Structure-function analysis of hepatitis C virus envelope-CD81 binding

Journal of Virology

Volumn 74, Issue 10, 2000, Pages 4824-4830

  Petracca, Roberto ^a   Falugi, Fabiana ^a   Galli, Giuliano ^a   Norais, Nathalie ^a   Rosa, Domenico ^a   Campagnoli, Susanna ^a   Burgio, Vito ^b   Di Stasio, Enrico ^c   Giardina, Bruno ^c   Houghton, Michael ^d   Abrignani, Sergio ^a   Grandi, Guido ^a  

^a CHIRON VACCINES   (Italy)

^b POLICLINICO UMBERTO I   (Italy)

^c UNIVERSITY OF CHIETI   (Italy)

^d NOVARTIS PHARMA AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CD81 ANTIGEN;

ANTIBODY AFFINITY; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; HEPATITIS C VIRUS; INTERNALIZATION; NONHUMAN; PRIORITY JOURNAL; VIRUS ENVELOPE;

AMINO ACID SEQUENCE; ANTIBODY AFFINITY; ANTIGENS, CD; CYSTEINE; DISULFIDES; HEPACIVIRUS; HUMANS; LIVER; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP; TUMOR CELLS, CULTURED; VIRAL ENVELOPE PROTEINS;

EID: 17944385968     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.74.10.4824-4830.2000     Document Type: Article

References (18)

1. Altekar, W. 1997. Fluorescence of proteins in aqueous neutral salt solutions. II. Influence of monovalent cation chlorides, particularly cesium chloride. Biopolymers 16:369-386.
2. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
3. Casasnovas, J. M., and T. A. Springer. 1995. Kinetics and thermodynamics of virus binding to receptor. Studies with rhinovirus, intercellular adhesion molecule-1 (ICAM-1), and surface plasmon resonance. J. Biol. Chem. 270: 13216-13224.
4. Chapman, B. S., R. M. Thayer, K. A. Vincent, and N. L. Haigwood. 1991. Effect of intron A from human cytomegalovirus (Towne) immediate-early gene on heterologous expression in mammalian cells. Nucleic Acids Res. 19:3979-3986.
5. Choo, Q.-L., G. Kuo, A. J. Weiner, L. R. Overby, D. W. Bradley, and M. Houghton. 1989. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 244:359-362.
6. Eftink, M. R. 1997. Fluorescence methods for studying equilibrium macromolecule-ligand interactions. Methods Enzymol. 278:221-257.
7. Houghton, M. 1996. Hepatitis C viruses, p. 1035-1058. In B. N. Fields, D. M. Knipe, and P. M. Howley (ed.), Virology. Lippincott-Raven, Philadelphia, Pa.
8. Ishii, K., D. Rosa, Y. Watanabe, T. Katayama, H. Harada, C. Wyatt, K. Kiyosawa, H. Aizaki, Y. Matsuura, M. Houghton, S. Abrignani, and T. Miyamura. 1998. High titers of antibodies inhibiting the binding of envelope to human cells correlate with natural resolution of chronic hepatitis C. Hepatology 28:1117-1120.
9. Lanzavecchia, A., E. Roosneck, T. Gregory, P. Berman, and S. Abrignani. 1988. T cells can present antigens such as HIV gp120 targeted to their own surface molecules. Nature 334:530-532.
10. Levy, S., S. C. Todd, and H. T. Maecker. 1998. CD81 (TAPA-I): a molecule involved in signal transduction and cell adhesion in the immune system. Annu. Rev. Immunol. 16:89-109.
11. Lohman, T. M., and W. Bujalowski. 1991 Thermodynamic methods for model-independent determination of equilibrium binding isotherms for protein-DNA interactions: spectroscopic approaches to monitor binding. Methods Enzymol. 208:258-290.
12. Moore, J. P. 1990. Simple methods for monitoring HIV-1 and HIV-2 gp120 binding to soluble CD4 by enzyme-linked immunosorbent assay: HIV-2 has a 25-fold lower affinity than HIV-1 for soluble CD4. AIDS 4:297-305.
13. Moore, J. P., J. A. McKeating, W. A. Norton, and Q. J. Sattentau. 1991. Direct measurement of soluble CD4 binding to human immunodeficiency virus type 1 virions: gp120 dissociation and its implications for virus-cell binding and fusion reactions and their neutralization by soluble CD4. J. Virol. 65:1133-1140.
14. Pileri, P., Y. Uematsu, S. Campagnoli, G. Galli, F. Falugi, R. Petracca, A. J. Weiner, M. Houghton, D. Rosa, G. Grandi, and S. Abrignani. 1998. Binding of hepatitis C virus to CD81. Science 282:938-941.
15. Riddles, P. W., R. L. Blakeley, and B. Zerner. 1983. Reassessment of Ellman's reagent. Methods Enzymol. 91:49-60.
16. Rosa, D., S. Campagnoli, C. Moretto, E. Guenzi, L. Cousens, M. Chin, C. Dong, A. J. Weiner, J. Y. N. Lau, Q.-L. Choo, D. Chien, P. Pileri, M. Houghton, and S. Abrignani. 1996. A quantitative test to estimate neutralizing antibodies to the hepatitis C virus: cytofluorimetric assessment of envelope glycoprotein 2 binding to target cells. Proc. Natl. Acad. Sci. USA 93:1759-1763.
17. Sambrook, J., E. F. Fritsch, and T. Manialis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
18. World Health Organization. 1997. Hepatitis C. Weekly Epidemiol. Rec. 72:65-69.