Stratified epithelial sheets engineered from a single adult murine corneal/limbal progenitor cell

Journal of Cellular and Molecular Medicine

Volumn 12, Issue 4, 2008, Pages 1303-1316

  Kawakita, Tetsuya ^a,b,c,d   Shimmura, Shigeto ^b,c   Hornia, Armand ^a   Higa, Kazunari ^b   Tseng, Scheffer C G ^a,d  

^a TISSUETECH INC   (United States)

^b TOKYO DENTAL COLLEGE   (Japan)

^c KEIO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^d OCULAR SURFACE CENTER   (United States)

Author keywords

Cornea; Culture; Epithelium; Regenerative medicine; Senescence and growth; Stem cell

Indexed keywords

CALCIUM; KERATIN;

ANIMAL; ARTICLE; CELL CLONE; CELL COUNT; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SHAPE; CELL STRAIN 3T3; CLONOGENIC ASSAY; CORNEA LIMBUS; CYTOLOGY; DNA FRAGMENTATION; DRUG EFFECT; EPITHELIUM CELL; METABOLISM; MOUSE; PHENOTYPE; SERUM; STEM CELL; TIME; TISSUE ENGINEERING;

3T3 CELLS; ANIMALS; CALCIUM; CELL COUNT; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SHAPE; CLONE CELLS; COLONY-FORMING UNITS ASSAY; DNA FRAGMENTATION; EPITHELIAL CELLS; KERATINS; LIMBUS CORNEAE; MICE; PHENOTYPE; SERUM; STEM CELLS; TIME FACTORS; TISSUE ENGINEERING;

BOVINAE; MURINAE;

EID: 49549112965     PISSN: 15821838     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1582-4934.2008.00297.x     Document Type: Article

References (18)

1. Schermer A, Galvin S, Sun T-T. Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. J Cell Biol. 1986 103 : 49 62.
2. Lavker RM, Sun T-T. Epidermal stem cells: Properties, markers and location. Proc Natl Acad Sci USA. 2000 97 : 13473 5.
3. Romano AC, Espana EM, Yoo SH, Budak MT, Wolosin JM, Tseng SC. Different cell sizes in human limbal and central corneal basal epithelia measured by confocal microscopy and flow cytometry. Invest Ophthalmol Vis Sci. 2003 44 : 5125 9.
4. Cotsarelis G, Cheng SZ, Dong G, Sun T-T, Lavker RM. Existence of slow-cycling lim-bal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells. Cell. 1989 57 : 201 9.
5. Liu C-Y, Zhu G, Converse R, Kao CW, Nakamura H, Tseng SC, Mui MM, Seyer J, Justice MJ, Stech ME. Characterization and chromosomal localization of the cornea-specific murine keratin gene Krt1.12. J Biol Chem. 1994 260 : 24627 36.
6. Matic M, Petrov IN, Chen S, Wang C, Dimitrijevich SD, Wolosin JM. Stem cells of the corneal epithelium lack connexins and metabolite transfer capacity. Differentiation. 1997 61 : 251 60.
7. Pellegrini G, Dellambra E, Golisano O, Martinelli E, Fantozzi I, Bondanza S, Ponzin D, McKeon F, De Luca M. p63 identifies keratinocyte stem cells. Proc Natl Acad Sci USA. 2001 98 : 3156 61.
8. Watanabe K, Nishida K, Yamato M, Umemoto T, Sumide T, Yamamoto K, Maeda N, Watanabe H, Okano T, Tano Y. Human limbal epithelium contains side population cells expressing the ATP-binding cassette transporter ABCG2. FEBS Lett. 2004 565 : 6 10.
9. Hayashi R, Yamato M, Sugiyama H, Sumide T, Yang J, Okano T, Tano Y, Nishida K. N-Cadherin is expressed by putative stem/progenitor cells and melanocytes in the human limbal epithelial stem cell niche. Stem Cells. 2007 25 : 289 96.
10. Lehrer MS, Sun TT, Lavker RM. Strategies of epithelial repair: modulation of stem cell and transit amplifying cell proliferation. J Cell Sci. 1998 10 : 2867 75.
11. Pellegrini G, Golisano O, Paterna P, Lambiase A, Bonini S, Rama P, De Luca M. Location and clonal analysis of stem cells and their differentiated progeny in the human. Ocular Surface. 1999 145 : 769 82.
12. Kawakita T, Espana EM, He H, Yeh LK, Liu CY, Tseng SC. Calcium-induced abnormal epidermal-like differentiation in cultures of mouse corneal-limbal epithelial cells. Invest Ophthalmol Vis Sci. 2004 45 : 3507 12.
13. Yang A, Kaghad M, Wang Y, Gillett E, Fleming MD, Dötsch V, Andrews NC, Caput D, McKeon F. p63, a p53 homolog at 3q27-29, encodes multiple products with transactivating, death-inducing, and dominant-negative activities. Mol Cell. 1998 2 : 305 16.
14. Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell. 1975 6 : 331 43.
15. Kubota T, Koshizuka K, Williamson EA, Asou H, Said JW, Holden S, Miyoshi I, Koeffler HP. Ligand for peroxisome prolif-erator-activated receptor gamma (troglita-zone) has potent antitumor effect against human prostate cancer both in vitro and in vivo. Cancer Res. 1998 58 : 3344 52.
16. Grueterich M, Espana E, Tseng SC. Connexin 43 expression and proliferation of human limbal epithelium on intact and denuded amniotic membrane. Invest Ophthalmol Vis Sci. 2002 43 : 63 71.
17. Barrandon Y, Green H. Three clonal types of keratinocytes with different capacities for multiplication. Proc Natl Acad Sci USA. 1987 84 : 2302 6.
18. Li W, Hayashida Y, Chen YT, Tseng SC. Niche regulation of corneal epithelial stem cells at the limbus. Cell Res. 2007 17 : 26 36.