43. Wishart, D.S. Sykes, B.D. (1994) The 13C chemical-shift index: a simple method for the identification of protein secondary structure using 13C chemical-shift data. J Biomol NMR 4 : 171 180.
44. Yan, D., Cho, H.S., Hastings, C.A., Igo, M.M., Lee, S.Y., Pelton, J.G., et al. (1999) Beryllofluoride mimics phosphorylation of NtrC and other bacterial response regulators. Proc Natl Acad Sci USA 96 : 14789 14794.
45. Zhu, X., Rebello, J., Matsumura, P. Volz, K. (1997) Crystal structures of CheY mutants Y106W and T87I/Y106W. CheY activation correlates with movement of residue 106. J Biol Chem 272 : 5000 5006.