Automated analysis of two- and three-color fluorescent Elispot (Fluorospot) assays for cytokine secretion

Computer Methods and Programs in Biomedicine

Volumn 92, Issue 1, 2008, Pages 54-65

  Rebhahn, Jonathan A ^a   Bishop, Courtney ^a   Divekar, Anagha A ^a   Jiminez Garcia, Katty ^a   Kobie, James J ^a   Lee, F Eun Hyung ^a   Maupin, Genny M ^a   Snyder Cappione, Jennifer E ^b   Zaiss, Dietmar M ^c   Mosmann, Tim R ^a  

^a UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c Department of Infectious Diseases and Immunology   (Netherlands)

Author keywords

Cytokines; Elispot; Exploraspot; Fluorescence; Fluorospot; Imaging; Multicolor

Indexed keywords

ASSAYS; AUTOMATION; CELLS; CHLORINE COMPOUNDS; FLOW CYTOMETRY; FLUORESCENCE; IMAGING TECHNIQUES; LIGHT EMISSION; LUMINESCENCE; MICROSCOPIC EXAMINATION; PARAMETER ESTIMATION;

AUTOMATED ANALYSIS; AUTOMATED COUNTING; COLOR MEASUREMENTS; CYTOKINE SECRETIONS; CYTOKINES; DATA COMPLEXITY; ELISPOT; EXPLORASPOT; FLOW CYTOMETRY ANALYSIS; FLUORESCENCE MICROSCOPE IMAGES; FLUOROSPOT; GRAPHICAL DISPLAYS; HUMAN CYTOKINE; IMAGING; LOW FREQUENCIES; MORPHOLOGICAL PARAMETERS; MULTICOLOR; T CELLS;

CYTOLOGY;

CD4 ANTIGEN; CD8 ANTIGEN; CYTOKINE; GAMMA INTERFERON; INTERLEUKIN 2; MACROPHAGE INFLAMMATORY PROTEIN 1ALPHA;

ANIMAL CELL; ARTICLE; AUTOMATION; CD4+ T LYMPHOCYTE; CONTROLLED STUDY; CYTOKINE RELEASE; ENZYME LINKED IMMUNOSPOT ASSAY; EXPLORASPOT ASSAY; FLOW CYTOMETRY; FLUORESCENCE; FLUOROSPOT ASSAY; HUMAN; HUMAN CELL; IMAGE ANALYSIS; MOUSE; NONHUMAN; PHENOTYPE;

ALGORITHMS; ANIMALS; ARTIFICIAL INTELLIGENCE; CELLS, CULTURED; CYTOKINES; GENE EXPRESSION PROFILING; IMAGE INTERPRETATION, COMPUTER-ASSISTED; LYMPHOCYTES; MICE; MICROSCOPY, FLUORESCENCE, MULTIPHOTON; PATTERN RECOGNITION, AUTOMATED;

EID: 49949106240     PISSN: 01692607     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cmpb.2008.06.002     Document Type: Article

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