In vitro electron paramagnetic resonance characterization of free radicals: Relevance to exercise-induced lipid peroxidation and implications of ascorbate prophylaxis

Free Radical Research

Volumn 42, Issue 4, 2008, Pages 379-386

  Davison, Gareth W ^a   Ashton, Tony ^b   Davies, Bruce ^c   Bailey, Damian M ^c  

^a UNIVERSITY OF ULSTER   (United Kingdom)

^b UNIVERSITY OF LIVERPOOL   (United Kingdom)

^c UNIVERSITY OF GLAMORGAN   (United Kingdom)

Author keywords

Ascorbic acid; Electron paramagnetic resonance spectroscopy; Free radicals; Lipid peroxidation; Spin trapping

Indexed keywords

ARACHIDONIC ACID; ASCORBIC ACID; FREE RADICAL; LINOLEIC ACID; LINOLENIC ACID; N TERT BUTYL ALPHA PHENYLNITRONE; OLEIC ACID; PHOSPHATIDYLCHOLINE; PLACEBO; POLYUNSATURATED FATTY ACID; AMINE OXIDE; MALONALDEHYDE; PHENYL-N-TERT-BUTYLNITRONE;

ANTIOXIDANT ACTIVITY; ARTICLE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DOUBLE BLIND PROCEDURE; DRUG MECHANISM; ELECTRON SPIN RESONANCE; EXERCISE; HUMAN; HUMAN EXPERIMENT; IN VITRO STUDY; INCUBATION TEMPERATURE; INCUBATION TIME; LIPID PEROXIDATION; MALE; NORMAL HUMAN; OXIDATIVE STRESS; RANDOMIZED CONTROLLED TRIAL; REST; ROOM TEMPERATURE; VENOUS BLOOD; CHEMISTRY; KINETICS; METHODOLOGY;

ALPHA-LINOLENIC ACID; ARACHIDONIC ACID; ASCORBIC ACID; CYCLIC N-OXIDES; ELECTRON SPIN RESONANCE SPECTROSCOPY; EXERCISE; FREE RADICALS; HUMANS; KINETICS; LINOLEIC ACID; LIPID PEROXIDATION; MALE; MALONDIALDEHYDE; OXIDATIVE STRESS; PHOSPHATIDYLCHOLINES;

EID: 48849104821     PISSN: 10715762     EISSN: 10292470     Source Type: Journal    
DOI: 10.1080/10715760801976618     Document Type: Article

References (26)

1. Pryor, WA, Prier, DG and Church, DF. (1981) Radical production from the interaction of ozone and PUFA as demonstrated by electron spin resonance spin-trapping techniques. Environ Res, 24, pp. 42-52.
2. Stolze, K., Udilova, N. and Nohl, H. (2000) Spin trapping of lipid radicals with DEPMPO-derived spin traps: Detection of superoxide, alkyl and alkoxyl radicals in aqueous and lipid phase. Free Radic Biol Med, 29, pp. 1005-1014.
3. Duthie, GG. (1993) Lipid peroxidation. Eur J Clin Nut, 47, pp. 759-764.
4. Tappel, AL. (1973) Lipid peroxidation damage to cell components. Fed Proc, 32, pp. 1870-1874.
5. Frei, B., England, E. and Ames, BN. (1989) Ascorbate is an outstanding antioxidant in human plasma. Proc Natl Acad Sci, 86, pp. 6377-6381.
6. Bendich, A., Machlin, LJ and Scandurra, O. (1986) The antioxidant role of vitamin C. Free Radic Biol Med, 2, pp. 419-444.
7. Halliwell, B. and Gutteridge, JMC. (1999) Free radicals in biology and medicine, Oxford University Press, New York, USA
8. Ashton, T., Rowlands, CC, Jones, E., Young, IS, Jackson, SK, Davies, B. and Peters, JR. (1998) Electron spin resonance spectroscopic detection of oxygen-centred radicals in human serum following exhaustive exercise. Eur J Appl Physiol, 77, pp. 498-502.
9. Davison, GW, George, L., Jackson, SK, Davies, B., Young, IS, Peters, JR, Bailey, DM and Ashton, T. (2002) Exercise, free radicals, and lipid peroxidation in type 1 diabetes mellitus. Free Radic Biol Med, 33, pp. 1543-1551.
10. Davison, GW, Morgan, RM, Hiscock, N., Garcia, JM, Grace, F., Boisseau, N., Davies, B., Castell, L., McEneny, J., Young, IS, Hullin, D., Ashton, T. and Bailey, DM. (2006) Manipulation of systemic oxygen flux by acute exercise and normobaric hypoxia: Implications for reactive oxygen species generation. Clin Sci, 110, pp. 133-141.
11. Bailey, DM, Lawrenson, L., McEneny, J., Young, IS, James, PE, Jackson, SK, Henry, RR, Mathieu-Costello, O., McCord, JM and Richardson, RS. (2007) Electron paramagnetic spectroscopic evidence of exercise-induced free radical accumulation in human skeletal muscle. Free Radic Res, 41, pp. 182-190.
12. 2 catalysts subject to redox treatment. Res Chem Intermed, 29, pp. 793-804.
13. Young, IS and Trimble, ER. (1991) Measurement of malondialdehyde in plasma by high performance liquid chromatography with fluorimetric detection. Ann Clin Biochem, 28, pp. 504-508.
14. Jackson, MJ. (1999) An overview of the methods for assessment of free radical activity in biology. Proc Nutr Soc, 58, pp. 1001-1006.
15. Suzuki, K., Sato, H., Kikuchi, T., Abe, T., Nakaji, S., Sugawara, K., Totsuka, M., Sato, K. and Yamaya, K. (1996) Capacity of circulating neutrophils to produce reactive oxygen species after exhaustive exercise. J Appl Physiol, 8, pp. 1213-1222.
16. Ashton, T., Young, IS, Peters, JR, Jones, E., Jackson, SK, Davies, B. and Rowlands, CC. (1999) Electron spin resonance spectroscopy, exercise, and oxidative stress: An ascorbic acid intervention study. J Appl Physiol, 87, pp. 2032-2036.
17. Bailey, DM, Raman, S., McEneny, J., Young, IS, Parham, KL, Hullin, DA, Davies, B., McKeeman, G., McCord, JM and Lewis, MH. (2006) Vitamin C prophylaxis promotes oxidative lipid damage during surgical ischemia-reperfusion. Free Radic Biol Med, 40, pp. 591-600.
18. Dikalov, S. and Mason, RP. (2001) Spin trapping of polyunsaturated fatty acid-derived peroxyl radical: Reassignment to alkoxyl radical adducts. Free Radic Biol Med, 30, pp. 187-197.
19. Iwahashi, H., Parker, CE, Mason, RP and Tomer, KB. (1991) Radical adducts of nitrosobenzene and 2-methyl-2-nitrosopropane with 12,13-epoxylinoleic acid radical, 12,13-epoxylinolenic acid radical and 14,15-epoxyarachidonic acid radical. Biochem J, 276, pp. 447-453.
20. 2: Involvement of α-tocopherol and phosphatidylcholine alkoxyl radicals. Chem Res Toxicol, 12, pp. 1173-1181.
21. Buettner, GR. (1987) Spin trapping: ESR parameters of spin adducts. Free Radic Biol Med, 3, pp. 259-303.
22. Mason, RP, Kalyanaraman, B., Tainer, BE and Eling, TE. (1980) A carbon-centred free radical intermediate in the prostaglandin synthetase oxidation of arachidonic acid. J Biol Chem, 255, pp. 5019-5022.
23. Niki, E., Saito, T., Kawakami, A. and Kamiya, Y. (1984) Inhibition of oxidation of methyl linoleate in solution by vitamin E and vitamin C. J Biol Chem, 259, pp. 4177-4182.
24. Frei, B., England, E. and Ames, BN. (1989) Ascorbate is an outstanding antioxidant in human plasma. Proc Natl Acad Sci, 86, pp. 6377-6381.
25. Scarpa, M., Rigo, A., Maiorino, M., Ursini, F. and Gregolin, C. (1984) Formation of α-tocopherol radical and recycling of α-tocopherol by ascorbate during peroxidation of phosphatidylcholine liposomes. Biochim Biophys Acta, 801, pp. 215-219.
26. Leung, HW, Vang, NJ and Mavis, RD. (1981) The co-operative interaction between vitamin E and vitamin C in suppression of peroxidation of membrane phospholipids. Biochim Biophys Acta, 664, pp. 266-272.