Allelic exchange and site-directed mutagenesis probe the contribution of ActA amino-acid variability to phosphorylation and virulence-associated phenotypes among Listeria monocytogenes strains

FEMS Microbiology Letters

Volumn 254, Issue 2, 2006, Pages 300-307

  Roberts, Angela J ^a   Wiedmann, Martin ^a  

^a Department of Molecular Biology and Genetics   (United States)

Author keywords

ActA; Allelic variation; Listeria monocytogenes; Virulence

Indexed keywords

AMINO ACID; BACTERIAL PROTEIN; PROTEIN ACT A; UNCLASSIFIED DRUG; ACTA PROTEIN, LISTERIA MONOCYTOGENES; MEMBRANE PROTEIN;

ALLELE; ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM ISOLATE; CELL INTERACTION; CONTROLLED STUDY; GENETIC VARIABILITY; HYPOTHESIS; LISTERIA MONOCYTOGENES; MOLECULAR CLONING; MOLECULAR PROBE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; SITE DIRECTED MUTAGENESIS; STRAIN DIFFERENCE; WILD TYPE; ANIMAL; CATTLE; CELL LINE; CHEMISTRY; CLASSIFICATION; GENE DELETION; GENE EXPRESSION REGULATION; GENETICS; HUMAN; METABOLISM; MOUSE; PATHOGENICITY; PHOSPHORYLATION; VIRULENCE;

BACTERIA (MICROORGANISMS); LISTERIA MONOCYTOGENES;

ALLELES; ANIMALS; BACTERIAL PROTEINS; CATTLE; CELL LINE; GENE DELETION; GENE EXPRESSION REGULATION, BACTERIAL; HUMANS; LISTERIA MONOCYTOGENES; MEMBRANE PROTEINS; MICE; MUTAGENESIS, SITE-DIRECTED; PHENOTYPE; PHOSPHORYLATION; VARIATION (GENETICS); VIRULENCE;

EID: 33644870839     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1021/tx0502589     Document Type: Article

References (28)

1. Brundage RA Smith GA Camilli A Theriot JA Portnoy DA 1993 Expression and phosphorylation of the Listeriamonocytogenes ActA protein in mammalian cells Proc Natl Acad Sci USA 90 11890 11894
2. Cai S 2002 DNA sequence-based subtyping and molecular evolution of Listeria and Listeria monocytogenes. Masters thesis. Department of Food Science, Cornell University, Ithaca, NY.
3. Cai S Kabuki DY Kuaye AY Cargioli TG Chung MS Nielsen R Wiedmann M 2002 Rational design of DNA sequence-based strategies for subtyping Listeriamonocytogenes J Clin Microbiol 40 3319 3325
4. Call DR Borucki MK Besser TE 2003 Mixed-genome microarrays reveal multiple serotype and lineage-specific differences among strains of Listeriamonocytogenes J Clin Microbiol 41 632 639
5. Camilli A Portnoy A Youngman P 1990 Insertional mutagenesis of Listeriamonocytogenes with a novel Tn917 derivative that allows direct cloning of DNA flanking transposon insertions J Bacteriol 172 3738 3744
6. Chakraborty T Ebel F Wehland J Dufrenne J Notermans S 1994 Naturally occurring virulence-attenuated isolates of Listeriamonocytogenes capable of inducing long term protection against infection by virulent strains of homologous and heterologous serotypes FEMS Immunol Med Microbiol 10 1 9
7. Doumith M Cazalet C Simoes N Frangeul L Jacquet C Kunst F Martin P Cossart P Glaser P Buchrieser C 2004 New aspects regarding evolution and virulence of Listeriamonocytogenes revealed by comparative genomics and DNA arrays Infect Immun 72 1072 1083
8. Gellin BG Broome CV 1989 Listeriosis JAMA 261 1313 1320
9. Gray MJ Zadoks RN Fortes ED Dogan B Cai S Chen Y Scott VN Gombas DE Boor KJ Wiedmann M 2004 Listeria monocytogenes isolates from foods and humans form distinct but overlapping populations Appl Environ Microbiol 70 5833 5841
10. d'Hauteville H Sansonetti PJ 1992 Phosphorylation of IcsA by cAMP-dependent protein kinase and its effect on intracellular spread of Shigella flexneri Mol Microbiol 6 833 841
11. Horton RM Cai ZL Ho SN Pease LR 1990 Gene splicing by overlap extension tailor-made genes using the polymerase chain reaction Biotechniques 8 528 535
12. Jeffers GT Bruce JL McDonough PL Scarlett J Boor KJ Wiedmann M 2001 Comparative genetic characterization of Listeriamonocytogenes isolates from human and animal listeriosis cases Microbiology 147 1095 1104
13. Kathariou S 2002 Listeria monocytogenes virulence and pathogenicity, a food safety perspective J Food Prot 65 1811 1829
14. Kocks C Gouin E Tabouret M Berche P Ohayon H Cossart P 1992 L. monocytogenes-induced actin assembly requires the actA gene product, a surface protein Cell 68 521 531
15. Leimeister-Wachter M Goebel W Chakraborty T 1989 Mutations affecting hemolysin production in Listeriamonocytogenes located outside the listeriolysin gene FEMS Microbiol Lett 53 23 29
16. Mueller KJ Freitag NE 2005 Pleiotropic enhancement of bacterial pathogenesis resulting from the constitutive activation of the Listeriamonocytogenes regulatory factor PrfA Infect Immun 73 1917 1926
17. Nightingale KK 2005 Evolution and molecular phylogeny of Listeria monocytogenes isolated from human and animal listeriosis cases and foods J Bacteriol 187 5537 5551
18. Norton DM Scarlett JM Horton K Sue D Thimothe J Boor KJ Wiedmann M 2001 Characterization and pathogenic potential of Listeriamonocytogenes isolates from the smoked fish industry Appl Environ Microbiol 67 646 653
19. Olier M Garmyn D Rousseaux S Lemaitre JP Piveteau P Guzzo J 2005 Truncated internalin A and asymptomatic Listeriamonocytogenes carriage in vivo investigation by allelic exchange Infect Immun 73 644 648
20. Roberts A Chan Y Wiedmann M 2005 Definition of genetically distinct attenuation mechanisms in naturally virulence-attenuated Listeria monocytogenes by comparative cell culture and molecular characterization Appl Environ Microbiol 71 3900 3910
21. Skoble J Portnoy DA Welch MD 2000 Three regions within ActA promote Arp2/3 complex-mediated actin nucleation and Listeriamonocytogenes motility J Cell Biol 150 527 538
22. Smith GA Theriot JA Portnoy DA 1996 The tandem repeat domain in the Listeriamonocytogenes ActA protein controls the rate of actin-based motility, the percentage of moving bacteria, and the localization of vasodilator- stimulated phosphoprotein and profilin J Cell Biol 135 647 660
23. Smith K Youngman P 1992 Use of a new integrational vector to investigate compartment-specific expression of the Bacillus subtilis spoIIM gene Biochimie 74 705 711
24. Sun AN Camilli A Portnoy DA 1990 Isolation of Listeriamonocytogenes small-plaque mutants defective for intracellular growth and cell-to-cell spread Infect Immun 58 3770 3778
25. Tilney LG Portnoy DA 1989 Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite, Listeriamonocytogenes J Cell Biol 109 1597 1608
26. Wiedmann M Bruce JL Keating C Johnson AE McDonough PL Batt CA 1997 Ribotypes and virulence gene polymorphisms suggest three distinct Listeriamonocytogenes lineages with differences in pathogenic potential Infect Immun 65 2707 2716
27. Wiedmann M Arvik TJ Hurley RJ Boor KJ 1998 General stress transcription factor sigmaB and its role in acid tolerance and virulence of Listeriamonocytogenes J Bacteriol 180 3650 3656
28. Zhang C Zhang M Ju J et al 2003 Genome diversification in phylogenetic lineages I and II of Listeriamonocytogenes identification of segments unique to lineage II populations J Bacteriol 185 5573 5584