Western-blot detection of RickA within spotted fever group rickettsiae using a specific monoclonal antibody

FEMS Microbiology Letters

Volumn 286, Issue 2, 2008, Pages 257-262

  Balraj, Premanand ^a   Nappez, Claude ^a   Raoult, Didier ^a   Renesto, Patricia ^a  

^a AIX MARSEILLE UNIVERSITÉ   (France)

Author keywords

Actin based motility; Monoclonal antibody; RickA; Rickettsiae

Indexed keywords

BACTERIAL PROTEIN; MONOCLONAL ANTIBODY; RICKA PROTEIN;

ARTICLE; BACTERIUM CULTURE; CELL SPREADING; FEVER; MASS SPECTROMETRY; NUCLEOTIDE SEQUENCE; PHENOTYPE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; RICKETTSIA CONORII; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, MONOCLONAL; BACTERIAL PROTEINS; BLOTTING, WESTERN; DNA, BACTERIAL; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FEMALE; MASS SPECTROMETRY; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; RICKETTSIA; SEQUENCE ANALYSIS, DNA;

RICKETTSIA CONORII; RICKETTSIACEAE;

EID: 49749142339     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2008.01283.x     Document Type: Article

References (27)

1. Andersson SG, Zomorodipour A, Andersson JO et al 1998) The genome sequence of Rickettsia prowazekii and the origin of mitochondria. Nature 396: 133 140.
2. Balraj P, El Karkouri K, Vestris G, Espinosa L, Raoult D Renesto P (2008) RickA expression is not sufficient to promote actin-based motility in Rickettsia raoultii. PLoS ONE 3: e2582.
3. Blanc G, Ogata H, Robert C et al 2007) Reductive genome evolution from the mother of Rickettsia. PloS Genet 3: e14.
4. Carlsson F Brown EJ (2006) Actin-based motility of intracellular bacteria, and polarized surface distribution of the bacterial effector molecules. J Cell Physiol 209: 288 296.
5. Gimenez DF (1964) Staining rickettsiae in yolk-sac cultures. Stain Technol 39: 135 140.
6. Gouin E, Gantelet H, Egile C et al 1999) A comparative study of the actin-based motilities of the pathogenic bacteria Listeria monocytogenes, Shigella flexneri and Rickettsia conorii. J Cell Sci 112: 1697 1708.
7. Gouin E, Egile C, Dehoux P et al 2004) The RickA protein of Rickettsia conorii activates the Arp2/3 complex. Nature 427: 457 461.
8. Gouin E, Welch MD Cossart P (2005) Actin-based motility of intracellular pathogens. Curr Opin Microbiol 8: 35 45.
9. Heinzen RA (2003) Rickettsial actin-based motility: behavior and involvement of cytoskeletal regulators. Ann N Y Acad Sci 990: 535 547.
10. Heinzen RA, Hayes SF, Peacock MG Hackstadt T (1993) Directional actin polymerization associated with spotted fever group Rickettsia infection of Vero cells. Infect Immun 61: 1926 1935.
11. Jeng RL, Goley ED, D'Alessio JA et al 2004) A rickettsia WASP-like protein activates the Arp2/3 complex and mediates actin-based motility. Cell Microbiol 6: 761 769.
12. Köhler G Milstein C (1976) Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur J Immunol 6: 511 519.
13. La Scola B Raoult D (1997) Laboratory diagnosis of rickettsioses: current approaches to diagnosis of old and new rickettsial diseases. J Clin Microbiol 35: 2715 2727.
14. McLeod MP, Qin X, Karpathy SE et al 2004) Complete genome sequence of Rickettsia typhi and comparison with sequences of other rickettsiae. J Bacteriol 186: 5842 5855.
15. Ogata H, Audic S, Renesto-Audiffren P et al 2001) Mechanisms of evolution in Rickettsia conorii and R. prowazekii. Science 293: 2093 2098.
16. Ogata H, La Scola B, Audic S et al 2006) Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens. PLoS Genet 2: e76.
17. Parola P, Paddock CD Raoult D (2005) Tick-borne rickettsioses around the world: emerging diseases challenging old concepts. Clin Microbiol Rev 18: 719 756.
18. Raoult D Roux V (1997) Rickettsioses as paradigms of new or emerging infectious diseases. Clin Microbiol Rev 10: 694 719.
19. Renesto P, Azza S, Dolla A et al 2005) Proteome analysis of Rickettsia conorii by two-dimensional gel electrophoresis coupled with mass spectrometry. FEMS Microbiol Lett 245: 231 238.
20. Renesto P, Samson L, Ogata H et al 2006) Identification of two putative rickettsial adhesins by proteomic analysis. Res Microbiol 158: 605 612.
21. Roberts AJ Wiedmann M (2006) Allelic exchange and site-directed mutagenesis probe the contribution of ActA amino-acid variability to phosphorylation and virulence-associated phenotypes among Listeria monocytogenes strains. FEMS Microbiol Lett 254: 300 307.
22. Shen Z, Batzer A, Khoehler JA et al 1999) Evidence for SH3 domain directed binding and phosphorylation of Sam68 by Src. Oncogene 18: 4647 4653.
23. Shevchenko A, Wilm M, Vorm O Mann M (1996) Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem 68: 850 858.
24. Simser JA, Palmer AT, Munderloh UG Kurtti TJ (1999) Isolation and characterization of Rickettsia peacockii maintained in tick cell culture. Am J Trop Med Hyg 61: (suppl.): 359.
25. Stevens JM, Galyov EE Stevens MP (2006) Actin-dependent movement of bacterial pathogens. Nat Rev Microbiol 4: 91 101.
26. Teysseire N, Chiche-Portiche C Raoult D (1992) Intracellular movements of Rickettsia conorii and R. typhi based on actin polymerization. Res Microbiol 143: 821 829.
27. Van Kirk LS, Hayes SF Heinzen RA (2000) Ultrastructure of Rickettsia rickettsii actin tails and localization of cytoskeletal proteins. Infect Immun 68: 4706 4713.