Crystal structure of the cell wall anchor domain of MotB, a stator component of the bacterial flagellar motor: Implications for peptidoglycan recognition

Proceedings of the National Academy of Sciences of the United States of America

Volumn 105, Issue 30, 2008, Pages 10348-10353

  Roujeinikova, Anna ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Bacterial motility; Molecular mechanism; X ray crystallography

Indexed keywords

BACTERIAL PROTEIN; M PROTEIN; MURAMIC ACID; N ACETYLMURAMIC ACID; OUTER MEMBRANE PROTEIN A; PEPTIDOGLYCAN; PROTEIN MOTA; PROTEIN MOTB; PROTEIN MOTC;

ARTICLE; BACTERIAL CELL WALL; BACTERIAL FLAGELLUM; BETA SHEET; BINDING SITE; COMPLEX FORMATION; CONTROLLED STUDY; CROSS LINKING; CRYSTAL STRUCTURE; DIMERIZATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN STRUCTURE; TORQUE;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BINDING SITES; CELL WALL; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; DISACCHARIDES; FLAGELLA; HELICOBACTER PYLORI; MOLECULAR SEQUENCE DATA; MURAMIC ACIDS; PEPTIDOGLYCAN; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; SEQUENCE HOMOLOGY, AMINO ACID;

BACTERIA (MICROORGANISMS);

EID: 48749111115     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0803039105     Document Type: Article

References (40)

1. DeRosier DJ (1998) The turn of the screw: The bacterial flagellar motor. Cell 93:17-20.
2. Kojima S, Blair DF (2004) Solubilization and purification of the MotA/MotB complex of Escherichia coli. Biochemistry 42:26-34.
3. Khan S, Dapice M, Reese TS (1988) Effects of mot gene expression on the structure of the flagellar motor. J Mol Biol 202:575-584.
4. Manson MD, Tedesco P, Berg HC, Harold FM, Van Der Drift C (1977) A protonmotive force drives bacterial flagella. Proc Natl Acad Sci USA 74:3060-3064.
5. Blair DF, Berg HC (1988) Restoration of torque in defective flagellar motors. Science 242:1678-1681.
6. Kojima S, Blair DF (2001) Conformational change in the stator of the bacterial flagellar motor. Biochemistry 40:13041-13050.
7. Zhou J, et al. (1998) Function of protonatable residues in the flagellar motor of the Eschericia coli: A critical role for Asp-32 of MotB. J Bacteriol 180:2729-2735.
8. Chun SY, Parkinson JS (1988) Bacterial motility: Membrane topology of the Escherichia coli MotB protein. Science 239:276-278.
9. Sharp LL, Zhou J, Blair DF (1995) Tryptophan-scanning mutagenesis of the MotB protein of Escherichia coli. Biochemistry 34:9166-9171.
10. DeMot R, Vanderleydon J (1994) The C-terminal sequence conservation between OmpA-related outer membrane proteins and MotB suggests a common function in both gram-positive and gram-negative bacteria, possibly in the interaction of these domains with peptidoglycan. Mol Microbiol 12:333-334.
11. Blair DF, Kim DY, Berg HC (1991) Mutant MotB proteins in Escherichia coli. J Bacteriol 173:4049-4055.
12. Marshall, BJ, Warren, R (1984) Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet i:1311-1315.
13. Uemura N, et al. (2001) Helicobacter pylori infection and the development of gastric cancer. N Engl J Med 345:784-789.
14. Tomb JF, et al. (1997) The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature 338:539-547.
15. Eaton KA, Morgan DR, Krakowka S (1989) Campylobacter pylori virulence factors in gnotobiotic piglets. Infect Immun 57:1119-1125.
16. Eaton KA, Morgan DR, Krakowka S (1992) Motility as a factor in the colonization of gnotobiotic piglets by Helicobacter pylori. J Med Microbiol 37:123-127.
17. Ottemann KM, Lowenthal AC (2002) Helicobacter pylori uses motility for initial colonization and to attain robust infection. Infect Immun 70:1984-1990.
18. Costa K, et al. (1999) The morphological transition of Helicobacter pylori cells from spiral to coccoid is preceded by a substantial modification of the cell wall. J Bacteriol 181:3710-3715.
19. Roujeinikova A (2008) Cloning, purification, and preliminary x-ray analysis of the C-terminal domain of Helicobacter pylori MotB. Acta Crystallogr F 64:277-280.
20. DeLano, WL (2003) The PyMOL Molecular Graphics System: Version 0.90 (DeLano Scientific, Palo Alto, CA).
21. Berman HM, et al. (2000) The Protein Data Bank. Nucleic Acids Res 28:235-242.
22. Holm L, Sander C (1996) Mapping the protein universe. Science 273:595-603.
23. Parsons LM, Lin F, Orban, J. (2006) Peptidoglycan recognition by Pal, an outer membrane lipoprotein. Biochemistry 45:2122-2128.
24. Grizot S, Buchanan SK (2004) Structure of the OmpA-like domain of RmpM from Neisseria meningitidis. Mol Microbiol 51:1027-1037.
25. Thompson JD, Higgins DG, Gibson TJ (1997) CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties, and weight matrix choice. Nucleic Acids Res 22:4673-4680.
26. Gouet P, Courcelle E, Stuart DI, Metoz F (1999) ESPript: Analysis of multiple sequence alignments in PostScript. Bioinformatics 15:305-308.
27. Collaborative Computational Project No. 4 (1994) The CCP4 suite: Programs for protein crystallography. Acta Crystallogr D 50:760-763.
28. Jones S, Thornton JM (1995) Protein-protein interfactions: A review of protein dimer structures. Prog Biophys Mol Biol 63:31-65.
29. Kojima S, Furukawa Y, Matsunami H, Minamino T, Namba K (2008) Characterization of the periplasmic domain of MotB and implications for its role in the stator assembly of the bacterial flagellar motor. J Bacteriol 190:3314-3322.
30. Cascales E, Bernadac A, Gavioli M, Lazzaroni J-C, Lloubes R (2002) Pal lipoprotein of Escherichia coli plays a major role in outer membrane integrity. J Bacteriol 184:754-759.
31. Meroueh SO, et al. (2006) Three-dimensional structure of the bacterial cell wall peptidoglycan. Proc Natl Acad Sci USA 103:4404-4409.
32. Koch AL (2000) Simulation of the conformation of the murein fabric: The oligoglycan, penta-muropeptide, and cross-linked nona-peptide. Arch Microbiol 174:429-439.
33. Quintela JC, Caparrós M, de Pedro MA (1995) Variability of peptidoglycan structural parameters in gram-negative bacteria. FEMS Microbiol Lett 125:95-100.
34. Leslie AGW (1992) Recent changes to the MOSFLM package for processing film and image plate data. Jnt CCP4/ESF-EACBM Newsl Protein Crystallogr No. 26.
35. Schneider TR, Sheldrick GM (2002) Substructure solution with SHELXD. Acta Crystallogr D 58:1772-1779.
36. Cowtan K (1994) DM: An automated procedure for phase improvement by density modification. Jnt CCP4 ESF-EACBM Newsl Protein Crystallogr 31:34-38.
37. Emsley P, Cowtan K (2004) Coot: Model-building tools for molecular graphics. Acta Crystallogr 60:2126-2132.
38. Read RJ (1986) Improved Fourier coefficients for maps using phases from partial structures with errors. Acta Crystallogr A 42:140-149.
39. Perrakis A, Morris R, Lamzin VS (1999) Automated protein model building combined with iterative structure refinement. Nat Struct Biol 6:458-463.
40. Garcia de la Torre J, Huertas ML, Carrasco B (2000) Calculation of hydrodynamic properties of globular proteins from their atomic-level structure. Biophys J 78:719-730.