SPMSP: A solid-phase PCR with colorimetric read-out for quantitative methylation analysis

Anticancer Research

Volumn 28, Issue 4 B, 2008, Pages 2055-2060

  Sievers, Sonja ^a   Fritzsch, Carla ^a   Kuhnen, Cornelius ^b   Müller, Oliver ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

^b RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

PCR ELISA; Promoter hypermethylation; Quantitative DNA methylation analysis; Quantitative PCR; Solid phase PCR

Indexed keywords

DNA;

ADULT; AGED; ARTICLE; CANCER RESEARCH; CLINICAL ARTICLE; COLORECTAL CARCINOMA; COLORIMETRY; CONTROLLED STUDY; EMBRYO; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE AMPLIFICATION; GENE SEQUENCE; HUMAN; HUMAN CELL; HUMAN TISSUE; LABORATORY APPARATUS, EQUIPMENT AND SUPPLIES; METHYLATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TUMOR SUPPRESSOR GENE;

ADULT; AGED; AGED, 80 AND OVER; BASE SEQUENCE; COLORECTAL NEOPLASMS; COLORIMETRY; DNA METHYLATION; DNA, NEOPLASM; HUMANS; MIDDLE AGED; POLYMERASE CHAIN REACTION;

EID: 49049118263     PISSN: 02507005     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Jones PA and Laird PW: Cancer epigenetics comes of age. Nat Genet 21: 163-167, 1999.
2. Jones PA and Baylin SB: The fundamental role of epigenetic events in cancer. Nat Rev Genet 3: 415-428, 2002.
3. Esteller M, Sparks A, Toyota M, Sanchez-Cespedes M, Capella G, Peinado MA, Gonzalez S, Tarafa G, Sidransky D, Meltzer SJ, Baylin SB and Herman JG: Analysis of adenomatous polyposis coli promoter hypermethylation in human cancer. Cancer Res 60: 4366-4371,2000.
4. Esteller M: Relevance of DNA methylation in the management of cancer. Lancet Oncol 4: 351-358, 2003.
5. Frommer M, McDonald LE, Millar DS, Collis CM, Watt F, Grigg GW, Molloy PL and Paul CL: A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands. Proc Natl Acad Sci USA 89: 1827-1831,1992.
6. Herman JG, Graff JR, Myohanen S, Nelkin BD and Baylin SB: Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci USA 93: 9821-9826,1996.
7. Coleman WB and Rivenbark AG: Quantitative DNA methylation analysis: the promise of high-throughput epigenomic diagnostic testing in human neoplastic disease. J Mol Diagn 8: 152-156,2006.
8. Lehmann U, Berg-Ribbe I, Wingen LU, Brakensiek K, Becker T, Klempnauer J, Schlegelberger B, Kreipe H and Flemming P: Distinct methylation patterns of benign and malignant liver tumors revealed by quantitative methylation profiling. Clin Cancer Res 11: 3654-3660, 2005.
9. Martin CS, Butler L and Bronstein I: Quantitation of PCR products with chemiluminescence. Biotechniques 18: 908-913, 1995.
10. Carmon A, Vision TJ, Mitchell SE, Thannhauser TW, Muller U and Kresovich S: Solid-phase PCR in microwells: effects of linker length and composition on tethering, hybridization, and extension. Biotechniques 32: 410-420, 2002.
11. Oroskar AA, Rasmussen SE, Rasmussen HN, Rasmussen SR, Sullivan BM and Johansson A: Detection of immobilized amplicons by ELISA-like techniques. Clin Chem 42: 1547-1555, 1996.
12. Pfaffl MW, Horgan GW and Dempfle L: Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 30: e36, 2002.
13. Grunau C, Clark SJ and Rosenthal A: Bisulfite genomic sequencing: systematic investigation of critical experimental parameters. Nucleic Acids Res 29: E65, 2001.
14. Eads CA, Danenberg KD, Kawakami K, Saltz LB, Blake C, Shibata D, Danenberg PV and Laird PW: MethyLight: A high-throughput assay to measure DNA methylation. Nucleic Acids Res 28: E32, 2000.
15. Thomassin H, Kress C and Grange T: MethylQuant: A sensitive method for quantifying methylation of specific cytosines within the genome. Nucleic Acids Res 32: e168, 2004.
16. Zeschnigk M, Bohringer S, Price EA, Onadim Z, Masshofer L and Lohmann DR: A novel real-time PCR assay for quantitative analysis of methylated alleles (QAMA): analysis of the retinoblastoma locus. Nucleic Acids Res 32: e125, 2004.
17. Cottrell SE, Distler J, Goodman NS, Mooney SH, Kluth A, Olek A, Schwope I, Tetzner R, Ziebarth H and Berlin K: A real-time PCR assay for DNA-methylation using methylation-specific blockers. Nucleic Acids Res 32: e10, 2004.
18. Brena RM, Auer H, Kornacker K, Hackanson B, Raval A, Byrd JC and Plass C: Accurate quantification of DNA methylation using combined bisulfite restriction analysis coupled with the Agilent 2100 Bioanalyzer platform. Nucleic Acids Res 34: e17, 2006.
19. Gonzalgo ML and Jones PA: Quantitative methylation analysis using methylation-sensitive single-nucleotide primer extension (Ms-SNuPE). Methods 27: 128-133, 2002.
20. Karimi M, Johansson S, Stach D, Corcoran M, Grander D, Schalling M, Bakalkin G, Lyko F, Larsson C and Ekstrom TJ: LUMA (LUminometric Methylation Assay)-A high throughput method to the analysis of genomic DNA methylation. Exp Cell Res 312: 1989-1995, 2006.
21. Yan PS, Chen CM, Shi H, Rahmatpanah F, Wei SH, Caldwell CW and Huang TH: Dissecting complex epigenetic alterations in breast cancer using CpG island microarrays. Cancer Res 61: 8375-8380,2001.
22. Safar AM, Spencer H, III, Su X, Coffey M, Cooney CA, Ratnasinghe LD, Hutchins LF and Fan CY: Methylation profiling of archived non-small cell lung cancer: a promising prognostic system. Clin Cancer Res 11: 4400-4405, 2005.
23. Wei SH, Chen CM, Strathdee G, Harnsomburana J, Shyu CR, Rahmatpanah F, Shi H, Ng SW, Yan PS, Nephew KP, Brown R and Huang TH: Methylation microarray analysis of late-stage ovarian carcinomas distinguishes progression-free survival in patients and identifies candidate epigenetic markers. Clin Cancer Res 8: 2246-2252, 2002.