Detection of N-acyl homoserine lactones using a traI-luxCDABE-based biosensor as a high-throughput screening tool

BMC Biotechnology

Volumn 8, Issue , 2008, Pages

  Bernier, Steve P ^a,b   Beeston, Anne L ^a,c   Sokol, Pamela A ^a  

^a UNIVERSITY OF CALGARY   (Canada)

^b INSTITUT PASTEUR   (France)

^c CANADIAN FOOD INSPECTION AGENCY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ARSENIC COMPOUNDS; BACTERIOLOGY; BIOSENSORS; CHLORINE COMPOUNDS; COMPLEXATION; POLYSACCHARIDES; SIGNAL TRANSDUCTION; SYNTHESIS (CHEMICAL); THROUGHPUT;

A-DENSITY; ACYL HOMOSERINE LACTONE; BACTERIAL SPECIES; BIO SENSOR; BURKHOLDERIA; CO CULTURES; CO-CULTURE SYSTEM; GRAM-NEGATIVE SPECIES; HIGH THROUGHPUT SCREENINGS; HIGH THROUGHPUTS; N-ACYL HOMOSERINE LACTONES; OVER GROWTH; P. AERUGINOSA; PSEUDOMONAS AERUGINOSA; REAL TIME;

ASSAYS;

N ACYLHOMOSERINE LACTONE; BACTERIAL PROTEIN; GAMMA BUTYROLACTONE DERIVATIVE; TRAR PROTEIN, AGROBACTERIUM TUMEFACIENS;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BIOLUMINESCENCE; BIOSENSOR; BURKHOLDERIA CEPACIA; COCULTURE; CONTROLLED STUDY; GENE EXPRESSION REGULATION; GRAM NEGATIVE BACTERIUM; HIGH THROUGHPUT SCREENING; LIQUID CULTURE; NONHUMAN; OPERON; PSEUDOMONAS AERUGINOSA; QUORUM SENSING; RHIZOBIUM RADIOBACTER; BACTERIAL GENE; GENETIC PROCEDURES; GENETICS; LUMINESCENCE; METABOLISM; PLASMID; PROMOTER REGION; REPORTER GENE; TIME;

BACTERIA (MICROORGANISMS); BURKHOLDERIA CENOCEPACIA; NEGIBACTERIA; PSEUDOMONAS AERUGINOSA;

ACYL-BUTYROLACTONES; BACTERIAL PROTEINS; BIOSENSING TECHNIQUES; BURKHOLDERIA CEPACIA; GENES, BACTERIAL; GENES, REPORTER; LUMINESCENT MEASUREMENTS; OPERON; PLASMIDS; PROMOTER REGIONS (GENETICS); PSEUDOMONAS AERUGINOSA; RHIZOBIUM RADIOBACTER; TIME FACTORS;

EID: 49949085768     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-8-59     Document Type: Article

References (25)

1. Fuqua WC Winans SC Greenberg EP Quorum sensing in bacteria: The LuxR-LuxI family of cell density-responsive transcriptional regulators J Bacteriol 1994, 176(2):269-275. 205046 8288518
2. Engebrecht J Silverman M Identification of genes and gene products necessary for bacterial bioluminescence Proc Natl Acad Sci U S A 1984, 81(13):4154-4158. 345387 6377310 10.1073/pnas.81.13.4154
3. Whitehead NA Barnard AM Slater H Simpson NJ Salmond GP Quorum-sensing in Gram-negative bacteria FEMS Microbiol Rev 2001, 25(4):365-404. 10.1111/ j.1574-6976.2001.tb00583.x 11524130
4. Steindler L Venturi V Detection of quorum-sensing N-acyl homoserine lactone signal molecules by bacterial biosensors FEMS Microbiol Lett 2007, 266(1):1-9. 10.1111/j.1574-6968.2006.00501.x 17233715
5. Sokol PA Sajjan U Visser MB Gingues S Forstner J Kooi C The CepIR quorum-sensing system contributes to the virulence of Burkholderia cenocepacia respiratory infections Microbiology 2003, 149(Pt 12):3649-3658. 10.1099/mic.0.26540-0 14663096
6. Chambers CE Visser MB Schwab U Sokol PA Identification of N-acylhomoserine lactones in mucopurulent respiratory secretions from cystic fibrosis patients FEMS Microbiol Lett 2005, 244(2):297-304. 10.1016/j.femsle.2005.01.055 15766782
7. Watson B Currier TC Gordon MP Chilton MD Nester EW Plasmid required for virulence of Agrobacterium tumefaciens J Bacteriol 1975, 123(1):255-264. 235714 1141196
8. Zhu J Beaber JW More MI Fuqua C Eberhard A Winans SC Analogs of the autoinducer 3-oxooctanoyl-homoserine lactone strongly inhibit activity of the TraR protein of Agrobacterium tumefaciens J Bacteriol 1998, 180(20):5398-5405. 107588 9765571
9. Meighen EA Szittner RB Multiple repetitive elements and organization of the lux operons of luminescent terrestrial bacteria J Bacteriol 1992, 174(16):5371-5381. 206375 1644764
10. Sokol PA Malott RJ Riedel K Eberl L Communication systems in the genus Burkholderia: Global regulators and targets for novel antipathogenic drugs Future Microbiol 2007, 2(5):555-563. 10.2217/17460913.2.5.555 17927476
11. Lewenza S Conway B Greenberg EP Sokol PA Quorum sensing in Burkholderia cepacia: Identification of the LuxRI homologs CepRI J Bacteriol 1999, 181(3):748-756. 93439 9922236
12. Lewenza S Sokol PA Regulation of ornibactin biosynthesis and N-acyl-L-homoserine lactone production by CepR in Burkholderia cepacia J Bacteriol 2001, 183(7):2212-2218. 95126 11244059 10.1128/ JB.183.7.2212-2218.2001
13. Malott RJ Baldwin A Mahenthiralingam E Sokol PA Characterization of the cciIR quorum-sensing system in Burkholderia cenocepacia Infect Immun 2005, 73(8):4982-4992. 1201253 16041013 10.1128/IAI.73.8.4982-4992.2005
14. Juhas M Eberl L Tummler B Quorum sensing: The power of cooperation in the world of Pseudomonas Environ Microbiol 2005, 7(4):459-471. 10.1111/ j.1462-2920.2005.00769.x 15816912
15. Pearson JP Gray KM Passador L Tucker KD Eberhard A Iglewski BH Greenberg EP Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes Proc Natl Acad Sci U S A 1994, 91(1):197-201. 42913 8278364 10.1073/pnas.91.1.197
16. Winson MK Camara M Latifi A Foglino M Chhabra SR Daykin M Bally M Chapon V Salmond GP Bycroft BW Lazdunski A Stewart GSAB Williams P Multiple N-acyl-L-homoserine lactone signal molecules regulate production of virulence determinants and secondary metabolites in Pseudomonas aeruginosa Proc Natl Acad Sci U S A 1995, 92(20):9427-9431. 40998 7568146 10.1073/pnas.92.20.9427
17. Pesci EC Pearson JP Seed PC Iglewski BH Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa J Bacteriol 1997, 179(10):3127-3132. 179088 9150205
18. Henry DA Campbell ME LiPuma JJ Speert DP Identification of Burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple new selective medium J Clin Microbiol 1997, 35(3):614-619. 229637 9041399
19. Bernier SP Nguyen DT Sokol PA A LysR-type transcriptional regulator in Burkholderia cenocepacia influences colony morphology and virulence Infect Immun 2008, 76(1):38-47. 2223663 17967860 10.1128/IAI.00874-07
20. Cegelski L Marshall GR Eldridge GR Hultgren SJ The biology and future prospects of antivirulence therapies Nature reviews 2008, 6(1):17-27. 2211378 18079741 10.1038/nrmicro1818
21. Mahenthiralingam E Coenye T Chung JW Speert DP Govan JR Taylor P Vandamme P Diagnostically and experimentally useful panel of strains from the Burkholderia cepacia complex J Clin Microbiol 2000, 38(2):910-913. 86244 10655415
22. Holloway BW Romling U Tummler B Genomic mapping of Pseudomonas aeruginosa PAO Microbiology 1994, 140(Pt 11):2907-2929. 7812433
23. Brint JM Ohman DE Synthesis of multiple exoproducts in Pseudomonas aeruginosa is under the control of RhlR-RhlI, another set of regulators in strain PAO1 with homology to the autoinducer-responsive LuxR-LuxI family J Bacteriol 1995, 177(24):7155-7163. 177595 8522523
24. Gambello MJ Iglewski BH Cloning and characterization of the Pseudomonas aeruginosa lasR gene, a transcriptional activator of elastase expression J Bacteriol 1991, 173(9):3000-3009. 207884 1902216
25. Pearson JP Pesci EC Iglewski BH Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes J Bacteriol 1997, 179(18):5756-5767. 179464 9294432