Localization of fluorescence-labeled poly(malic acid) to the nuclei of the plasmodium of Physarum polycephalum

European Journal of Biochemistry

Volumn 270, Issue 7, 2003, Pages 1536-1542

  Karl, Miachael ^a   Gasselmaier, Bernd ^a   Krieg, René C ^a   Holler, Eggehard ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

Author keywords

Physarum polycephalum; Plasmodium; Polymalatase; Polymalic acid; Reconstituting nuclei

Indexed keywords

ANTIBODY; FLUORESCEIN ISOTHIOCYANATE; MALATE DEHYDROGENASE; MALIC ACID DERIVATIVE; POLY(MALIC ACID); UNCLASSIFIED DRUG;

ARTICLE; CELL FUNCTION; CELL NUCLEUS; CELLULAR DISTRIBUTION; ENZYME BINDING; FLUORESCENCE; IMMUNOPRECIPITATION; NONHUMAN; PHYSARUM POLYCEPHALUM; PRIORITY JOURNAL; PROTEIN LOCALIZATION; STAINING;

ACTIVE TRANSPORT, CELL NUCLEUS; ANIMALS; CARBOXYLIC ESTER HYDROLASES; CELL NUCLEUS; FLUORESCENT DYES; MACROMOLECULAR SUBSTANCES; MALATES; MICROINJECTIONS; PHYSARUM POLYCEPHALUM; POLYMERS; PRECIPITIN TESTS; PROTOZOAN PROTEINS;

CHONDROMYCES; MYXOGASTRIA; PHYSARUM POLYCEPHALUM; PROTOZOA;

EID: 0037392201     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2003.03521.x     Document Type: Article

References (34)

1. Wick, R.J. & Sauer, H.W. (1982) Developmental biology of slime molds: An overview. In Cell Biology of Physarum and Didymium (Aldrich, H.C. & Daniel, J.W., eds), pp. 3-20. Academic Press, New York, USA.
2. Baldauf, S.L. & Doolittle, W.F. (1997) Origin and evolution of the slime molds (Mycetozoa). Proc. Natl. Acad. Sci. 94, 12007-12012.
3. Burland, T.G., Bailey, J., Adam, L., Mukhopadhyay, M.J., Dove, W.F. & Pallotta, D. (1992) Transient expression in Physarum of a chloramphenicol acetyltransferase gene under the control of actin gene promoters. Curr. Genet. 21, 393-398.
4. Thiriet, C. & Hayes, J.J. (1999) Histone proteins in vivo: Cell-cycle-dependent physiological effects of exogeneous linker histones incorporated into Physarum polycephalum. Methods: a Companion to Methods in Enzymology 17, 140-150.
5. Fischer, H., Erdmann, S. & Holler, E. (1989) An unusual polyanion from Physarum polycephalum that inhibits homologous DNA polymerase α in vitro. Biochemistry 28, 5219-5226.
6. Doehoefer, S., Windisch, C., Angerer, B., Lavrik, O.I., Lee, B.-S. & Holler, E. (2002) The DNA-polymerase inhibiting activity of poly (β-L-malic acid) in nuclear extract during the cell cycle of Physarum polycephalum. Eur. J. Biochem. 269, 1253-1258.
7. Achhammer, G., Angerer, B., Windisch, C., Uhl, A. & Holler, E. (1992) DNA Polymerase α-primase complexes of Physarum polycephalum. Cell. Biol. Int. Reports 16, 1047-1053.
8. Achhammer, G., Winkler, A., Angerer, B. & Holler, E. (1995) DNA polymerase δ of Physarum polycephalum. Curr. Genet. 28, 534-545.
9. Angerer, B. & Holler, E. (1995) Large complexes of β-poly (L-malate) with DNA polymerase α, histones, and other proteins in nuclei of growing plasmodia of Physarum polycephalum. Biochemistry 34, 14741-14751.
10. Doerhoefer, S., Khodyreva, S., Safronov, I.V., Wlassoff, W.A., Anarbaev, R., Lavrik, O.I. & Holler, E. (1998) Molecular consituents of the replication apparatus in the plasmodium of Physarum polycephalum: identification by photoaffinity labelling. Microbiology 144, 3181-3193.
11. Willibald, B., Bildl, W. Lee, B.-S. & Holler, E. (1999) Is β-Poly (L-malate) synthesis catalysed by a combination of β-L-malyl-AMP-ligase and β-poly(L-malate) polymerase? Eur. J. Biochem. 265, 1085-1090.
12. Korherr, C., Roth, M. & Holler, E. (1965) Poly(β-L-malate) hydrolase from plasmodia of Physarum polycephalum. Can. J. Microbiol. 41 (Suppl. 1), 192-199.
13. Schmidt, A., Windisch, C. & Holler, E. (1996) Nuclear accumulation and homeostasis of the unusual polymer β-poly(L-malate) in plasmodia of Physarum polycephalum. Eur. J. Cell. Biol. 70, 373-380.
14. Gasslmaier, B., Krell, C.M., Seebach, D. & Holler, E. (2000) Synthetic substrates and inhibitors of β-poly(L-malate)-hydrolase (polymalatase). Eur. J. Biochem. 267, 5101-5105.
15. Karl, M. & Holler, E. (1998) Multiple polypeptides immunologically related to β-poly(L-malate) hydrolase (polymalatase) in the plasmodium of the slime mold Physarum polycephalum. Eur. J. Biochem. 251, 405-412.
16. Daniel, J.W. & Baldwin, H.H. (1964) Methods of culture for plasmodial myxomycetes. Methods Cell Physiol. 1, 9-14.
17. Nygaard, O.P. & Guttes, S.R.H.P. (1960) Nucleic acid metabolism in a slime mold with synchronous mitosis. Biochim. Biophys. Acta 38, 298-306.
18. Holler, E. (1997) Poly(malic acid) from natural sources. Handbook of Engineering Polymeric Materials (Cheremisinoff, N.P., ed.), pp. 93-103. Marcel Dekker, Inc, New York, USA.
19. Means, G.E. & Feeney, R.E. (1971) Chemical Modification of Proteins. Holden-Day, San Franciso, USA.
20. Geiger, B. & Volberg, T. (1998) Cell Biology. Academic Press, New York, USA.
21. Walter, W. & Bode, K.-D. (1967) Synthesen von Thiourethanen. Angewandt Chemie 79, 285-328.
22. Gasslmaier, B. & Holler, E. (1997) Specificity and direction of depolymerization of β-poly(L-malate) catalysed by polymalatase from Physarum polycephalum. Fluorescence labeling at the carboxy-terminus of β-poly(L-malate). Eur. J. Biochem. 250, 308-314.
23. Laemmli. U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
24. Holler, E., Fischer, H. & Simek, H. (1985) Non-disruptive detection of DNA polymerases in nondenaturing polyacrylamide gels. Eur. J. Biochem. 151, 311-317.
25. Heukeshoven, J.V. & Dernick, R. (1988) Improved silver staining for fast staining in Phast System Development Units. Staining of SDS-Gels. Electrophoresis 2, 28-32.
26. Towbin, H., Staehlin, T. & Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354.
27. Adams, J.C. (1981) Heavy metal intensification of DAB-based HRP reaction product. J. Histochem. Cytochem. 29, 775.
28. Naib-Majani, W., Osborn, M., Weber, K., Wohfarth-Bottermann, K.-E., Hinssen, H. & Stockem, W. (1983) Immunocytochemistry of the acellular slime mold Physarum polycephalum. J. Cell Sci. 60, 13-28.
29. Mohberg, J. (1982) Recognition of mitosis. Cell Biology of Physarum and Didymium (Aldrich, H.C. & Daniel, J.W., eds), pp. 273-276. Academic Press, New York, NY.
30. Prior, C.P., Cantor, C.R., Johnson, E.M. & Allfrey, V.G. (1980) Incorporation of exogenous pyrene-labeled histone into Physarum chromatin: a system for studying changes in nucleosome assembled in vivo. Cell 20, 597-608.
31. Holler, E., Achhammer, G., Angerer, B., Gantz, B., Hambach, C., Reisner, H., Seidel, B., Weber, C., Windisch, C., Braud, C., Guerin, P. & Vert, M. (1992) Specific inhibition of Physarum polycephalum DNA-polymerase-α-primase by poly(L-malate) and related polyanions. Eur. J. Biochem. 206, 1-6.
32. Christensen, M.E. & Fuxa, K.P. (1988) The nucleolar protein B-36 contains a glycine and dimethylarginine-rich sequence conserved in several other nuclear RNA-binding proteins. Biochem. Biophys. Res. Commun. 155, 1278-1283.
33. Trotman, L.C., Mosberger, N., Fornerod, M., Stidwill, R.P. & Greber, U.F. (2001) Import of adenovirus DNA involves the nuclear pore complex receptor CAN/Nup214 and histone H1. Nature Cell Biol. 3, 1092-1100.
34. Lee, B.-S., Vert, M. & Holler, E. (2002) Water-soluble aliphatic polyesters: poly(malic acid)s. In Biopolymers 3a (Doi, Y. & Steinbüchel, A., eds), pp. 75-103. Wiley-VCH, Weinheim (Bergstrasse), Germany.