13C MR spectroscopy measurements of glutaminase activity in human hepatocellular carcinoma cells using hyperpolarized 13C-labeled glutamine

Magnetic Resonance in Medicine

Volumn 60, Issue 2, 2008, Pages 253-257

  Gallagher, Ferdia A ^a,b,c   Kettunen, Mikko I ^a,b   Day, Sam E ^a,b   Lerche, Mathilde ^d   Brindle, Kevin M ^a,b,e  

^a CANCER RESEARCH UK CAMBRIDGE RESEARCH INSTITUTE   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^c ADDENBROOKE S HOSPITAL   (United Kingdom)

^d Imagnia AB   (Sweden)

^e Department of Biochemistry ^*  (United Kingdom)

Author keywords

Dynamic nuclear polarization; Glutamine metabolism; Hepatocellular carcinoma; Hyperpolarized 13C

Indexed keywords

CELL PROLIFERATION; CELLS; CYTOLOGY; IMAGING TECHNIQUES; MAGNETIC RESONANCE SPECTROSCOPY; METABOLISM; SPIN POLARIZATION; TUMORS;

DYNAMIC NUCLEAR POLARIZATION; GLUTAMINASE; HEPATOCELLULAR CARCINOMA; HEPATOCELLULAR CARCINOMA CELL; HUMAN HEPATOMA CELLS; HYPERPOLARIZED; IN-VIVO; MR SPECTROSCOPY;

AMINO ACIDS;

CARBON 13; GLUTAMINASE; GLUTAMINE; CARBON; TUMOR MARKER; TUMOR PROTEIN;

AMINO ACID METABOLISM; ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; CELL PROLIFERATION; CELL STRAIN HEPG2; CONTROLLED STUDY; HUMAN; HUMAN CELL; LIVER CELL CARCINOMA; TUMOR GROWTH; CHEMISTRY; COMPUTER ASSISTED DIAGNOSIS; ENZYME ACTIVATION; ENZYMOLOGY; LIVER TUMOR; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; TUMOR CELL LINE;

CARBON RADIOISOTOPES; CARCINOMA, HEPATOCELLULAR; CELL LINE, TUMOR; DIAGNOSIS, COMPUTER-ASSISTED; ENZYME ACTIVATION; GLUTAMINASE; GLUTAMINE; HUMANS; LIVER NEOPLASMS; MAGNETIC RESONANCE SPECTROSCOPY; NEOPLASM PROTEINS; TUMOR MARKERS, BIOLOGICAL;

EID: 49049096675     PISSN: 07403194     EISSN: 15222594     Source Type: Journal    
DOI: 10.1002/mrm.21650     Document Type: Article

References (18)

1. Shulman RG, Brown TR, Ugurbil K, Ogawa S, Cohen SM, den Hollander JA. Cellular applications of 31P and 13C nuclear magnetic resonance. Science 1979;205:160-166.
2. Ross B, Lin A, Harris K, Bhattacharya P, Schweinsburg B. Clinical experience with 13C MRS in vivo. NMR Biomed 2003;16:358-369.
3. Ardenkjaer-Larsen JH, Fridlund B, Gram A, Hansson G, Hansson L, Lerche MH, Servin R, Thaning M, Golman K. Increase in signal-to-noise ratio of > 10,000 times in liquid-state NMR. Proc Natl Acad Sci USA 2003;100:10158-10163.
4. Golman K, Ardenkjaer-Larsen JH, Petersson JS, Mansson S, Leunbach I. Molecular imaging with endogenous substances. Proc Natl Acad Sci U S A 2003;100:10435-10439.
5. Golman K, Zandt RI, Lerche M, Pehrson R, Ardenkjaer-Larsen JH. Metabolic imaging by hyperpolarized 13C magnetic resonance imaging for in vivo tumor diagnosis. Cancer Res 2006;66:10855-10860.
6. Golman K, Petersson JS. Metabolic imaging and other applications of hyperpolarized 13C1. Acad Radiol 2006;13:932-942.
7. Mansson S, Johansson E, Magnusson P, Chai CM, Hansson G, Petersson JS, Stahlberg F, Golman K. 13C imaging - a new diagnostic platform. Eur Radiol 2006;16:57-67.
8. Day SE, Kettunen MI, Gallagher FA, Hu DE, Lerche M, Wolber J, Golman K, Ardenkjaer-Larsen JH, Brindle KM. Detecting tumor response to treatment using hyperpolarized (13)C magnetic resonance imaging and spectroscopy. Nat Med 2007;13:1382-1387.
9. Mann CD, Neal CP, Garcea G, Manson MM, Dennison AR, Berry DP. Prognostic molecular markers in hepatocellular carcinoma: a systematic review. Eur J Cancer 2007;43:979-992.
10. Miller WJ, Baron RL, Dodd GD 3rd, Federle MP. Malignancies in patients with cirrhosis: CT sensitivity and specificity in 200 consecutive transplant patients. Radiology 1994;193:645-650.
11. Bode BP. Recent molecular advances in mammalian glutamine transport. J Nutr 2001;131(9 Suppl):2475S-2485S; discussion 2486S-2477S.
12. Bungard CI, McGivan JD. Glutamine availability up-regulates expression of the amino acid transporter protein ASCT2 in HepG2 cells and stimulates the ASCT2 promoter. Biochem J 2004;382(Pt 1):27-32.
13. Bode BP, Souba WW. Modulation of cellular proliferation alters glutamine transport and metabolism in human hepatoma cells. Ann Surg 1994;220:411-422; discussion 422-424.
14. Bode BP, Fuchs BC, Hurley BP, Conroy JL, Suetterlin JE, Tanabe KK, Rhoads DB, Abcouwer SF, Souba WW. Molecular and functional analysis of glutamine uptake in human hepatoma and liver-derived cells. Am J Physiol Gastrointest Liver Physiol 2002;283:G1062-1073.
15. Collins CL, Wasa M, Souba WW, Abcouwer SF. Determinants of glutamine dependence and utilization by normal and tumor-derived breast cell lines. J Cell Physiol 1998;176:166-178.
16. Medina MA, Sanchez-Jimenez F, Marquez J, Rodriguez Quesada A, Nunez de Castro I. Relevance of glutamine metabolism to tumor cell growth. Mol Cell Biochem 1992;113:1-15.
17. Reitzer LJ, Wice BM, Kennell D. Evidence that glutamine, not sugar, is the major energy source for cultured HeLa cells. J Biol Chem 1979;254:2669-2676.
18. Shields AF, Grierson JR, Dohmen BM, Machulla HJ, Stayanoff JC, Lawhorn-Crews JM, Obradovich JE, Muzik O, Mangner TJ. Imaging proliferation in vivo with [F-18]FLT and positron emission tomography. Nat Med 1998;4:1334-1336.