Injection of poly(β-L-malate) into the plasmodium of Physarum polycephalum shortens the cell cycle and increases the growth rate

European Journal of Biochemistry

Volumn 271, Issue 19, 2004, Pages 3805-3811

  Karl, Michael ^a   Anderson, Roger ^b   Holler, Eggehard ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

Cell cycle; Growth rate; Physarum polycephalum; Plasmodium; Polymalic acid

Indexed keywords

BIOPOLYMER; DEOXYRIBOSE; HISTONE H1; MALIC ACID DERIVATIVE; NUCLEAR PROTEIN; NUCLEIC ACID; PHOSPHODEOXYRIBOSE; POLY(BETA MALATE); RIBOSE PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; CELL CYCLE; CELL NUCLEUS; CONTROLLED STUDY; CYTOPLASM; GROWTH ACCELERATION; GROWTH RATE; MULTINUCLEAR CELL; MYXOMYCETES; NONHUMAN; PHYSARUM POLYCEPHALUM; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN TRANSPORT; STRAIN DIFFERENCE; TRANSCRIPTION REGULATION; TRANSLATION REGULATION;

ANIMALS; CELL CYCLE; CELL DIVISION; CELL NUCLEUS; CYTOPLASM; DNA POLYMERASE I; ENZYME INHIBITORS; HISTONES; INJECTIONS; MALATES; MICROINJECTIONS; PHYSARUM POLYCEPHALUM; PLASMODIUM; POLYMERS; PROTEIN BIOSYNTHESIS; TRANSCRIPTION, GENETIC;

CHONDROMYCES; MYXOGASTRIA; PHYSARUM; PHYSARUM POLYCEPHALUM;

EID: 4944242228     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.2004.04299.x     Document Type: Article

References (27)

1. Burland, T.G., Solnica, K.L., Bailey, J., Cunningham, D.B. & Dove, W.F. (1993) Patterns of inheritance, development and the mitotic cycle in the protist Physarum polycephalum. Adv. Microb. Physiol. 35, 1-69.
2. Fischer, H., Erdmann, E.S. & Holler, E. (1989) An unusual polyanion from Physarum polycephalum that inhibits homologous DNA polymerase α in vitro. Biochemistry 28, 5219-5226.
3. Schmidt, A., Windisch, C. & Holler, E. (1996) Nuclear accumulation and homeostasis of the unusual polymer β-poly(L-malate) in plasmodia of Physarum polycephalum. Eur. J. Cell. Biol. 70, 373-380.
4. Rathberger, K., Reisner, H., Willibald, B.H., Molitoris, H.-P. & Holler, E. (1999) Comparative synthesis and hydrolytic degradation of poly(L-malate) by myxomycetes and fungi. Mycol. Res. 103, 513-520.
5. Holler, E., Achhammer, G., Angerer, B., Gantz, B., Hambach, C., Reisner, H., Seidel, B., Weber, C., Windisch, C., Braud, C. & Vert, M. (1992) Sepcific inhibition of Physarum polycephalum DNA-polymerase-α-primase by poly(β-L-malate) and related polyanions. Eur. J. Biochem. 206, 1-6.
6. Angerer, B. & Holler, E. (1995) Large complexes of beta-poly (L-malate) with DNA polymerase alpha, histones, and other proteins in nuclei of growing plasmodia of Physarum polycephalum. Biochemistry 34, 14741-14751.
7. Achhammer, G., Winkler, A., Angerer, B. & Holler, E. (1995) DNA polymerase δ of Physarum polycephalum. Curr. Genet. 28, 534-545.
8. Doerhoefer, S., Khodyreva, S., Safronov, I.V., Wlassoff, W.A., Anarbaev, R., Lavrik, O.I. & Holler, E. (1998) Molecular constituents of the replication apparatus in the plasmodium of Physarum polycephalum: identification by photoaffinity labelling. Microbiology 144, 3181-3193.
9. Karl, M. & Holler, E. (1998) Multiple polypeptides immunologically related to β-poly(L-malate) hydrolase (polymalatase) in the plasmodium of the slime mold Physarum polycephalum. Eur. J. Biochem. 251, 405-412.
10. Karl, K., Gasselmaier, B., Krieg, R. & Holler, E. (2003) Localization of fluorescence-labeled poly(malic acid) to the nuclei of the plasmodium of Physarum polycephalum. Eur. J. Biochem. 270, 1536-1542.
11. Doerhoefer, S., Windisch, C., Angerer, B., Lavrik, O.I., Lee, B.-S. & Holler, E. (2002) The DNA-polymerase inhibiting activity of poly(β-L-malic acid) in nuclear extract during the cell cycle of Physarum polycephalum. Eur. J. Biochem. 269, 1253-1258.
12. Anderson, R.W. (1977) A plasmodial colour mutation in the myxomycete Physarum polycephalum. Genet. Res. 30, 301-306.
13. Daniel, F.W. & Baldwin, H.H. (1964) Methods of culture for plasmodial myxomycetes. Methods Cell Physiol. 1, 9-14.
14. Nygaard, O.P. & Guttes, S.R.H.P. (1960) Nucleic acid metabolism in a slime mold with synchronous mitosis. Biochim. Biophys. Acta 38, 298-306.
15. Holler, E. (1997) Poly(malic acid) from natural sources. In Handbook of Engineering Polymeric Materials. (Cheremisinof, N.P., ed.), pp. 93-103. Marcel Dekker, New York.
16. Lee, B.-S., Vert, M. & Holler, E. (2002) Water-soluble aliphatic polyesters: poly(malic acid)s. In Biopolymers 3a (Doi, Y. & Steinbüchel, A., eds), pp. 75-103. Wiley-VCH, Weinheim.
17. Albert, P., Lacorre-Arescaldino, I. & Toublan, B. (1993) High resolution polyacrylamide gel electrophoresis of Physarum polycephalum histones: improved resolution of variants and modified forms. Anal. Biochem. 209, 224-227.
18. Mohberg, J. (1982) Recognition of mitosis. In Cell Biology of Physarum and Didymium (Aldrich, H.C. & Daniel, J.W., eds), pp. 273-276. Academic Press, New York.
19. Wright, M., Lacorre-Arescaldino, I., Macquet, J.P. & Daffé, M. (1984) Introduction of polyploid nuclei in the plasmodium of Physarum polycephalum by antitumor compounds. Cancer Res. 44, 777-783.
20. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
21. Loidl, P. & Gröbner, P. (1987) Histone synthesis during the cell cycle of Physarum polycephalum. Synthesis of different histone species is not under a common regulatory control. J. Biol. Chem. 262, 10195-10199.
22. Gold, D.V. & Shochat, D. (1980) A rapid colorimetric assay for the estimation of microgram quantities of DNA. Anal. Biochem. 105, 121-125.
23. Lee, B.-S. & Holler, E. (1999) Effects of culture conditions on β-poly(L-malate) production by Physarum polycephalum. Appl. Microbiol. Biotechnol. 51, 647-652.
24. Sommerville, J. & Ladomery, M. (1996) Transcription and masking of mRNA in germ cells: involvement of Y-box proteins. Chromosoma 104, 469-478.
25. Yu, J., Hecht, N.B. & Schultz, R.M. (2002) Expression of MSY2 in mouse oocytes and preimplantation embryos. Biol. Reprod. 65, 1260-1270.
26. Bleher, R. & Martin, R. (1999) Nucleo-cytoplasmic translocation of histone H1 during HeLa cell cycle. Chromosoma 108, 308-816.
27. Zlatanova, J.S., Srebreva, L.N., Banchev, T.B. & Tsanev, K.G. (1990) Cytoplasmic pool of histone H1 in mammalian cells. J. Cell Sci. 96, 461-465.