Site-directed mutagenesis of the Pseudomonas aeruginosa type III secretion system protein PscJ reveals an essential role for surface-localized residues in needle complex function

Microbial Pathogenesis

Volumn 45, Issue 3, 2008, Pages 225-230

  Burns, Robyn E ^a   McDaniel Craig, Ashley ^a   Sukhan, Anand ^a  

^a OKLAHOMA STATE UNIVERSITY   (United States)

Author keywords

Needle complex; PscJ; Pseudomonas aeruginosa; Type III secretion

Indexed keywords

BACTERIAL PROTEIN; ESSENTIAL AMINO ACID; MUTANT PROTEIN; NONESSENTIAL AMINO ACID; PROTEIN PRGK; PROTEIN PSCJ; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; ESCHERICHIA COLI; GENE TARGETING; GENETIC COMPLEMENTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PSEUDOMONAS AERUGINOSA; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; TYPE III SECRETION SYSTEM;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; MEMBRANE PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN TRANSPORT; PSEUDOMONAS AERUGINOSA;

PSEUDOMONAS AERUGINOSA;

EID: 48749087882     PISSN: 08824010     EISSN: 10961208     Source Type: Journal    
DOI: 10.1016/j.micpath.2008.05.002     Document Type: Article

References (20)

1. Cornelis G.R. The type III secretion injectisome. Nat Rev Microbiol 4 (2006) 811-825
2. Galán J.E., and Wolf-Watz H. Protein delivery into eukaryotic cells by type III secretion machines. Nature 444 (2006) 567-573
3. Kubori T., Matsushima Y., Nakamura D., Uralil J., Lara-Tejero M., Sukhan A., et al. Supramolecular structure of the Salmonella typhimurium type III protein secretion system. Science 280 (1998) 602-605
4. Kubori T., Sukhan A., Aizawa S.I., and Galán J.E. Molecular characterization and assembly of the needle complex of the Salmonella typhimurium type III protein secretion system. Proc Natl Acad Sci U S A 97 (2000) 10225-10230
5. Kimbrough T.G., and Miller S.I. Contribution of Salmonella typhimurium type III secretion components to needle complex formation. Proc Natl Acad Sci U S A 97 (2000) 11008-11013
6. Aizawa S.I. Bacterial flagella and type III secretion systems. FEMS Microbiol Lett 21 (2001) 157-164
7. Crepin V.F., Prasannan S., Shaw R.K., Wilson R.K., Creasey E., Abe C.M., et al. Structural and functional studies of the enteropathogenic Escherichia coli type III needle complex protein EscJ. Mol Microbiol 55 (2005) 1658-1670
8. Yip C.K., Kimbrough T.G., Felise H.B., Vuckovic M., Thomas N.A., Pfuetzner R.A., et al. Structural characterization of the molecular platform for type III secretion system assembly. Nature 435 (2005) 702-707
9. Hauser A.R., and Engel J.N. Pseudomonas aeruginosa induces type-III-secretion-mediated apoptosis of macrophages and epithelial cells. Infect Immun 67 (1999) 5530-5537
10. Kang P.J., Hauser A.R., Apodaca G., Fleiszig S.M., Wiener-Kronish J., Mostov K., et al. Identification of Pseudomonas aeruginosa genes required for epithelial cell injury. Mol Microbiol 24 (1997) 1249-1262
11. Miyata S., Casey M., Frank D.W., Ausubel F.M., and Drenkard E. Use of the Galleria mellonella caterpillar as a model host to study the role of the type III secretion system in Pseudomonas aeruginosa pathogenesis. Infect Immun 71 (2003) 2404-2413
12. Pukatzki S., Kessin R.H., and Mekalanos J.J. The human pathogen Pseudomonas aeruginosa utilizes conserved virulence pathways to infect the social amoeba Dictyostelium discoideum. Proc Natl Acad Sci U S A 99 (2002) 3159-3164
13. Yahr T.L., Goranson J., and Frank D.W. Exoenzyme S of Pseudomonas aeruginosa is secreted by a type III pathway. Mol Microbiol 22 (1996) 991-1003
14. Schweizer H.P. A method for construction of bacterial hosts for lac-based cloning and expression vectors: alpha-complementation and regulated expression. Biotechniques 17 (1994) 452-456
15. Arora S.K., Ritchings B.W., Almira E.C., Lory S., and Ramphal R. A transcriptional activator, FleQ, regulates mucin adhesion and flagellar gene expression in Pseudomonas aeruginosa in a cascade manner. J Bacteriol 179 (1997) 5574-5581
16. Hoang T.T., Karkhoff-Schweizer R.R., Kutchma A.J., and Schweizer H.P. A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 212 (1998) 77-86
17. Sukhan A., Kubori T., and Galán J.E. Synthesis and localization of the Salmonella SPI-1 type III secretion needle complex proteins PrgI and PrgJ. J Bacteriol 185 (2003) 3480-3483
18. Chuanchuen R., Narasaki C.T., and Schweizer H.P. Benchtop and microcentrifuge preparation of Pseudomonas aeruginosa competent cells. Biotechniques 33 (2002) 762-763
19. Sambrook J., and Russell D.W. Molecular cloning: a laboratory manual. 3rd ed. (2001), Cold Spring Harbor Press, Cold Spring Harbor, NY
20. Schweizer H.P., Klassen T.R., and Hoang T. Improved methods for gene analysis and expression in Pseudomonas. In: Nakazawa T., Furukawa K., Haas D., and Silver S. (Eds). Molecular Biology of Pseudomonads (1996), American Society for Microbiology, Washington, DC 229-237