Polar clustering of the chemoreceptor complex in Escherichia coli occurs in the absence of complete CheA function

Journal of Bacteriology

Volumn 182, Issue 4, 2000, Pages 967-973

  Skidmore, J M ^a   Ellefson, D D ^b,c   Mcnamara, B P ^b,d   Couto, M M P ^a   Wolfe, A J ^b   Maddock, J R ^a  

^a UNIVERSITY OF MICHIGAN   (United States)

^b LOYOLA UNIVERSITY CHICAGO   (United States)

^c OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

^d UNIVERSITY OF MARYLAND   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL AGGREGATION; CELL MOTILITY; CHEMOKINESIS; CHEMORECEPTOR; ESCHERICHIA COLI; FLAGELLUM; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; STRAIN DIFFERENCE;

ALLELES; AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; CHEMOTAXIS; ESCHERICHIA COLI; MEMBRANE PROTEINS; MICROSCOPY, IMMUNOELECTRON; MOVEMENT; PLASMIDS; VARIATION (GENETICS);

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0033979479     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.182.4.967-973.2000     Document Type: Article

References (41)

1. Alley, M. R., J. R. Maddock, and L. Shapiro. 1992. Polar localization of a bacterial chemoreceptor. Genes Dev. 6:825-836.
2. Ames, P., and J. S. Parkinson. 1994. Constitutively signaling fragments of Tsr, the Escherichia coli serine chemoreceptor. J. Bacteriol. 176:6340-6348.
3. Berg, H. C., and L. Turner. 1995. Cells of Escherichia coli swim either end forward. Proc. Natl. Acad. Sci. USA 92:477-479.
4. Bilwes, A. M., L. A. Alex, B. R. Crane, and M. I. Simon. 1999. Structure of CheA, a signal-transducing histidine kinase. Cell 96:131-141.
5. Borkovich, K. A., N. Kaplan, J. F. Hess, and M. I. Simon. 1989. Transmembrane signal transduction in bacterial chemotaxis involves ligand-dependent activation of phosphate group transfer. Proc. Natl. Acad. Sci. USA 86:1208-1212.
6. Borkovich, K. A., and M. I. Simon. 1990. The dynamics of protein phosphorylation in bacterial chemotaxis. Cell 63:1339-1348.
7. Bourret, R. B., J. Davagnino, and M. I. Simon. 1993. The carboxy-terminal portion of the CheA kinase mediates regulation of autophosphorylation by transducer and CheW. J. Bacteriol. 175:2097-2101.
8. Bray, D., M. D. Levin, and C. J. Morton-Firth. 1998. Receptor clustering as a cellular mechanism to control sensitivity. Nature 393:85-88.
9. Ellefson, D. D., U. Weber, and A. J. Wolfe. 1997. Genetic analysis of the catalytic domain of the chemotaxis-associated histidine kinase CheA. J. Bacteriol. 179:825-830.
10. Gegner, J. A., D. R. Graham, A. F. Roth, and F. W. Dahlquist. 1992. Assembly of an MCP receptor, CheW, and kinase CheA complex in the bacterial chemotaxis signal transduction pathway. Cell 70:975-982.
11. Harrison, D. M., J. Skidmore, J. P. Armitage, and J. R. Maddock. 1999. Localization and environmental regulation of MCP-like proteins in Rhodobacter sphaeroides. Mol. Microbiol. 31:885-892.
12. Hess, J. F., R. B. Bourret, and M. I. Simon. 1988. Histidine phosphorylation and phosphoryl group transfer in bacterial chemotaxis. Nature 336:139-143.
13. Hess, J. F., K. Oosawa, N. Kaplan, and M. I. Simon. 1988. Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxis. Cell 53:79-87.
14. Kofoid, E. C., and J. S. Parkinson. 1991. Tandem translation starts in the cheA locus of Escherichia coli. J. Bacteriol. 173:2116-2119.
15. Kunkel, T. A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82:488-492.
16. Lee, N., J. Liu, C. He, and D. Testa. 1991. Site-specific mutagenesis method which completely excludes wild-type DNA from the transformants. Appl. Environ. Microbiol. 57:2888-2890.
17. Liu, Y., M. Levit, R. Lurz, M. G. Surette, and J. B. Stock. 1997. Receptor-mediated protein kinase activation and the mechanism of transmembrane signaling in bacterial chemotaxis. EMBO J. 16:7231-7240.
18. Lybarger, S. R., and J. R. Maddock. 1999. Clustering of the chemoreceptor complex in Escherichia coli is independent of the methyltransferase CheR and the methylesterase CheB. J. Bacteriol. 181:5527-5529.
19. Maddock, J. R., and L. Shapiro. 1993. Polar location of the chemoreceptor complex in the Escherichia coli cell. Science 259:1717-1723.
20. Manson, M. D., J. P. Armitage, J. A. Hoch, and R. M. Macnab. 1998. Bacterial locomotion and signal transduction. J. Bacteriol. 180:1009-1022.
21. Matsumura, P., S. Roman, K. Volz, and D. McNally. 1990. Signalling complexes in bacterial chemotaxis. Symp. Soc. Gen. Microbiol. 46:135-154.
22. McNally, D. F., and P. Matsumura. 1991. Bacterial chemotaxis signaling complexes: formation of a CheA/CheW complex enhances autophosphorylation and affinity for CheY. Proc. Natl. Acad. Sci. USA 88:6269-6273.
23. McNamara, B. P., and A. J. Wolfe. 1997. Coexpression of the long and short forms of CheA, the chemotaxis histidine kinase, by members of the family Enterobacteriaceae. J. Bacteriol. 179:1813-1818.
24. Morrison, T. B., and J. S. Parkinson. 1994. Liberation of an interaction domain from the phosphotransfer region of CheA, a signaling kinase of Escherichia coli. Proc. Natl. Acad. Sci. USA 91:5485-5489.
25. Ninfa, E. G., A. Stock, S. Mowbray, and J. Stock. 1991. Reconstitution of the bacterial chemotaxis signal transduction system from purified components. J. Biol. Chem. 266:9764-9770.
26. Oosawa, K., N. Muoh, and M. I. Simon. 1988. Cloning of the C-terminal cytoplasmic fragment of the Tar protein and effects of the fragment on chemotaxis of Escherichia coli. J. Bacteriol. 170:2521-2526.
27. Park, C., and G. L. Hazelbauer. 1986. Mutations specifically affecting ligand interaction of the Trg chemosensory transducer. J. Bacteriol. 167:101-109.
28. Parkinson, J. S., and S. E. Houts. 1982. Isolation and behavior of Escherichia coli deletion mutants lacking chemotaxis functions. J. Bacteriol. 151:106-113.
29. Parkinson, J. S., and E. C. Kofoid. 1992. Communication modules in bacterial signaling proteins. Annu. Rev. Genet. 26:71-112.
30. Sanatinia, H., E. C. Kofoid, T. B. Morrison, and J. S. Parkinson. 1995. The smaller of two overlapping cheA gene products is not essential for chemotaxis in Escherichia coli. J. Bacteriol. 177:2713-2720.
31. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
32. Silhavy, T. J., M. L. Berman, and L. W. Enquist. 1984. Experiments with gene fusions. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
33. Smith, R. A., and J. S. Parkinson. 1980. Overlapping genes at the cheA locus of Escherichia coli. Proc. Natl. Acad. Sci. USA 77:5370-5374.
34. Stewart, R. C., R. VanBruggen, D. D. Ellefson, and A. J. Wolfe. 1998. TNP-ATP and TNP-ADP as probes of the nucleotide binding site of CheA, the histidine protein kinase in the chemotaxis signal transduction pathway of Escherichia coli. Biochemistry 37:12269-12279.
35. Swanson, R. V., R. B. Bourret, and M. I. Simon. 1993. Intermolecular complementation of the kinase activity of CheA. Mol. Microbiol. 8:435-441.
36. Swanson, R. V., S. C. Schuster, and M. I. Simon. 1993. Expression of CheA fragments which define domains encoding kinase, phosphotransfer, and CheY binding activities. Biochemistry 32:7623-7629.
37. Wang, H., and P. Matsumura. 1996. Characterization of the CheAS/CheZ complex: a specific interaction resulting in enhanced dephosphorylating activity on CheY-phosphate. Mol. Microbiol. 19:695-703.
38. Wang, H., and P. Matsumura. 1997. Phosphorylating and dephosphorylating protein complexes in bacterial chemotaxis. J. Bacteriol. 179:287-289.
39. Wolfe, A. J., and H. C. Berg. 1989. Migration of bacteria in semisolid agar. Proc. Natl. Acad. Sci. USA 86:6973-6977.
40. Wolfe, A. J., B. P. McNamara, and R. C. Stewart. 1994. The short form of CheA couples chemoreception to CheA phosphorylation. J. Bacteriol. 176: 4483-4491.
41. Wolfe, A. J., and R. C. Stewart. 1993. The short form of the CheA protein restores kinase activity and chemotactic ability to kinase-deficient mutants. Proc. Natl. Acad. Sci. USA 90:1518-1522.